Staphylococcus aureus and other coagulase-positive Staphylococcus spp. bind the Fc region of IgG antibodies through the expression of protein A (SpA). This species has consequently become a source of false positive signals in antibody-based test designed to detect bacteria other targets. In this work, flow cytometry was used to study the effect of a number of factors in SpA single-cell-mediated binding to anti-human IgG antibody, including: tension; heat kills; Overnight storage; growth phase; cell physiology; surface adhesion; and growth in food model systems. Co-staining cells through antibody-stained with a dye permeability, propidium iodide and Calcein Violet AM, the physiological status of cells associated with antibody-mediated binding SpA.

Generally, cells lacking permeabilised esterase activity is not strong antibody binding. Binding of a number of commercially available polyclonal IgG antibodies to non-Staphylococcus spp. also marked. Not all species of SpA-expressing strong showing binding of mouse IgG, and one species is not known to express a strong showing SpA binding. Most SpA-expressing strain rabbit IgG antibody bound to a certain extent, whereas only one strain bound goat IgG. To reduce or eliminate the binding of IgG-mediated SpA, the following products are evaluated as a blocking reagent and applied before staining with primary or secondary antibody: normal rabbit serum, IgG isotype control, goat IgG and commercial FCR blocking reagent.

Only FCR blocking reagent consistently reduce SpA-mediated binding of Staphylococcus spp. for antibodies to other species and can be recommended as a blocking reagent in an immunoassay designed to detect non-Staphylococcus species.Importance This study characterizes a widespread problem, but little studied associated with antibody-based detection of microbes – Staphylococcus Protein A (SpA) -mediated binding of IgG antibodies – and offered a solution: the commercial use FCR blocking reagent.

A common source of false positive signals in the detection of microbes in clinical, food or environmental samples can be removed by following the findings of the authors. Using flow cytometry, the author shows the degree of heterogeneity in this culture SpA-mediated antibody binding and that the level of antibody-mediated binding SpA strain, phase- and matrix dependent growth of food and affected by simulated food processing care and adherence of cells.

In addition, studies SpA-mediated binding of Staphylococcus spp. for antibodies against other bacterial species produce highly nuanced images, leading the authors to recommend testing against multiple strains of S. aureus and S. hyicus all the antibodies to be incorporated into any immunoassay designed to detect non-Staphylococcus spp.

ETS transcription factor ESE-1 / Elf3 an independent prognostic factor of survival in breast cancer patients with HR + HER2 +

Objective: ETS transcription factor ESE-1 has been shown to be important in breast cancer HER2 + and ESE-1 mRNA expression has been shown to associate with the results prognosis in HER2 + subtypes, as well as in ER +, HER2 + patients with luminal B. However, the clinical significance ESE-1 protein expression is still unknown. The purpose of this current exploratory study was to evaluate the prognostic value of ESE-1 protein expression in breast cancer molecular subtypes with particular emphasis on HER2 + hormone receptor positive (HR + HER2 +) and HER2 positive (HER2 + -only) breast cancer patients.

5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
GA1013	BosterBio	1ml	EUR 206.4

Goat Anti-Human IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10319	Alpha Diagnostics	500 ul	EUR 270

Goat anti-rabbit IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-20319	Alpha Diagnostics	500 ul	EUR 270

10 nm Colloidal Gold Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
GA1024	BosterBio	1ml	EUR 206.4

Rabbit Anti-Goat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-30319	Alpha Diagnostics	500 ul	EUR 270

5 nm Colloidal Gold Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
GA1023	BosterBio	1ml	EUR 206.4

Rabbit anti-Goat IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-30219	Alpha Diagnostics	500 ul	EUR 270

10 nm Colloidal Gold Conjugated Protein A
GA1054	BosterBio	1ml	EUR 206.4

10 nm Colloidal Gold Conjugated Protein G
GA1056	BosterBio	1ml	EUR 206.4

Goat Anti-Human IgM (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10219	Alpha Diagnostics	500 ul	EUR 300

