Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

Background: Cell membranes expressing enzymes such as ADAM (disintegrin and metalloproteinases) superfamily members are considered key catalyst vital cellular functions. To directly measure this enzyme and determine their relationship with certain cells and function, individual-cell membrane-bound enzyme activity tests are needed, but not available.

Methods: We developed two such tests, using fluorescence resonance energy transfer (FRET) peptide substrate (FPS) and flow cytometry. One test measured the natural processing of live-cell fluorescent products FPS and its binding to membrane-bound enzyme-cell individuals. Test measured other treatment of special-bound generation and glutaraldehyde cross FPS, and consequent fluorescent products are coupled to a membrane-bound enzyme-cell individuals.

Results: confocal microscopy-imaging shows that proteolytic processing of FPS selective and labeled occur in the cell membrane of individual cells. New test tool measured increase FPS specifically related fluorescent products in the cell-substrate concentration-, courtesy of temperature and time dependent. Most of the products processed remain relevant FPS fluorescent cells after washing the cells, suggesting they bind to cells expressing membrane enzymes. Assays measure the level higher than the FPS neon related products on the wild-type cells of ADAM10 knockout mouse fibroblasts and in human monocytes than lymphocytes, which correlated with the presence and expression levels of ADAM10 in the cell membrane, respectively.

Furthermore, test enzyme activity can be combined with anti-ADAM10 fluorescent antibody staining to co-label and more direct association enzyme activity and the level of ADAM10 protein in the cell membrane of individual cells. Conclusion: We report two new test for measuring cell membrane-anchored enzyme activity in individual cells, and the potential for its use to directly study the biology of specific protease-expressing cell surface.

Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.
Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

Building a model system to evaluate CAR T cell therapy using dogs with spontaneous diffuse large B-cell lymphoma.

Some rodent and primate preclinical studies have CAR T cells advanced into the clinic. However, there is no single model to accurately reflect the challenge CAR T effective therapy in human cancer patients. To evaluate the effectiveness of CAR T cell next generation that aims to overcome barriers to long-lasting removal of the tumor, we developed a system to evaluate the CAR T cells in dogs with spontaneous cancers.

Here we report the results of this system and a pilot experiment using CAR T cells to treat dogs spread large B-cell lymphoma (DLBCL). We designed and produced CD20-targeting, a second-generation dog CAR T cells to functional evaluation in vitro and in vivo using lentivectors parallel to the human T cell manufacturing CAR. A trial is the first-in-five species of dogs with DLBCL treated with CAR T do.

Canine CAR T cell antigen-specific function and kill CD20 + targets. Circulation CAR T cells were detected post-infusion, however, the induction of anti-mouse antibody dog ​​(CAMA) is associated with a loss of CAR T cells specific selection pressures in tumors were observed following CD20 + CAR T cell therapy, culminating in a runaway antigen and the emergence of CD20-disease. the survival time of patients correlated with the ex vivo expansion of the product.

