We aim to establish a sensitive and robust assay for estimation of systemic complement activation at the C3 component of complement in mouse and human plasma samples. In order to capture product activation iC3b and C3dg in the relevant specific and physiological manner we utilized construct that consists of sites iC3b / C3dg binds human complement receptor 2 (CR2) is attached to the Fc-portion of IgG mouse.
This construction C3dg and iC3b binding of both mice and humans. We construct CR2-IgG purified from mouse B cell line supernatant myeloma, J558L-CR2-IgG, with protein G affinity chromatography. CR2-IgG construct used to capture fragments of C3 in microtiter wells and anti-mouse or anti-human antibody-C3 is used to detect bound C3 fragments. Initially we tested the specificity of tests using purified fragment C3. Furthermore, using CR2-based test, we measured a signal of up to three-fold higher in activated rat serum compared to non-activated mouse serum, whereas serum activated from C3 knock-out mice did not give a signal.
We tested in vivo produced samples of the experimental mice; complement activation induced by injecting cobra venom factor or heat collected in C57bl6 mouse IgG, followed by the withdrawal of EDTA blood samples at the points of a different time and measurement iC3b / C3dg. We observed a clear time-dependent differences in the signal between samples with expected high and low complement activation. In addition, with the use of an assay to fragment C3 humans,
we observed that patients with systemic lupus erythematosus (SLE) (n = 144) had levels of iC3b / C3dg significantly higher compared with healthy individuals (n = 144) (p <0, 0001). We present two functional immunoassays, which is able to measure the systemic levels of C3 activation product iC3b and C3dg in mice and humans.
To our knowledge, this is the first test for the use of complement activation that are relevant capture physiological constructs such as CR2. This test will be a relevant tool when investigating a mouse model and human diseases involving the complement system.
Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans
Calcium phosphate nanoneedle gene delivery system based immunotherapy of cancer genetics.
Ovarian cancer has become one of the most common gynecologic cancer with a high mortality rate. However, conventional surgery together with combination chemotherapy is difficult to achieve ideal therapeutic effect. Although genetic immunotherapy is applied to an active immune response against the cancer, the lack of efficient in vivo gene delivery technique is still a constraint in clinical applications. To overcome these problems, minicircle DNA vector encoding a human anti-EpCAM / CD3 bispecific antibody (BsAbEPH) has been built.
In addition, various forms of calcium phosphate (Capo) biomaterials prepared. In particular, the capo-nanoneedle-mediated “perforated cell” transfection technology achieve high levels of gene expression in the peritoneal cavity. In intraperitoneal xenograft model with human ovarian cancer cell line SKOV3, Capo-DNA system nanoneedle / minicircle expressed BsAbEPH resulted in significantly retarded the growth of cancer and mouse extension of life span with limited toxicity.
And the system can be made as an off-the-shelf and products that are easy to use. Therefore, Capo-nanoneedle technology-based non-viral gene delivery will have great potential in clinical application.Correspondingly, human CFTR transgene expression was increased significantly by administration of cyclophosphamide compared with the group without treatment.
Goat Anti-mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-40320
Alpha Diagnostics
500 ul
EUR 270
Goat Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10119
Alpha Diagnostics
500 ul
EUR 270
Goat anti-rabbit IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-20119
Alpha Diagnostics
500 ul
EUR 270
Goat Anti-Human IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10319