Rabbit Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-50119-R	Alpha Diagnostics	500 ul	EUR 270

Rabbit anti-Mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10
CG-40319	Alpha Diagnostics	500 ul	EUR 270

Rabbit Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10119-R	Alpha Diagnostics	500 ul	EUR 270

Mouse monoclonal anti-Human IgG1 (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10121	Alpha Diagnostics	500 ul	EUR 300

5 nm Colloidal Gold Conjugated Protein A
GA1053	BosterBio	1ml	EUR 206.4

5 nm Colloidal Gold Conjugated Protein G
GA1055	BosterBio	1ml	EUR 206.4

Streptavidin colloidal gold conjugated (40 nm, OD10)
CG-ST40	Alpha Diagnostics	500 ul	EUR 270

Protein A colloidal gold conjugated (40 nm, OD10)
CG-PRTA40	Alpha Diagnostics	500 ul	EUR 270

Protein G colloidal gold conjugated (40 nm, OD10)
CG-PRTG40	Alpha Diagnostics	500 ul	EUR 270

Protein A/G colloidal gold conjugated (40 nm, OD10)
CG-PRTAG40	Alpha Diagnostics	500 ul	EUR 270

Colloidal Gold Conjugate Diluent (10X)
CG-CD10	Alpha Diagnostics	10 ml	EUR 270

Goat anti Mouse IgG (10 nm Gold Colloid)
43R-IG084GD	Fitzgerald	1 ml	EUR 846
Description: Goat anti Mouse IgG secondary antibody (10 nm Gold Colloid)

Colloidal Gold (unconjugated) 10 nm, 1 OD
CG-10NM	Alpha Diagnostics	100 ml	EUR 498

Goat anti Mouse IgG (20 nm Gold Colloid)
43R-IG087GD	Fitzgerald	1 ml	EUR 846
Description: Goat anti Mouse IgG secondary antibody (20 nm Gold Colloid)

Colloidal Gold (unconjugated) 20 nm, 1 OD
CG-20NM	Alpha Diagnostics	100 ml	EUR 498

Colloidal Gold (unconjugated) 40 nm, 1 OD
CG-40NM	Alpha Diagnostics	100 ml	EUR 424.8

Cattle and Goat Tuberculosis Antibodies Rapid Test Kit (Colloidal gold)
abx092019-40tests	Abbexa	40 tests	EUR 585.6

Goat anti Rabbit IgG (15 nm Gold Colloid)
43R-IG085GD	Fitzgerald	1 ml	EUR 846
Description: Goat anti Rabbit IgG secondary antibody (15 nm Gold Colloid)

Goat anti Rabbit IgG (20 nm Gold Colloid)
43R-IG086GD	Fitzgerald	1 ml	EUR 878.4
Description: Goat anti Rabbit IgG secondary antibody (20 nm Gold Colloid)

Goat anti Rabbit IgG (5 nm Gold Colloid)
43R-IG100GD	Fitzgerald	1 ml	EUR 846
Description: Goat anti Rabbit IgG secondary antibody (5 nm Gold Colloid)

Goat Foot and Mouth Disease Virus Type O Antibodies Rapid Test Kit (Colloidal gold)
abx092024-50tests	Abbexa	50 tests	EUR 427.2

Colloidal Gold Running Buffer (10X)
CG-RB10	Alpha Diagnostics	10 ml	EUR 270

Rabies Virus Antibodies Rapid Test Kit (Colloidal gold)
abx092033-40tests	Abbexa	40 tests	EUR 427.2

Goat Anti-Mouse IgG (H+L)-Gold (~10 nm) Conjugate
40319-G10	Alpha Diagnostics	0.20 ml	EUR 242.4

Anticyclic citrullinated peptide antibody rapid test (Colloidal Gold)
abx095260-80Units	Abbexa	80 Units	EUR 1579.2