Goat anti Mouse IgG
40-GM25S 100 ml
EUR 133
Description: Goat anti Mouse IgG secondary antibody
Goat anti Mouse IgG
40-S8301G000-S4 10 ml
EUR 133
Description: Goat anti Mouse IgG secondary antibody
Goat Anti Mouse IgG
E61I00501 100ug
EUR 343
Goat Anti-mouse IgG
STJ15100178 250 µg
EUR 283
Description: Biotinylated goat anti-mouse IgG enables detection of mouse IgG in enzyme immunoassays such as indirect ELISA.
Polyclonal Goat anti-GST α-form
GST-ANTI-1 50 uL
EUR 280
Polyclonal Goat anti-GST μ-form
GST-ANTI-2 50 uL
EUR 280
Polyclonal Goat anti-GST p-form
GST-ANTI-3 50 uL
EUR 280
Goat Immunoglobulin G (IgG) ELISA Kit
DLR-IgG-g-48T 48T
EUR 464
  • Should the Goat Immunoglobulin G (IgG) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Goat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Goat Immunoglobulin G (IgG) ELISA Kit
DLR-IgG-g-96T 96T
EUR 601
  • Should the Goat Immunoglobulin G (IgG) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Goat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Goat Immunoglobulin G (IgG) ELISA Kit
RDR-IgG-g-48Tests 48 Tests
EUR 482
Goat Immunoglobulin G (IgG) ELISA Kit
RDR-IgG-g-96Tests 96 Tests
EUR 667
Goat Anti-Mouse IgG (Fc) IgG, unlabeled
40151-UL 1 mg
EUR 213
Goat anti Mouse IgG + IgM
41R-1063 1 mg
EUR 137
Description: Goat anti Mouse IgG + IgM secondary antibody
Goat anti Mouse IgG + IgM
41R-1064 1 mg
EUR 194
Description: Goat anti Mouse IgG + IgM secondary antibody
Goat anti Mouse IgG (HRP)
43C-CB1569 1 mg
EUR 198
Description: Goat anti Mouse IgG secondary antibody  (HRP)
Goat anti Mouse IgG (FITC)
43R-1359 2 mg
EUR 186
Description: Goat anti Mouse IgG secondary antibody  (FITC)
Goat anti Mouse IgG (rhodamine)
43R-1360 1 mg
EUR 138
Description: Goat anti Mouse IgG secondary antibody  (rhodamine)
Goat Anti-Mouse IgG/HRP
E3GAM001 200ul
EUR 408
Goat Anti Mouse IgG -Biotin
E61I00502 1mg
EUR 499
Goat Anti Mouse IgG-HRP
E61I00503 1mg
EUR 499
Goat Anti Mouse IgG-FITC
E61I00504 1mg
EUR 611
488 Goat-anti-Mouse IgG
GT26000 1 mg
EUR 375
546 Goat-anti-Mouse IgG
GT26003 1 mg
EUR 375
642 Goat-anti-Mouse IgG
GT26005 1 mg
EUR 375
Goat Anti-Mouse IgG (Fc)
GAMG80Fc-0100 100ml
EUR 404.3
  • Goat Anti-Mouse IgG (Fc) is indicated as RUO. Do not use on humans.
Goat Anti-Mouse IgG (Fc)
GAMG80Fc-0500 500ml
EUR 707.2
  • Goat Anti-Mouse IgG (Fc) is indicated as RUO. Do not use on humans.
Goat Anti-Mouse IgG -HRP
LF-SA8001 0.5 ml
EUR 131
Description: Goat polyclonal to mouse IgG
Goat Anti-Mouse IgG -HRP
LF-SA8001A 1.0 ml
EUR 192
Description: Goat polyclonal to mouse IgG
Goat Anti-Mouse IgG-TRITC
LF-SA8001TR 1.0 mg
EUR 287
Description: Goat polyclonal to mouse IgG
Goat Anti-mouse IgG-ALP
STJ15100031 1 ml
EUR 350
Description: This ALP-conjugate enables detection of mouse IgG in enzyme immunoassays such as indirect ELISA and Western blot.
Goat Anti-Mouse IgG (Fc) IgG-Biotin Conjugate
40151-BT 1 ml
EUR 225
Goat Anti-Mouse IgG (Fc) IgG-FITC Conjugate
40151-FITC 1 ml
EUR 225
Goat Anti-Mouse IgG (Fc) IgG-HRP Conjugate
40151-HRP 1 ml
EUR 225
Ibrutinib drug-Human IgG Conjugate
IBT17-IgG 100 ug
EUR 529
Goat anti Human IgG
70C-CR6047GAP 1 mg
EUR 149
Description: Affinity purified Goat anti Human IgG antibody
Rabbit anti Goat IgG
20-S5801R000-VO 10 ml
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Description: Rabbit anti Goat IgG antibody
Goat anti Cat IgG
40C-CB0215 2 mg
EUR 271
Description: Goat anti Cat IgG secondary antibody
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EUR 234
Description: Goat anti Cat IgG secondary antibody
Rabbit anti Goat IgG
40C-CB0536 1.5 mg
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Description: Rabbit anti Goat IgG secondary antibody
Goat anti Rabbit IgG
40C-CB1157 5 mg
EUR 311
Description: Goat anti Rabbit IgG secondary antibody
Goat anti Monkey IgG
40R-IG002 1 mg
EUR 275
Description: Goat anti Monkey IgG secondary antibody
Goat anti Rabbit IgG
41-GR30 10 mg
EUR 277
Description: Goat anti Rabbit IgG secondary antibody
Goat anti Rabbit IgG
41-GR31 5 mg
EUR 162
Description: Goat anti Rabbit IgG secondary antibody
Goat anti Human IgG
41-XG56 1 mg
EUR 101
Description: Goat anti Human IgG secondary antibody
Goat anti Bovine IgG
41C-CB0136 5 mg
EUR 257
Description: Goat anti Bovine IgG secondary antibody
Rabbit anti Goat IgG
41C-CB0529 2 mg
EUR 244
Description: Rabbit anti Goat IgG secondary antibody
Goat anti Human IgG
41C-CB0957 5 mg
EUR 346
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41C-CB0972 2 mg
EUR 264
Description: Goat anti Human IgG secondary antibody
Goat anti Rabbit IgG
41C-CB1143 2 mg
