Background: Cell membranes expressing enzymes such as ADAM (disintegrin and metalloproteinases) superfamily members are considered key catalyst vital cellular functions. To directly measure this enzyme and determine their relationship with certain cells and function, individual-cell membrane-bound enzyme activity tests are needed, but not available.
Methods: We developed two such tests, using fluorescence resonance energy transfer (FRET) peptide substrate (FPS) and flow cytometry. One test measured the natural processing of live-cell fluorescent products FPS and its binding to membrane-bound enzyme-cell individuals. Test measured other treatment of special-bound generation and glutaraldehyde cross FPS, and consequent fluorescent products are coupled to a membrane-bound enzyme-cell individuals.
Results: c onfocal microscopy-imaging shows that proteolytic processing of FPS selective and labeled occur in the cell membrane of individual cells. New test tool measured increase FPS specifically related fluorescent products in the cell-substrate concentration-, courtesy of temperature and time dependent. Most of the products processed remain relevant FPS fluorescent cells after washing the cells, suggesting they bind to cells expressing membrane enzymes. Assays measure the level higher than the FPS neon related products on the wild-type cells of ADAM10 knockout mouse fibroblasts and in human monocytes than lymphocytes, which correlated with the presence and expression levels of ADAM10 in the cell membrane, respectively.
Furthermore, test enzyme activity can be combined with anti-ADAM10 fluorescent antibody staining to co-label and more direct association enzyme activity and the level of ADAM10 protein in the cell membrane of individual cells. Conclusion: We report two new test for measuring cell membrane-anchored enzyme activity in individual cells, and the potential for its use to directly study the biology of specific protease-expressing cell surface.
Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.
Building a model system to evaluate CAR T cell therapy using dogs with spontaneous diffuse large B-cell lymphoma.
Some rodent and primate preclinical studies have CAR T cells advanced into the clinic. However, there is no single model to accurately reflect the challenge CAR T effective therapy in human cancer patients. To evaluate the effectiveness of CAR T cell next generation that aims to overcome barriers to long-lasting removal of the tumor, we developed a system to evaluate the CAR T cells in dogs with spontaneous cancers.
Here we report the results of this system and a pilot experiment using CAR T cells to treat dogs spread large B-cell lymphoma (DLBCL). We designed and produced CD20-targeting, a second-generation dog CAR T cells to functional evaluation in vitro and in vivo using lentivectors parallel to the human T cell manufacturing CAR. A trial is the first-in-five species of dogs with DLBCL treated with CAR T do.