Cattle and Goat Foot and Mouth Disease Virus Type O Antibodies Rapid Test Kit (Colloidal gold)
abx092023-50tests	Abbexa	50 tests	EUR 427.2

Procalcitonin rapid test (Colloidal Gold)
abx095264-80Units	Abbexa	80 Units	EUR 1144.8

Canine Parvovirus Antibodies Rapid Test Kit (Colloidal gold)
abx092032-40tests	Abbexa	40 tests	EUR 427.2

Total Antibody & IgM of Treponema Pallidum rapid test (Colloidal Gold)
abx095262-80Units	Abbexa	80 Units	EUR 1579.2

Pseudorabies Virus Antibodies Rapid Test Kit (Colloidal gold)
abx092026-50tests	Abbexa	50 tests	EUR 385.2

Anti-SARS-CoV-2 Neutralization Antibody Test Kit (Colloidal gold immunoassay)
CP04010	UnScience	25T/kit	Ask for price
Description: The Anti-SARS-CoV-2 Neutralization Antibody Test Kit (Serum/Plasma/Whole blood) is a qualitative membrane-based immunoassay for the detection of SARS-CoV-2 neutralizing antibodies in serum, plasma and whole blood. The sample is dropped into the sample well, and chromatography is performed under the capillary effect. The SARS-CoV-2 neutralizing antibodies in the sample combined with the colloidal gold-labeled SARS-CoV-2 spike protein(SP), then spread to the test area. It is captured by coated SP subunit RBDNTD-CTD, to form a complex and gather in the test area (T line). The quality control area is coated with mouse anti-chicken IgY, and the colloidal goldlabeled chicken IgY is captureed to form a complex and aggregate in hte quality control area (C line). If the C line does not show color, it indicates that the result is invalid, and this sample need to be tested again.

Aflatoxin Rapid Test Kit (Colloidal gold)
abx092054-50tests	Abbexa	50 tests	EUR 477.6

Salbutamol Rapid Test Kit (Colloidal gold)
abx092043-50tests	Abbexa	50 tests	EUR 410.4

Quinolones Rapid Test Kit (Colloidal gold)
abx092046-50tests	Abbexa	50 tests	EUR 644.4

Clenbuterol Rapid Test Kit (Colloidal gold)
abx092040-50tests	Abbexa	50 tests	EUR 326.4

Ractopamine Rapid Test Kit (Colloidal gold)
abx092042-50tests	Abbexa	50 tests	EUR 360

Zearalenone Rapid Test Kit (Colloidal gold)
abx092051-50tests	Abbexa	50 tests	EUR 493.2

Zearalenone Rapid Test Kit (Colloidal gold)
abx092056-50tests	Abbexa	50 tests	EUR 510

Sulfonamides Rapid Test Kit (Colloidal gold)
abx092047-50tests	Abbexa	50 tests	EUR 661.2

Tetracycline Rapid Test Kit (Colloidal gold)
abx092048-50tests	Abbexa	50 tests	EUR 560.4

Avian Influenza Virus Antibodies Rapid Test Kit (Colloidal gold)
abx092028-50tests	Abbexa	50 tests	EUR 552

Porcine Circovirus 2 Antibodies Rapid Test Kit (Colloidal gold)
abx092027-40tests	Abbexa	40 tests	EUR 385.2

Fetal Fibronectin rapid test (Colloidal Gold)
abx095261-80Units	Abbexa	80 Units	EUR 1863.6

Canine Distemper Virus Antibodies Rapid Test Kit (Colloidal gold)
abx092034-40tests	Abbexa	40 tests	EUR 427.2

Deoxynivalenol Rapid Test Kit (Colloidal gold)
abx092052-50tests	Abbexa	50 tests	EUR 560.4

Deoxynivalenol Rapid Test Kit (Colloidal gold)
abx092053-50tests	Abbexa	50 tests	EUR 560.4