EUR 278
Description: Goat anti Rabbit IgG secondary antibody
Goat anti Rabbit IgG
41C-CB1150 10 mg
EUR 388
Description: Goat anti Rabbit IgG secondary antibody
Goat anti Monkey IgG
41C-CB1605 1 mg
EUR 275
Description: Goat anti Monkey IgG secondary antibody
Goat anti Human IgG
41R-1026 2 mg
EUR 137
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1027 2 mg
EUR 186
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1028 1 mg
EUR 182
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1029 2 mg
EUR 186
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1030 1 mg
EUR 138
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1031 1 mg
EUR 186
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1032 1 mg
EUR 224
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1033 1 mg
EUR 246
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1034 1 mg
EUR 186
Description: Goat anti Human IgG secondary antibody
Goat anti Rabbit IgG
41R-1079 2 mg
EUR 149
Description: Goat anti Rabbit IgG secondary antibody
Donkey anti Goat IgG
41R-ID001 1 mg
EUR 229
Description: Donkey anti Goat IgG secondary antibody
Donkey anti Goat IgG
40-DG10 100 ml
EUR 241
Description: Donkey anti Goat IgG secondary antibody
Donkey anti Goat IgG
40-DG10S 100 ml
EUR 133
Description: Donkey anti Goat IgG secondary antibody
Goat anti Rabbit IgG
40-GR30 100 ml
EUR 241
Description: Goat anti Rabbit IgG secondary antibody
Goat anti Rabbit IgG
40-GR30S 100 ml
EUR 133
Description: Goat anti Rabbit IgG secondary antibody
Goat anti Rat IgG
40-GR38 100 ml
EUR 241
Description: Goat anti Rat IgG secondary antibody
Goat Anti Human IgG
E61I00201 1mg
EUR 274
Rabbit anti Goat IgG
E61I01002 1mg
EUR 343
goat Anti Rabbit IgG
E61I01301 1mg
EUR 274
Goat anti- human IgG
FNab09896 100µg Ask for price
  • Recommended dilution: WB: 1:1000-1:10000
  • Immunogen: human IgG
  • Research Area: Immunology
Description: Antibody raised against Goat human IgG
Goat Anti-human IgG
STJ15100177 250 µg
EUR 283
Description: Biotinylated goat anti-human IgG enables detection of human IgG in enzyme immunoassays such as indirect ELISA.
Anti-Goat IgG antibody
STJ16101225 1 mg
EUR 191
Anti-Goat IgG antibody
STJ16101226 1.2 mg
EUR 229
Anti-Goat IgG antibody
STJ16101227 1.2 mg
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Goat anti Mouse IgG (H + L)
41C-CB1501 2 mg
EUR 216
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (Fab'2)
41C-CJ0207 1 mg
EUR 298
Description: Goat anti Mouse IgG secondary antibody (Fab'2)
Goat anti Mouse IgG (Fab'2)
41C-CJ0213 1 mg
EUR 294
Description: Goat anti Mouse IgG secondary antibody (Fab'2)
Goat anti Mouse IgG (H + L)
41R-1051 2 mg
EUR 149
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1052 2 mg
EUR 137
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1053 2 mg
EUR 149
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1054 2 mg
EUR 194
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1055 2 mg
EUR 309
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1056 1 mg
EUR 153
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1057 1 mg
EUR 186
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1100 2 mg
EUR 219
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (Alk Phos)
43R-1357 1 mg
EUR 224
Description: Goat anti Mouse IgG secondary antibody  (Alk Phos)
Goat anti Mouse IgG Fc (biotin)
43R-1358 2 mg
EUR 224
Description: Goat anti Mouse IgG secondary antibody (biotin)
Goat anti Mouse IgG + IgM (FITC)
43R-1388 1 mg
EUR 186
Description: Goat anti Mouse IgG + IgM secondary antibody (FITC)
Goat anti Mouse IgG + IgM (rhodamine)
43R-1389 1 mg
EUR 186
Description: Goat anti Mouse IgG + IgM secondary antibody (rhodamine)
Goat anti Mouse IgG + IgM (HRP)
43R-IG051hrp 1 mg
EUR 198
Description: Goat anti Mouse IgG + IgM secondary antibody (HRP)
Goat anti Mouse IgG (H + L)
40-GM25 100 ml
EUR 220
Description: Goat anti Mouse IgG secondary antibody (H + L)
Goat Anti-Mouse IgG-Fab, unlabeled
40154-UL 0.5 mg
EUR 213
Goat Anti-Mouse IgG, aff pure
40319 1 mg
EUR 202
Goat Anti-Mouse IgG, Antisera (Bulk)
40319-GAS-BLK 1-10L, bulk Ask for price
Goat Anti-Mouse IgG-HRP conjugate
40320-200 0.5 ml
EUR 141

Changing the manufactured products increased efficiency transduction and tilted toward such memory T cell phenotype CAR dogs. CAR T cell manufacturing dogs lentivector functional use is feasible. CAR effectively challenge comparable to existing T cell therapy, demonstrating its relevance in the future to inform the design of human clinical trials.

Nathaniel

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