Canine CAR T cell antigen-specific function and kill CD20 + targets. Circulation CAR T cells were detected post-infusion, however, the induction of anti-mouse antibody dog (CAMA) is associated with a loss of CAR T cells specific selection pressures in tumors were observed following CD20 + CAR T cell therapy, culminating in a runaway antigen and the emergence of CD20-disease. the survival time of patients correlated with the ex vivo expansion of the product.
GOAT ANTI MOUSE IgG
MBS235208-5x1mg
MyBiosource
5x1mg
EUR 925
Goat anti Mouse IgG
MBS539273-20mg
MyBiosource
20mg
EUR 440
Goat anti Mouse IgG
MBS539273-5x20mg
MyBiosource
5x20mg
EUR 1820
Goat Anti Mouse IgG
MBS8503086-01mg
MyBiosource
0.1mg
EUR 305
Goat Anti Mouse IgG
MBS8503086-5x01mg
MyBiosource
5x0.1mg
EUR 1220
Goat anti Mouse IgG (Fc)
MBS315381-2mg
MyBiosource
2mg
EUR 315
Goat anti Mouse IgG (Fc)
MBS315381-5x2mg
MyBiosource
5x2mg
EUR 1240
Goat anti Mouse IgG (Fc)
MBS316344-5mg
MyBiosource
5mg
EUR 440
Goat anti Mouse IgG (Fc)
MBS316344-5x5mg
MyBiosource
5x5mg
EUR 1800
Goat anti Mouse IgG (Fc)
MBS316504-2mL
MyBiosource
2mL
EUR 590
Goat anti Mouse IgG (Fc)
MBS316504-5x2mL
MyBiosource
5x2mL
EUR 2485
Goat anti Mouse IgG (Fc)
MBS316505-2mg
MyBiosource
2mg
EUR 505
Goat anti Mouse IgG (Fc)
MBS316505-5x2mg
MyBiosource
5x2mg
EUR 2100
Goat anti Mouse IgG (Fc)
MBS316557-1mg
MyBiosource
1mg
EUR 490
Goat anti Mouse IgG (Fc)
MBS316557-5x1mg
MyBiosource
5x1mg
EUR 2030
GOAT ANTI MOUSE IgG (Fc)
MBS235248-1mg
MyBiosource
1mg
EUR 265
GOAT ANTI MOUSE IgG (Fc)
MBS235248-5x1mg
MyBiosource
5x1mg
EUR 1020
Goat anti Mouse IgG (HRP)
43C-CB1569
Fitzgerald
1 mg
EUR 181
Description: Goat anti Mouse IgG secondary antibody (HRP)
Goat anti Mouse IgG + IgM
41R-1063
Fitzgerald
1 mg
EUR 115
Description: Goat anti Mouse IgG + IgM secondary antibody
Goat anti Mouse IgG + IgM
41R-1064
Fitzgerald
1 mg
EUR 176
Description: Goat anti Mouse IgG + IgM secondary antibody
Goat anti Mouse IgG Fab
MBS571371-1mL
MyBiosource
1mL
EUR 260
Goat anti Mouse IgG Fab
MBS571371-5x1mL
MyBiosource
5x1mL
EUR 1015
Goat anti Mouse IgG Fab
MBS571162-10mg
MyBiosource
10mg
EUR 625
Goat anti Mouse IgG Fab
MBS571162-5x10mg
MyBiosource
5x10mg
EUR 2665
Goat anti Mouse IgG (HRP)
MBS539860-1mg
MyBiosource
1mg
EUR 300
Goat anti Mouse IgG (HRP)
MBS539860-5x1mg
MyBiosource
5x1mg
EUR 1200
Goat anti Mouse IgG (FITC)
43R-1359
Fitzgerald
2 mg
EUR 167
Description: Goat anti Mouse IgG secondary antibody (FITC)
Goat Anti Mouse IgG: FITC
MBS225563-1mg
MyBiosource
1mg
EUR 265
Goat Anti Mouse IgG: FITC
MBS225563-5x1mg
MyBiosource
5x1mg
EUR 1020
Goat Anti Mouse IgG-HRP
E61I00503
EnoGene
1mg
EUR 345
Goat anti Mouse IgG (gamma)
MBS315382-2mg
MyBiosource
2mg
EUR 290
Goat anti Mouse IgG (gamma)
MBS315382-5x2mg
MyBiosource
5x2mg
EUR 1135
Goat Anti Mouse IgG-HRP
MBS8502251-1mg
MyBiosource
1mg
EUR 425
Goat Anti Mouse IgG-HRP
MBS8502251-5x1mg
MyBiosource
5x1mg
EUR 1755
Goat Anti Mouse IgG -Biotin
E61I00502
EnoGene
1mg
EUR 345
Goat Anti Mouse IgG-FITC
E61I00504
EnoGene
1mg
EUR 431.25
Goat Anti Mouse IgG-FITC
MBS8503746-1mg
MyBiosource
1mg
EUR 510
Goat Anti Mouse IgG-FITC
MBS8503746-5x1mg
MyBiosource
5x1mg
EUR 2140
Goat Anti Mouse IgG-Biotin
MBS8503038-5x1mg
MyBiosource
5x1mg
EUR 1755
Goat Anti Mouse IgG-Biotin
MBS8503038-1mg
MyBiosource
1mg
EUR 425
Goat anti Mouse IgG (rhodamine)
43R-1360
Fitzgerald
1 mg
EUR 116
Description: Goat anti Mouse IgG secondary antibody (rhodamine)
Goat anti Mouse IgG (H + L)
