Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

Background: Cell membranes expressing enzymes such as ADAM (disintegrin and metalloproteinases) superfamily members are considered key catalyst vital cellular functions. To directly measure this enzyme and determine their relationship with certain cells and function, individual-cell membrane-bound enzyme activity tests are needed, but not available.

Methods: We developed two such tests, using fluorescence resonance energy transfer (FRET) peptide substrate (FPS) and flow cytometry. One test measured the natural processing of live-cell fluorescent products FPS and its binding to membrane-bound enzyme-cell individuals. Test measured other treatment of special-bound generation and glutaraldehyde cross FPS, and consequent fluorescent products are coupled to a membrane-bound enzyme-cell individuals.

Results: confocal microscopy-imaging shows that proteolytic processing of FPS selective and labeled occur in the cell membrane of individual cells. New test tool measured increase FPS specifically related fluorescent products in the cell-substrate concentration-, courtesy of temperature and time dependent. Most of the products processed remain relevant FPS fluorescent cells after washing the cells, suggesting they bind to cells expressing membrane enzymes. Assays measure the level higher than the FPS neon related products on the wild-type cells of ADAM10 knockout mouse fibroblasts and in human monocytes than lymphocytes, which correlated with the presence and expression levels of ADAM10 in the cell membrane, respectively.

Furthermore, test enzyme activity can be combined with anti-ADAM10 fluorescent antibody staining to co-label and more direct association enzyme activity and the level of ADAM10 protein in the cell membrane of individual cells. Conclusion: We report two new test for measuring cell membrane-anchored enzyme activity in individual cells, and the potential for its use to directly study the biology of specific protease-expressing cell surface.

Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.
Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

Building a model system to evaluate CAR T cell therapy using dogs with spontaneous diffuse large B-cell lymphoma.

Some rodent and primate preclinical studies have CAR T cells advanced into the clinic. However, there is no single model to accurately reflect the challenge CAR T effective therapy in human cancer patients. To evaluate the effectiveness of CAR T cell next generation that aims to overcome barriers to long-lasting removal of the tumor, we developed a system to evaluate the CAR T cells in dogs with spontaneous cancers.

Here we report the results of this system and a pilot experiment using CAR T cells to treat dogs spread large B-cell lymphoma (DLBCL). We designed and produced CD20-targeting, a second-generation dog CAR T cells to functional evaluation in vitro and in vivo using lentivectors parallel to the human T cell manufacturing CAR. A trial is the first-in-five species of dogs with DLBCL treated with CAR T do.

Canine CAR T cell antigen-specific function and kill CD20 + targets. Circulation CAR T cells were detected post-infusion, however, the induction of anti-mouse antibody dog ​​(CAMA) is associated with a loss of CAR T cells specific selection pressures in tumors were observed following CD20 + CAR T cell therapy, culminating in a runaway antigen and the emergence of CD20-disease. the survival time of patients correlated with the ex vivo expansion of the product.

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Goat Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-g-48T 48T
EUR 464
Description: A competitive inhibition quantitative ELISA assay kit for detection of Goat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-g-96T 96T
EUR 601
Description: A competitive inhibition quantitative ELISA assay kit for detection of Goat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-g-48Tests 48 Tests
EUR 482

Goat Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-g-96Tests 96 Tests
EUR 667

Goat Anti-Mouse IgG (Fc) IgG, unlabeled

40151-UL 1 mg
EUR 213

Goat Anti-Mouse IgG/HRP

E3GAM001 200ul
EUR 408

Goat Anti Mouse IgG -Biotin

E61I00502 1mg
EUR 499

Goat Anti Mouse IgG-HRP

E61I00503 1mg
EUR 499

Goat Anti Mouse IgG-FITC

E61I00504 1mg
EUR 611

Goat Anti-mouse IgG-ALP

STJ15100031 1 ml
EUR 350
Description: This ALP-conjugate enables detection of mouse IgG in enzyme immunoassays such as indirect ELISA and Western blot.