Newcastle Disease Virus Antibodies Rapid Test Kit (Colloidal gold)
abx092029-50tests	Abbexa	50 tests	EUR 585.6

Chloramphenicol Rapid Test Kit (Colloidal gold)
abx092045-50tests	Abbexa	50 tests	EUR 427.2

Aflatoxin B1 Rapid Test Kit (Colloidal gold)
abx092055-50tests	Abbexa	50 tests	EUR 477.6

Aflatoxin B1 Rapid Test Kit (Colloidal gold)
abx092050-50tests	Abbexa	50 tests	EUR 444

Avian Influenza H9 Virus Antibodies Rapid Test Kit (Colloidal gold)
abx092030-40tests	Abbexa	40 tests	EUR 552

Avian Influenza H5 Virus Antibodies Rapid Test Kit (Colloidal gold)
abx092031-50tests	Abbexa	50 tests	EUR 585.6

Malachite Green Rapid Test Kit (Colloidal gold)
abx092041-10tests	Abbexa	10 tests	EUR 360

Classical Swine Fever Virus Antibodies Rapid Test Kit (Colloidal gold)
abx092020-50tests	Abbexa	50 tests	EUR 385.2

Avian Influenza Virus Antigen Rapid Test Kit (Colloidal gold)
abx092015-40tests	Abbexa	40 tests	EUR 518.4

Phenylethanolamine A Rapid Test Kit (Colloidal gold)
abx092044-50tests	Abbexa	50 tests	EUR 560.4

Newcastle Disease Virus Antigen Rapid Test Kit (Colloidal gold)
abx092016-40tests	Abbexa	40 tests	EUR 518.4

Peste des Petits Ruminants Virus Antibodies Rapid Test Kit (Colloidal gold)
abx092018-50tests	Abbexa	50 tests	EUR 585.6

Furaltadone Metabolite Rapid Test Kit (Colloidal gold)
abx092035-50tests	Abbexa	50 tests	EUR 644.4

Cardiac Troponin I (cTnI) rapid test (Colloidal Gold)
abx095265-80Units	Abbexa	80 Units	EUR 1579.2

Furazolidone Metabolite Rapid Test Kit (Colloidal gold)
abx092036-50tests	Abbexa	50 tests	EUR 644.4

Nitrofurazone Metabolite Rapid Test Kit (Colloidal gold)
abx092038-50tests	Abbexa	50 tests	EUR 644.4

Avian Influenza H5 Virus Antigen Rapid Test Kit (Colloidal gold)
abx092014-40tests	Abbexa	40 tests	EUR 585.6

Avian Influenza H7 Virus Antigen Rapid Test Kit (Colloidal gold)
abx092017-40tests	Abbexa	40 tests	EUR 518.4

Human Chlamydia Trachomatis Antigen Rapid Test Kit (Colloidal gold)
abx092049-20tests	Abbexa	20 tests	EUR 276

Aminohydantoin Hydrochloride Rapid Test Kit (Colloidal gold)
abx092037-50tests	Abbexa	50 tests	EUR 644.4

Cattle Foot and Mouth Disease Virus Type O Antibodies Rapid Test Kit (Colloidal gold)
abx092025-50tests	Abbexa	50 tests	EUR 427.2

Porcine Foot and Mouth Disease Virus Type O Antibodies Rapid Test Kit (Colloidal gold)
abx092022-50tests	Abbexa	50 tests	EUR 385.2

Heart-fatty acid binding protein rapid test (Colloidal Gold)
abx095266-80Units	Abbexa	80 Units	EUR 1429.2

Gold Colloid (10 nm)
62R-GC010	Fitzgerald	100 ml	EUR 560.4
Description: 10 nm Colloidal Gold particle suspension

Clenbuterol-Ractopamine-Salbutamol Rapid Test Kit (Colloidal gold)
abx092039-40tests	Abbexa	40 tests	EUR 444