41C-CB1501
Fitzgerald
2 mg
EUR 193
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1051
Fitzgerald
2 mg
EUR 127
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1052
Fitzgerald
2 mg
EUR 115
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1053
Fitzgerald
2 mg
EUR 128
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1054
Fitzgerald
2 mg
EUR 176
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1055
Fitzgerald
2 mg
EUR 299
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1056
Fitzgerald
1 mg
EUR 133
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1057
Fitzgerald
1 mg
EUR 167
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
41R-1100
Fitzgerald
2 mg
EUR 176
Description: Goat anti Mouse IgG (H + L) secondary antibody
Goat anti Mouse IgG (H + L)
40-GM25
Fitzgerald
100 ml
EUR 399
Description: Goat anti Mouse IgG secondary antibody (H + L)
Goat anti- mouse IgG(H+L)
FNab09848
FN Test
100µg
EUR 658.5
Description: Antibody raised against Goat mouse IgG(H+L)
Goat anti Mouse IgG (H&L)
MBS315376-2mg
MyBiosource
2mg
EUR 265
Goat anti Mouse IgG (H&L)
MBS315376-5x2mg
MyBiosource
5x2mg
EUR 1020
Goat anti Mouse IgG (H&L)
MBS315378-2mg
MyBiosource
2mg
EUR 265
Goat anti Mouse IgG (H&L)
MBS315378-5x2mg
MyBiosource
5x2mg
EUR 1020
Goat anti Mouse IgG (H&L)
MBS315379-1mg
MyBiosource
1mg
EUR 265
Goat anti Mouse IgG (H&L)
MBS315379-5x1mg
MyBiosource
5x1mg
EUR 1020
Goat anti Mouse IgG (H&L)
MBS316345-5mg
MyBiosource
5mg
EUR 280
Goat anti Mouse IgG (H&L)
MBS316345-5x5mg
MyBiosource
5x5mg
EUR 1090
Goat anti Mouse IgG (H&L)
MBS316442-15mg
MyBiosource
1.5mg
EUR 470
Goat anti Mouse IgG (H&L)
MBS316442-5x15mg
MyBiosource
5x1.5mg
EUR 1935
Goat anti Mouse IgG (H&L)
MBS316751-1mg
MyBiosource
1mg
EUR 310
Goat anti Mouse IgG (H&L)
MBS316751-5x1mg
MyBiosource
5x1mg
EUR 1230
GOAT ANTI MOUSE IGG H&L
MBS316824-5mg
MyBiosource
5mg
EUR 440
GOAT ANTI MOUSE IGG H&L
MBS316824-5x5mg
MyBiosource
5x5mg
EUR 1800
GOAT ANTI MOUSE IgG (H/L)
MBS235231-2mg
MyBiosource
2mg
EUR 440
GOAT ANTI MOUSE IgG (H/L)
MBS235231-5x2mg
MyBiosource
5x2mg
EUR 1815
Goat anti Mouse IgG (H + L)
MBS539137-100mL
MyBiosource
100mL
EUR 870
Goat anti Mouse IgG (H + L)
MBS539137-5x100mL
MyBiosource
5x100mL
EUR 3760
Goat anti Mouse IgG (H + L)
MBS539856-2mg
MyBiosource
2mg
EUR 370
Goat anti Mouse IgG (H + L)
MBS539856-5x2mg
MyBiosource
5x2mg
EUR 1525
Goat anti Mouse IgG (Fab'2)
41C-CJ0207
Fitzgerald
1 mg
EUR 279
Description: Goat anti Mouse IgG secondary antibody (Fab'2)
Goat anti Mouse IgG (Fab'2)
41C-CJ0213
Fitzgerald
1 mg
EUR 333
Description: Goat anti Mouse IgG secondary antibody (Fab'2)
GOAT ANTI MOUSE IgG (Fc):HRP
MBS235188-1mg
MyBiosource
1mg
EUR 320
GOAT ANTI MOUSE IgG (Fc):HRP
MBS235188-5x1mg
MyBiosource
5x1mg
EUR 1255
Goat anti Mouse IgG (Fab'2)
MBS539801-1mg
MyBiosource
1mg
EUR 700
Goat anti Mouse IgG (Fab'2)
MBS539801-5x1mg
MyBiosource
5x1mg
EUR 2990
Goat anti Mouse IgG (Fab'2)
MBS536022-1mg
MyBiosource
1mg
EUR 575
Goat anti Mouse IgG (Fab'2)
MBS536022-5x1mg
MyBiosource
5x1mg
EUR 2435
Goat anti Mouse IgG + IgM (HRP)
43R-IG051hrp
Fitzgerald
1 mg
EUR 181
Description: Goat anti Mouse IgG + IgM secondary antibody (HRP)
GOAT ANTI MOUSE IgG (Fc):FITC
MBS235143-1mg
MyBiosource
1mg
EUR 290