488 Goat-anti-Mouse IgG

GT26000 1 mg
EUR 375

546 Goat-anti-Mouse IgG

GT26003 1 mg
EUR 375

642 Goat-anti-Mouse IgG

GT26005 1 mg
EUR 375

Goat Anti-Mouse IgG (Fc)

GAMG80Fc-0100 100ml
EUR 404.3

Goat Anti-Mouse IgG (Fc)

GAMG80Fc-0500 500ml
EUR 707.2

Goat Anti-Mouse IgG -HRP

LF-SA8001 0.5 ml
EUR 131
Description: Goat polyclonal to mouse IgG

Goat Anti-Mouse IgG -HRP

LF-SA8001A 1.0 ml
EUR 192
Description: Goat polyclonal to mouse IgG

Goat Anti-Mouse IgG-TRITC

LF-SA8001TR 1.0 mg
EUR 287
Description: Goat polyclonal to mouse IgG

Goat anti Mouse IgG + IgM

41R-1063 1 mg
EUR 137
Description: Goat anti Mouse IgG + IgM secondary antibody

Goat anti Mouse IgG + IgM

41R-1064 1 mg
EUR 194
Description: Goat anti Mouse IgG + IgM secondary antibody

Goat anti Mouse IgG (HRP)

43C-CB1569 1 mg
EUR 198
Description: Goat anti Mouse IgG secondary antibody  (HRP)

Goat anti Mouse IgG (FITC)

43R-1359 2 mg
EUR 186
Description: Goat anti Mouse IgG secondary antibody  (FITC)

Goat anti Mouse IgG (rhodamine)

43R-1360 1 mg
EUR 138
Description: Goat anti Mouse IgG secondary antibody  (rhodamine)

Goat Anti-Mouse IgG (Fc) IgG-Biotin Conjugate

40151-BT 1 ml
EUR 225

Goat Anti-Mouse IgG (Fc) IgG-FITC Conjugate

40151-FITC 1 ml
EUR 225

Goat Anti-Mouse IgG (Fc) IgG-HRP Conjugate

40151-HRP 1 ml
EUR 225

Ibrutinib drug-Human IgG Conjugate

IBT17-IgG 100 ug
EUR 529

Goat Anti Human IgG

E61I00201 1mg
EUR 274

Rabbit anti Goat IgG

E61I01002 1mg
EUR 343

goat Anti Rabbit IgG

E61I01301 1mg
EUR 274

Goat Anti-human IgG

STJ15100177 250 µg
EUR 283
Description: Biotinylated goat anti-human IgG enables detection of human IgG in enzyme immunoassays such as indirect ELISA.

Anti-Goat IgG antibody

STJ16101225 1 mg
EUR 191

Anti-Goat IgG antibody

STJ16101226 1.2 mg
EUR 229

Anti-Goat IgG antibody

STJ16101227 1.2 mg
EUR 282

Goat anti- human IgG

FNab09896 100µg Ask for price
Description: Antibody raised against Goat human IgG

Donkey anti Goat IgG

40-DG10 100 ml
EUR 241
Description: Donkey anti Goat IgG secondary antibody

Donkey anti Goat IgG

40-DG10S 100 ml
EUR 133
Description: Donkey anti Goat IgG secondary antibody

Goat anti Rabbit IgG

40-GR30 100 ml
EUR 241
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG

40-GR30S 100 ml
EUR 133
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rat IgG

40-GR38 100 ml
EUR 241
Description: Goat anti Rat IgG secondary antibody

Goat anti Cat IgG

40C-CB0215 2 mg
EUR 271
Description: Goat anti Cat IgG secondary antibody

Goat anti Cat IgG

40C-CB0229 2 mg
EUR 234
Description: Goat anti Cat IgG secondary antibody

Rabbit anti Goat IgG

40C-CB0536 1.5 mg
EUR 269
Description: Rabbit anti Goat IgG secondary antibody

Goat anti Rabbit IgG

40C-CB1157 5 mg
EUR 311
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Monkey IgG

40R-IG002 1 mg
EUR 275
Description: Goat anti Monkey IgG secondary antibody

Goat anti Rabbit IgG

41-GR30 10 mg
EUR 277
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG

41-GR31 5 mg
EUR 162
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Human IgG