Alpha Diagnostics
500 ul
EUR 270
Goat anti-rabbit IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-20319
Alpha Diagnostics
500 ul
EUR 270
Rabbit Anti-Goat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-30319
Alpha Diagnostics
500 ul
EUR 270
Rabbit anti-Goat IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-30219
Alpha Diagnostics
500 ul
EUR 270
10 nm Colloidal Gold Conjugated Protein A
GA1054
BosterBio
1ml
EUR 206.4
10 nm Colloidal Gold Conjugated Protein G
GA1056
BosterBio
1ml
EUR 206.4
Goat Anti-Human IgM (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10219
Alpha Diagnostics
500 ul
EUR 300
Rabbit Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-50119-R
Alpha Diagnostics
500 ul
EUR 270
Rabbit anti-Mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10
CG-40319
Alpha Diagnostics
500 ul
EUR 270
Rabbit Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10119-R
Alpha Diagnostics
500 ul
EUR 270
Mouse monoclonal anti-Human IgG1 (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10121
Alpha Diagnostics
500 ul
EUR 300
Goat Anti-Mouse IgG Secondary Antibody PE Conjugated
L30317
SAB
1.0 ml
EUR 229.2
Goat Anti-Mouse IgG Secondary Antibody AP Conjugated
L3033
SAB
1.0 ml
EUR 142.8
Goat Anti-Mouse IgG Secondary Antibody Cy3 Conjugated
L30311
SAB
1.0 ml
EUR 134.4
Goat Anti-Mouse IgG Secondary Antibody APC Conjugated
L30316
SAB
0.5 ml
EUR 229.2
Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated
L3032
SAB
1.0 ml
EUR 134.4
5 nm Colloidal Gold Conjugated Protein A
GA1053
BosterBio
1ml
EUR 206.4
5 nm Colloidal Gold Conjugated Protein G
GA1055
BosterBio
1ml
EUR 206.4
Goat Anti-Mouse IgG Secondary Antibody FITC Conjugated
L30313
SAB
1.0 ml
EUR 116.4
Goat Anti-Mouse IgG Secondary Antibody AMCA Conjugated
L3035
SAB
1.0 ml
EUR 116.4
Goat Anti-Mouse IgG Secondary Antibody AF790 Conjugated
L30310
SAB
0.5 ml
EUR 151.2
Goat Anti-Mouse IgG Secondary Antibody PerCP Conjugated
L30318
SAB
0.5 ml
EUR 229.2
Goat Anti-Mouse IgG Secondary Antibody AF488 Conjugated
L3036
SAB
1.0 ml
EUR 134.4
Goat Anti-Mouse IgG Secondary Antibody AF594 Conjugated
L3037
SAB
1.0 ml
EUR 134.4
Goat Anti-Mouse IgG Secondary Antibody AF647 Conjugated
L3038
SAB
1.0 ml
EUR 134.4
Goat Anti-Mouse IgG Secondary Antibody AF680 Conjugated
L3039
SAB
0.5 ml
EUR 151.2
Goat Anti-Mouse IgG Secondary Antibody Biotin Conjugated
L3034
SAB
1.0 ml
EUR 124.8
Goat Anti-Human IgG Secondary Antibody PE Conjugated
L30217
SAB
1.0 ml
EUR 229.2
Goat Anti-Human IgG Secondary Antibody AP Conjugated
L3023
SAB
1.0 ml
EUR 142.8
Streptavidin colloidal gold conjugated (40 nm, OD10)
CG-ST40
Alpha Diagnostics
500 ul
EUR 270
Goat Anti-Rabbit IgG Secondary Antibody PE Conjugated
L30117
SAB
1.0 ml
EUR 229.2
Goat Anti-Human IgG Secondary Antibody Cy3 Conjugated
L30211
SAB
1.0 ml
EUR 134.4
Goat Anti-Human IgG Secondary Antibody APC Conjugated
L30216
SAB
0.5 ml
EUR 229.2
Goat Anti-Human IgG Secondary Antibody HRP Conjugated
L3022
SAB
1.0 ml
EUR 134.4
Goat Anti-Rabbit IgG Secondary Antibody AP Conjugated
L3073
SAB
1.0 ml
EUR 142.8
Cy3 Conjugated Goat Anti-Mouse IgG (H+L) secondary antibody
BA1031
BosterBio
0.5ml
EUR 218.4
HRP Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1050-0.5
BosterBio
0.5ml
EUR 175.2
HRP Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1050-1
BosterBio
1ml
EUR 279.6
Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated
L30111
SAB
1.0 ml
EUR 134.4
Goat Anti-Rabbit IgG Secondary Antibody APC Conjugated
L30116
SAB
0.5 ml
EUR 229.2
Goat Anti-Rabbit IgG Secondary Antibody HRP Conjugated
L3012
SAB
1.0 ml
EUR 134.4
Goat Anti-Human IgG Secondary Antibody FITC Conjugated
L30213
SAB
1.0 ml
EUR 116.4
Goat Anti-Human IgG Secondary Antibody AMCA Conjugated
L3025
SAB
1.0 ml
EUR 116.4
FITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1101-0.5
BosterBio
0.5mL
EUR 157.2
FITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1101-1
BosterBio
1mL
EUR 242.4
Protein A colloidal gold conjugated (40 nm, OD10)
CG-PRTA40
Alpha Diagnostics