GOAT ANTI MOUSE IgG (Fc):FITC
MBS235143-5x1mg
MyBiosource
5x1mg
EUR 1135
Goat anti Mouse IgG + IgM (HRP)
MBS539229-1mg
MyBiosource
1mg
EUR 300
Goat anti Mouse IgG + IgM (HRP)
MBS539229-5x1mg
MyBiosource
5x1mg
EUR 1200
Goat anti Mouse IgG (Alk Phos)
43R-1357
Fitzgerald
1 mg
EUR 208
Description: Goat anti Mouse IgG secondary antibody (Alk Phos)
Goat anti Mouse IgG + IgM (FITC)
43R-1388
Fitzgerald
1 mg
EUR 167
Description: Goat anti Mouse IgG + IgM secondary antibody (FITC)
Goat anti Mouse IgG Fc (biotin)
43R-1358
Fitzgerald
2 mg
EUR 208
Description: Goat anti Mouse IgG secondary antibody (biotin)
GOAT ANTI MOUSE IgG (RAT ADSORBED)
MBS235255-05mg
MyBiosource
0.5mg
EUR 445
GOAT ANTI MOUSE IgG (RAT ADSORBED)
MBS235255-5x05mg
MyBiosource
5x0.5mg
EUR 1830
Goat anti Mouse IgG + IgM (rhodamine)
43R-1389
Fitzgerald
1 mg
EUR 167
Description: Goat anti Mouse IgG + IgM secondary antibody (rhodamine)
IgG Antibody (Goat anti Mouse) (Fc)
GWB-B6FACE
GenWay Biotech
2 ml
Ask for price
IgG Antibody (Goat anti Mouse) (Fc)
GWB-CD3640
GenWay Biotech
2 ml
Ask for price
IgG Antibody (Goat anti Mouse) (Fc)
GWB-BEA930
GenWay Biotech
1 mg
Ask for price
IgG Antibody (Goat anti Mouse) (Fc)
GWB-D17EA6
GenWay Biotech
2 mg
Ask for price
IgG Antibody (Goat anti Mouse) (Fc)
GWB-D6570C
GenWay Biotech
1 mg
Ask for price
IgG Antibody (Goat anti Mouse) (Fc)
GWB-D8C9F1
GenWay Biotech
2 mg
Ask for price
IgG Antibody (Goat anti Mouse) (Fc)
GWB-49F3C9
GenWay Biotech
1 ml
Ask for price
IgG Antibody (Goat anti Mouse) (Fc)
GWB-1C6569
GenWay Biotech
5 mg
Ask for price
IgG Antibody (Fab) (Goat Anti Mouse)
GWB-5815F1
GenWay Biotech
2 mg
Ask for price
Goat Anti-Rabbit & Mouse IgG-HRP
MBS435024-01mL
MyBiosource
0.1mL
EUR 180
Goat Anti-Rabbit & Mouse IgG-HRP
MBS435024-1mL
MyBiosource
1mL
EUR 300
Goat Anti-Rabbit & Mouse IgG-HRP
MBS435024-5x1mL
MyBiosource
5x1mL
EUR 1020
GOAT ANTI MOUSE IgG (MULTISPECIES ADSORBED)
MBS235223-1mg
MyBiosource
1mg
EUR 460
GOAT ANTI MOUSE IgG (MULTISPECIES ADSORBED)
MBS235223-5x1mg
MyBiosource
5x1mg
EUR 1890
Goat Anti Mouse IgG, F(ab’)2
FNSA-0030
FN Test
500 uL
EUR 367.92
Description: Goat Anti Mouse IgG, F(ab’)2 secondary antibody
GOAT ANTI RAT IgG (MOUSE ADSORBED)
MBS235254-05mg
MyBiosource
0.5mg
EUR 290
GOAT ANTI RAT IgG (MOUSE ADSORBED)
MBS235254-5x05mg
MyBiosource
5x0.5mg
EUR 1135
Goat anti Mouse IgG (H + L) (HRP)
43-GM30
Fitzgerald
2 mg
EUR 264
Description: Goat anti Mouse IgG (H + L) secondary antibody (HRP)
Goat anti Mouse IgG (Fab'2) (PE)
43C-CJ0212
Fitzgerald
500 µg
EUR 551
Description: Goat anti Mouse IgG secondary antibody (Fab'2) (PE)
Goat anti Mouse IgG (Fab'2) (PE)
43C-CJ0218
Fitzgerald
500 µg
EUR 551
Description: Goat anti Mouse IgG secondary antibody (Fab'2) (PE)
Goat anti Mouse IgG (H + L) (HRP)
43R-1318
Fitzgerald
1 mg
EUR 117
Description: Goat anti Mouse IgG (H + L) secondary antibody (HRP)
Goat anti Mouse IgG (H + L) (HRP)
43R-1324
Fitzgerald
1 mg
EUR 116
Description: Goat anti Mouse IgG (H + L) secondary antibody (HRP)
Goat anti Mouse IgG (H + L) (HRP)
43R-1330
Fitzgerald
1 mg
EUR 128
Description: Goat anti Mouse IgG (H + L) secondary antibody (HRP)
Goat anti Mouse IgG (H + L) (HRP)
43R-1336
Fitzgerald
1 mg
EUR 190
Description: Goat anti Mouse IgG (H + L) secondary antibody (HRP)