41-XG56 1 mg
EUR 101
Description: Goat anti Human IgG secondary antibody

Goat anti Bovine IgG

41C-CB0136 5 mg
EUR 257
Description: Goat anti Bovine IgG secondary antibody

Rabbit anti Goat IgG

41C-CB0529 2 mg
EUR 244
Description: Rabbit anti Goat IgG secondary antibody

Goat anti Human IgG

41C-CB0957 5 mg
EUR 346
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41C-CB0972 2 mg
EUR 264
Description: Goat anti Human IgG secondary antibody

Goat anti Rabbit IgG

41C-CB1143 2 mg
EUR 278
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG

41C-CB1150 10 mg
EUR 388
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Monkey IgG

41C-CB1605 1 mg
EUR 275
Description: Goat anti Monkey IgG secondary antibody

Goat anti Human IgG

41R-1026 2 mg
EUR 137
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1027 2 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1028 1 mg
EUR 182
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1029 2 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1030 1 mg
EUR 138
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1031 1 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1032 1 mg
EUR 224
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1033 1 mg
EUR 246
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1034 1 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Goat anti Rabbit IgG

41R-1079 2 mg
EUR 149
Description: Goat anti Rabbit IgG secondary antibody

Donkey anti Goat IgG

41R-ID001 1 mg
EUR 229
Description: Donkey anti Goat IgG secondary antibody

Goat anti Human IgG

70C-CR6047GAP 1 mg
EUR 149
Description: Affinity purified Goat anti Human IgG antibody

Rabbit anti Goat IgG

20-S5801R000-VO 10 ml
EUR 133
Description: Rabbit anti Goat IgG antibody

Goat Anti-Mouse IgG (H+L)

AS071 100 ul
EUR 126

Goat Anti-Mouse IgG(h+l)

C020201-100mg 100mg
EUR 6015

Goat Anti-Mouse IgG(h+l)

C020201-1mg 1mg
EUR 185

Goat anti-Mouse IgG(H+L)

E16SPU101-100 100µg
EUR 239

Goat anti-Mouse IgG(H+L)

E16SPU101-1000 1000µg
EUR 434

Goat anti-Mouse IgG(H+L)

E16SPU101-500 500µg
EUR 343

Goat Anti-Mouse IgG(H+L)

E20-90007 300ul
EUR 343

Goat Anti-Mouse IgG Antibody (TRITC)

abx019213-01ml 0.1 ml
EUR 244

Mouse Anti-Goat IgG (Fc) Antibody

abx023336-1mg 1 mg
EUR 370

Goat Anti-Mouse IgG (Fc) Antibody

abx023402-5mg 5 mg
EUR 495

Goat Anti-Mouse IgG (Fc) Antibody

abx023405-2mg 2 mg
EUR 439

Goat Anti-Mouse IgG (Fc) Antibody

abx023407-2mg 2 mg
EUR 411

Goat Anti-Mouse IgG (Fc) Antibody

abx023543-1mg 1 mg
EUR 523

Goat Anti-Mouse IgG Antibody (HRP)

20-abx400001
  • EUR 189.00
  • EUR 112.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg

Goat Anti-Mouse IgG Antibody (Biotin)

20-abx400030
  • EUR 189.00
  • EUR 112.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg

Goat Anti-Mouse IgG Antibody (FITC)

20-abx400031
  • EUR 189.00
  • EUR 112.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg

Goat anti-Mouse IgG Antibody (BGAL)

abx401031-1ml 1 ml
EUR 370

Goat anti-Mouse IgG Antibody (RPE)

abx401034-05mg 0.5 mg
EUR 537

Goat anti-Mouse IgG Fab Antibody

abx401416-1ml 1 ml
EUR 453

Goat anti-Mouse IgG Antibody (RPE)

abx401556-1ml 1 ml
EUR 606

Goat Anti-mouse IgG+IgM-ALP

STJ15100032 1 ml
EUR 350
Description: Goat anti-mouse IgG+IgM-ALP is specific for mouse IgG and IgM H+L-chains and was purified using immunoaffinity chromatography.

Changing the manufactured products increased efficiency transduction and tilted toward such memory T cell phenotype CAR dogs. CAR T cell manufacturing dogs lentivector functional use is feasible. CAR effectively challenge comparable to existing T cell therapy, demonstrating its relevance in the future to inform the design of human clinical trials.

Nathaniel

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