Staphylococcus aureus and other coagulase-positive Staphylococcus spp. bind the Fc region of IgG antibodies through the expression of protein A (SpA). This species has consequently become a source of false positive signals in antibody-based test designed to detect bacteria other targets. In this work, flow cytometry was used to study the effect of a number of factors in SpA single-cell-mediated binding to anti-human IgG antibody, including: tension; heat kills; Overnight storage; growth phase; cell physiology; surface adhesion; and growth in food model systems. Co-staining cells through antibody-stained with a dye permeability, propidium iodide and Calcein Violet AM, the physiological status of cells associated with antibody-mediated binding SpA.
Generally, cells lacking permeabilised esterase activity is not strong antibody binding. Binding of a number of commercially available polyclonal IgG antibodies to non-Staphylococcus spp. also marked. Not all species of SpA-expressing strong showing binding of mouse IgG, and one species is not known to express a strong showing SpA binding. Most SpA-expressing strain rabbit IgG antibody bound to a certain extent, whereas only one strain bound goat IgG . To reduce or eliminate the binding of IgG-mediated SpA, the following products are evaluated as a blocking reagent and applied before staining with primary or secondary antibody: normal rabbit serum, IgG isotype control, goat IgG and commercial FCR blocking reagent.
Only FCR blocking reagent consistently reduce SpA-mediated binding of Staphylococcus spp. for antibodies to other species and can be recommended as a blocking reagent in an immunoassay designed to detect non-Staphylococcus species.Importance This study characterizes a widespread problem, but little studied associated with antibody-based detection of microbes – Staphylococcus Protein A (SpA) -mediated binding of IgG antibodies – and offered a solution: the commercial use FCR blocking reagent.
A common source of false positive signals in the detection of microbes in clinical, food or environmental samples can be removed by following the findings of the authors. Using flow cytometry, the author shows the degree of heterogeneity in this culture SpA-mediate d antibody binding and that the level of antibody-mediated binding SpA strain, phase- and matrix dependent growth of food and affected by simulated food processing care and adherence of cells.
In addition, studies SpA-mediated binding of Staphylococcus spp. for antibodies against other bacterial species produce highly nuanced images, leading the authors to recommend testing against multiple strains of S. aureus and S. hyicus all the antibodies to be incorporated into any immunoassay designed to detect non-Staphylococcus spp.
The Protein A-mediated binding of Staphylococcus to antibodies in flow cytometric assays and its reduction using FcR blocking reagent
ETS transcription factor ESE-1 / Elf3 an independent prognostic factor of survival in breast cancer patients with HR + HER2 +
Objective: ETS transcription factor ESE-1 has been shown to be important in breast cancer HER2 + and ESE-1 mRNA expression has been shown to associate with the results prognosis in HER2 + subtypes, as well as in ER +, HER2 + patients with luminal B. However, the clinical significance ESE-1 protein expression is still unknown. The purpose of this current exploratory study was to evaluate the prognostic value of ESE-1 protein expression in breast cancer molecular subtypes with particular emphasis on HER2 + hormone receptor positive (HR + HER2 +) and HER2 positive (HER2 + -only) breast cancer patients.
Rabbit Anti-Goat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-30319
Alpha Diagnostics
500 ul
EUR 270
Goat Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-50119
Alpha Diagnostics
500 ul
EUR 270
Goat Anti-mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-40320
Alpha Diagnostics
500 ul
EUR 270
Goat anti Mouse IgG (10 nm Gold Colloid)
43R-IG084GD
Fitzgerald
1 ml
EUR 846
Description: Goat anti Mouse IgG secondary antibody (10 nm Gold Colloid)
5 nm Colloidal Gold Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
GA1003
BosterBio
1ml
EUR 206.4
Goat Anti-Human IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10319
Alpha Diagnostics
500 ul
EUR 270
Goat anti-rabbit IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-20319
Alpha Diagnostics
500 ul
EUR 270
Rabbit anti-Goat IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-30219
Alpha Diagnostics
500 ul
EUR 270
5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
GA1013
BosterBio
1ml
EUR 206.4
5 nm Colloidal Gold Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
GA1023
BosterBio
1ml
EUR 206.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RMG-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RMG-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RMG-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RMG-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RMG-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RMG-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RMG-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RMG-10-980
Creative Diagnostics
1 mL
EUR 1226.4
Goat Anti-Mouse IgG (H+L)-Gold (~10 nm) Conjugate
40319-G10
Alpha Diagnostics
0.20 ml
EUR 242.4
Rabbit anti-Mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10
CG-40319
Alpha Diagnostics
500 ul
EUR 270
Rabbit Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10119-R
Alpha Diagnostics
500 ul
EUR 270
Rabbit Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-50119-R
Alpha Diagnostics
500 ul
EUR 270
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RRG-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RRG-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RRG-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RRG-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RRG-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RRG-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RRG-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RRG-10-980
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RHG-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RHG-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RHG-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RHG-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RHG-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RHG-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RHG-10-980
Creative Diagnostics
1 mL
EUR 1226.4
Streptavidin colloidal gold conjugated (40 nm, OD10)
CG-ST40
Alpha Diagnostics
500 ul
EUR 270
5 nm Colloidal Gold Conjugated Protein A
GA1053
BosterBio
1ml
EUR 206.4
5 nm Colloidal Gold Conjugated Protein G
GA1055
BosterBio
1ml
EUR 206.4
Rabbit Anti-Goat IgG (H+L)-Gold (~10 nm) Conjugate
30219-G10
Alpha Diagnostics
0.20 ml
EUR 242.4
Rabbit Anti-Goat IgG (H+L)-Gold (10 nm) conjugate
30222G
Alpha Diagnostics
0.5 ml
EUR 242.4
Goat anti Mouse IgG (20 nm Gold Colloid)
43R-IG087GD
Fitzgerald
1 ml
EUR 846
Description: Goat anti Mouse IgG secondary antibody (20 nm Gold Colloid)