Goat anti Mouse IgG (H + L) (HRP)
43R-1342
Fitzgerald
1 mg
EUR 336
Description: Goat anti Mouse IgG (H + L) secondary antibody (HRP)
Goat anti Mouse IgG (H + L) (HRP)
43R-1348
Fitzgerald
500 ug
EUR 151
Description: Goat anti Mouse IgG (H + L) secondary antibody (HRP)
Goat anti Mouse IgG (H + L) (HRP)
43R-1354
Fitzgerald
500 ug
EUR 190
Description: Goat anti Mouse IgG (H + L) secondary antibody (HRP)
Goat anti Mouse IgG (H + L) (HRP)
43R-1610
Fitzgerald
1 mg
EUR 192
Description: Goat anti Mouse IgG (H + L) secondary antibody (HRP)
Goat anti Mouse IgG + IgM (H + L)
40C-CB1569
Fitzgerald
1 mg
EUR 196
Description: Goat anti Mouse IgG + IgM (H + L) secondary antibody
GOAT ANTI MOUSE IgG (H/L):HRP
MBS235171-1mg
MyBiosource
1mg
EUR 300
GOAT ANTI MOUSE IgG (H/L):HRP
MBS235171-5x1mg
MyBiosource
5x1mg
EUR 1175
GOAT ANTI MOUSE IgG (H/L):HRP
MBS235172-2mg
MyBiosource
2mg
EUR 360
GOAT ANTI MOUSE IgG (H/L):HRP
MBS235172-5x2mg
MyBiosource
5x2mg
EUR 1445
GOAT ANTI MOUSE IgG (H/L):HRP
MBS219959-2mL
MyBiosource
2mL
EUR 645
GOAT ANTI MOUSE IgG (H/L):HRP
MBS219959-5x2mL
MyBiosource
5x2mL
EUR 2720
Goat anti Mouse IgG (Fab'2) (PE)
MBS538836-05mg
MyBiosource
0.5mg
EUR 1025
Goat anti Mouse IgG (Fab'2) (PE)
MBS538836-5x05mg
MyBiosource
5x0.5mg
EUR 4460
Goat anti Mouse IgG (Fab'2) (PE)
MBS538841-05mg
MyBiosource
0.5mg
EUR 1025
Goat anti Mouse IgG (Fab'2) (PE)
MBS538841-5x05mg
MyBiosource
5x0.5mg
EUR 4460
Goat anti Mouse IgG (H + L) (HRP)
MBS539266-2mg
MyBiosource
2mg
EUR 500
Goat anti Mouse IgG (H + L) (HRP)
MBS539266-5x2mg
MyBiosource
5x2mg
EUR 2100
Goat anti Mouse IgG (Fab'2) (HRP)
43C-CJ0211
Fitzgerald
500 µg
EUR 551
Description: Goat anti Mouse IgG secondary antibody (Fab'2) (HRP)
Goat anti Mouse IgG (Fab'2) (HRP)
43C-CJ0217
Fitzgerald
500 µg
EUR 44
Description: Goat anti Mouse IgG secondary antibody (Fab'2) (HRP)
Goat anti Mouse IgG (H + L) (FITC)
43R-1317
Fitzgerald
2 mg
EUR 123
Description: Goat anti Mouse IgG (H + L) secondary antibody (FITC)
Goat anti Mouse IgG (H + L) (FITC)
43R-1323
Fitzgerald
2 mg
EUR 167
Description: Goat anti Mouse IgG (H + L) secondary antibody (FITC)
Goat anti Mouse IgG (H + L) (FITC)
43R-1329
Fitzgerald
2 mg
EUR 189
Description: Goat anti Mouse IgG (H + L) secondary antibody (FITC)
Goat anti Mouse IgG (H + L) (FITC)
43R-1335
Fitzgerald
1 mg
EUR 167
Description: Goat anti Mouse IgG (H + L) secondary antibody (FITC)
Goat anti Mouse IgG (H + L) (FITC)
43R-1347
Fitzgerald
500 ug
EUR 133
Description: Goat anti Mouse IgG (H + L) secondary antibody (FITC)
Goat anti Mouse IgG (H + L) (FITC)
43R-1353
Fitzgerald
500 ug
EUR 167
Description: Goat anti Mouse IgG (H + L) secondary antibody (FITC)
Goat anti Mouse IgG (H + L) (FITC)
43R-1611
Fitzgerald
1 mg
EUR 192
Description: Goat anti Mouse IgG (H + L) secondary antibody (FITC)
Changing the manufactured products increased efficiency transduction and tilted toward such memory T cell phenotype CAR dogs. CAR T cell manufacturing dogs lentivector functional use is feasible. CAR effectively challenge comparable to existing T cell therapy, demonstrating its relevance in the future to inform the design of human clinical trials.
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