Rabbit Anti-Goat IgG (H+L)-Gold (~10 nm) Conjugate
30219-G10	Alpha Diagnostics	0.20 ml	EUR 242.4

Rabbit Anti-Goat IgG (H+L)-Gold (10 nm) conjugate
30222G	Alpha Diagnostics	0.5 ml	EUR 242.4

Porcine Reproductive and Respiratory Syndrome Virus Antibodies Rapid Test Kit (Colloidal gold)
abx092021-50tests	Abbexa	50 tests	EUR 385.2

N-terminal Pro-B-type Natriuretic Peptide rapid test (Colloidal Gold)
abx095267-80Units	Abbexa	80 Units	EUR 1429.2

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RMG-10-1064	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RMG-10-700	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RMG-10-750	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RMG-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RMG-10-808	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RMG-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RMG-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RMG-10-980	Creative Diagnostics	1 mL	EUR 1226.4

Gold Colloid (100 nm)
62R-GC011	Fitzgerald	100 ml	EUR 586.8
Description: 100 nm Colloidal Gold particle suspension

Gold Colloid (200 nm)
62R-GC012	Fitzgerald	100 ml	EUR 586.8
Description: 200 nm Colloidal Gold particle suspension

Gold Colloid (150 nm)
62R-GC015	Fitzgerald	20 ml	EUR 184.8
Description: 150 nm Colloidal Gold particle suspension

Gold Colloid (250 nm)
62R-GC016	Fitzgerald	20 ml	EUR 184.8
Description: 250 nm Colloidal Gold particle suspension

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RHG-10-1064	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RHG-10-750	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RHG-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RHG-10-808	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RHG-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RHG-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RHG-10-980	Creative Diagnostics	1 mL	EUR 1226.4

Colloidal B
RRSP250-D	Atom Scientific	500ml	EUR 7.54

Colloidal B
RRSP250-E	Atom Scientific	1L	EUR 10.8

Colloidal B
RRSP250-F	Atom Scientific	2.5L	EUR 14.05

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RRG-10-1064	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RRG-10-700	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RRG-10-750	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RRG-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RRG-10-808	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RRG-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RRG-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RRG-10-980	Creative Diagnostics	1 mL	EUR 1226.4

Gold Colloid (15 nm)
62R-GC002	Fitzgerald	100 ml	EUR 586.8
Description: 15 nm Colloidal Gold particle suspension

Gold Colloid (20 nm)
62R-GC005	Fitzgerald	100 ml	EUR 586.8
Description: 20 nm Colloidal Gold particle suspension

Gold Colloid (5 nm)
62R-GC007	Fitzgerald	100 ml	EUR 560.4
Description: 5 nm Colloidal Gold particle suspension

Gold Colloid (50 nm)
62R-GC008	Fitzgerald	100 ml	EUR 586.8
Description: 50 nm Colloidal Gold particle suspension

Gold Colloid (60 nm)
62R-GC009	Fitzgerald	100 ml	EUR 560.4
Description: 60 nm Colloidal Gold particle suspension

Gold Colloid (30 nm)
62R-GC013	Fitzgerald	20 ml	EUR 184.8
Description: 30 nm Colloidal Gold particle suspension

Gold Colloid (80 nm)
62R-GC014	Fitzgerald	20 ml	EUR 184.8
Description: 80 nm Colloidal Gold particle suspension

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 25 nm, absorption max 550 nm
RMG-25-550	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 25 nm, absorption max 600 nm
RMG-25-600	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 25 nm, absorption max 650 nm
RMG-25-650	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 25 nm, absorption max 700 nm
RMG-25-700	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 25 nm, absorption max 750 nm
RMG-25-750	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 25 nm, absorption max 780 nm
RMG-25-780	Creative Diagnostics	1 mL	EUR 1226.4

Methods: We developed a mouse monoclonal anti-ESE-1 antibody, verified the specificity, epitope, and used immunohistochemical staining to assess ESE-1 expression in IBC approved records of 957 breast tumor samples.