Goat Anti-Human IgM (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10219
Alpha Diagnostics
500 ul
EUR 300
Protein A colloidal gold conjugated (40 nm, OD10)
CG-PRTA40
Alpha Diagnostics
500 ul
EUR 270
Protein G colloidal gold conjugated (40 nm, OD10)
CG-PRTG40
Alpha Diagnostics
500 ul
EUR 270
Goat anti Rabbit IgG (15 nm Gold Colloid)
43R-IG085GD
Fitzgerald
1 ml
EUR 846
Description: Goat anti Rabbit IgG secondary antibody (15 nm Gold Colloid)
Goat anti Rabbit IgG (20 nm Gold Colloid)
43R-IG086GD
Fitzgerald
1 ml
EUR 878.4
Description: Goat anti Rabbit IgG secondary antibody (20 nm Gold Colloid)
Goat anti Rabbit IgG (5 nm Gold Colloid)
43R-IG100GD
Fitzgerald
1 ml
EUR 846
Description: Goat anti Rabbit IgG secondary antibody (5 nm Gold Colloid)
Mouse monoclonal anti-Human IgG1 (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10121
Alpha Diagnostics
500 ul
EUR 300
Protein A/G colloidal gold conjugated (40 nm, OD10)
CG-PRTAG40
Alpha Diagnostics
500 ul
EUR 270
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RHA-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RHA-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RHA-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RHA-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RHA-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RHA-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RHM-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RHM-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RHM-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RHM-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RHM-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RHM-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RHM-10-980
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RCG-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RCG-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RCG-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RCG-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RCG-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RCG-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RCG-10-980
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RCN-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RCN-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RCN-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RCN-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RCN-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RCN-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RCN-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RCS-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RCS-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RCS-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RCS-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RCS-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RCS-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RCS-10-980
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RGS-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RGS-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RGS-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RGS-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RGS-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RGS-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RGS-10-980
Creative Diagnostics
1 mL
EUR 1226.4
Gold Colloid (10 nm)
62R-GC010
Fitzgerald
100 ml
EUR 560.4
Description: 10 nm Colloidal Gold particle suspension
DiagNano Silica-Coated PEGylated Gold Nanoparticles, 10 nm
PG-10
Creative Diagnostics
10mL
EUR 862.8
DiagNano Gold Nanorods, diameter 10 nm, absorption max 1064 nm
BR-10-1064
Creative Diagnostics
25 mL
EUR 864
DiagNano Gold Nanorods, diameter 10 nm, absorption max 1200 nm
BR-10-1200
Creative Diagnostics
5 mL
EUR 1226.4
DiagNano Gold Nanorods, diameter 10 nm, absorption max 1400 nm
BR-10-1400
Creative Diagnostics
5 mL
EUR 1226.4
DiagNano Gold Nanorods, diameter 10 nm, absorption max 2100 nm
BR-10-2100
Creative Diagnostics
5 mL
EUR 1226.4
DiagNano Gold Nanorods, diameter 10 nm, absorption max 600 nm
BR-10-600
Creative Diagnostics
25 mL
EUR 864
DiagNano Gold Nanorods, diameter 10 nm, absorption max 650 nm
BR-10-650
Creative Diagnostics
25 mL
EUR 864
DiagNano Gold Nanorods, diameter 10 nm, absorption max 700 nm
BR-10-700
Creative Diagnostics
25 mL
EUR 702
DiagNano Gold Nanorods, diameter 10 nm, absorption max 750 nm
BR-10-750
Creative Diagnostics
25 mL
EUR 702
DiagNano Gold Nanorods, diameter 10 nm, absorption max 780 nm
BR-10-780
Creative Diagnostics
25 mL
EUR 702
DiagNano Gold Nanorods, diameter 10 nm, absorption max 808 nm
BR-10-808
Creative Diagnostics
25 mL
EUR 702
DiagNano Gold Nanorods, diameter 10 nm, absorption max 850 nm
BR-10-850
Creative Diagnostics
25 mL
EUR 702
DiagNano Gold Nanorods, diameter 10 nm, absorption max 900 nm
BR-10-900
Creative Diagnostics
25 mL
EUR 702
DiagNano Gold Nanorods, diameter 10 nm, absorption max 950 nm
BR-10-950
Creative Diagnostics
25 mL
EUR 864
DiagNano Protein A conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RCA-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Protein A conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RCA-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Protein A conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RCA-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Protein A conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RCA-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Protein A conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RCA-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Protein A conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RCA-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Protein A conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RCA-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Protein A conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RCA-10-980
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Organic Gold Nanorods, diameter 10 nm, absorption max 1064 nm
OR-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Organic Gold Nanorods, diameter 10 nm, absorption max 700 nm
OR-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Organic Gold Nanorods, diameter 10 nm, absorption max 750 nm
OR-10-750
Creative Diagnostics
1mL
EUR 1226.4
DiagNano Organic Gold Nanorods, diameter 10 nm, absorption max 780 nm
OR-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Organic Gold Nanorods, diameter 10 nm, absorption max 808 nm
OR-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Organic Gold Nanorods, diameter 10 nm, absorption max 850 nm
OR-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Organic Gold Nanorods, diameter 10 nm, absorption max 900 nm
OR-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Organic Gold Nanorods, diameter 10 nm, absorption max 980 nm
OR-10-980
Creative Diagnostics
1 mL
EUR 1226.4
Methods: We developed a mouse monoclonal anti-ESE-1 antibody, verified the specificity, epitope, and used immunohistochemical staining to assess ESE-1 expression in IBC approved records of 957 breast tumor samples.
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