500 ul
EUR 270
Protein G colloidal gold conjugated (40 nm, OD10)
CG-PRTG40
Alpha Diagnostics
500 ul
EUR 270
TRITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1089-0.5
BosterBio
0.5ml
EUR 224.4
TRITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1089-1
BosterBio
1ml
EUR 400.8
Goat Anti-Rabbit IgG Secondary Antibody FITC Conjugated
L30113
SAB
1.0 ml
EUR 116.4
Goat Anti-Human IgG Secondary Antibody PerCP Conjugated
L30218
SAB
0.5 ml
EUR 229.2
Goat Anti-Human IgG Secondary Antibody AF488 Conjugated
L3026
SAB
1.0 ml
EUR 134.4
Goat Anti-Human IgG Secondary Antibody AF594 Conjugated
L3027
SAB
1.0 ml
EUR 134.4
Goat Anti-Human IgG Secondary Antibody AF647 Conjugated
L3028
SAB
1.0 ml
EUR 134.4
Goat Anti-Rabbit IgG Secondary Antibody AMCA Conjugated
L3065
SAB
1.0 ml
EUR 116.4
Biotin Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1001-0.5
BosterBio
0.5ml
EUR 115.2
Biotin Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1001-1
BosterBio
1ml
EUR 157.2
Goat Anti-Rabbit IgG Secondary Antibody PerCP Conjugated
L30118
SAB
0.5 ml
EUR 229.2
Goat Anti-Rabbit IgG Secondary Antibody AF488 Conjugated
L3016
SAB
1.0 ml
EUR 134.4
Goat Anti-Rabbit IgG Secondary Antibody AF594 Conjugated
L3017
SAB
1.0 ml
EUR 134.4
Goat Anti-Rabbit IgG Secondary Antibody AF647 Conjugated
L3018
SAB
1.0 ml
EUR 134.4
Goat Anti-Human IgG Secondary Antibody Biotin Conjugated
L3024
SAB
1.0 ml
EUR 124.8
Goat Anti-Rabbit IgG Secondary Antibody AF680 Conjugated
L3049
SAB
0.5 ml
EUR 151.2
Goat Anti-Rabbit IgG Secondary Antibody AF790 Conjugated
L30510
SAB
0.5 ml
EUR 151.2
Goat Anti-Rabbit IgG Secondary Antibody Biotin Conjugated
L3014
SAB
1.0 ml
EUR 124.8
Goat Anti-Mouse IgG Secondary Antibody Rhodamine (RRX) Conjugated
L30315
SAB
1.0 ml
EUR 116.4
Goat Anti-Mouse IgG Secondary Antibody Rhodamine (TRITC) Conjugated
L30314
SAB
1.0 ml
EUR 116.4
Protein A/G colloidal gold conjugated (40 nm, OD10)
CG-PRTAG40
Alpha Diagnostics
500 ul
EUR 270
Cy3 Conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody
BA1032
BosterBio
0.5ml
EUR 218.4
HRP Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1054-0.5
BosterBio
0.5ml
EUR 175.2
HRP Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1054-1
BosterBio
1ml
EUR 279.6
FITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1105-0.5
BosterBio
0.5mL
EUR 157.2
FITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1105-1
BosterBio
1mL
EUR 242.4
TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1090-0.5
BosterBio
0.5ml
EUR 224.4
TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1090-1
BosterBio
1ml
EUR 400.8
Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1003-0.5
BosterBio
0.5ml
EUR 115.2
Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1003-1
BosterBio
1ml
EUR 157.2
Goat Anti-Human IgG Secondary Antibody Rhodamine (RRX) Conjugated
L30215
SAB
1.0 ml
EUR 116.4
Goat Anti-Rabbit IgG Secondary Antibody Rhodamine (RRX) Conjugated
L30115
SAB
1.0 ml
EUR 116.4
Goat Anti-Human IgG Secondary Antibody Rhodamine (TRITC) Conjugated
L30214
SAB
1.0 ml
EUR 116.4
Goat Anti-Rabbit IgG Secondary Antibody Rhodamine (TRITC) Conjugated
L30114
SAB
1.0 ml
EUR 116.4
HRP Conjugated Goat Anti-mouse IgM secondary antibody
BA1075-0.25
BosterBio
0.25ml
EUR 175.2
HRP Conjugated Goat Anti-mouse IgM secondary antibody
BA1075-0.5
BosterBio
0.5ml
EUR 279.6
Rabbit anti-Goat IgG Secondary Antibody HRP conjugated
L3042
SAB
1.0 ml
EUR 134.4
Goat anti-Rat IgG Secondary Antibody (Biotin Conjugate)
GARBI01
AssayPro
150 ug
EUR 123.6
Goat anti-Rat IgG Secondary Antibody (Biotin Conjugate)
GARBI02
AssayPro
1 mg
EUR 313.2
Biotin Conjugated Goat Anti-mouse IgM secondary antibody
BA1004-0.25
BosterBio
0.25ml
EUR 279.6
Biotin Conjugated Goat Anti-mouse IgM secondary antibody
BA1004-0.5
BosterBio
0.5ml
EUR 486
HRP Conjugated Goat Anti-human IgA secondary antibody
BA1066-0.25
BosterBio
0.25ml
EUR 157.2
HRP Conjugated Goat Anti-human IgA secondary antibody
BA1066-0.5
BosterBio
0.5ml
EUR 242.4
HRP Conjugated Goat Anti-human IgM secondary antibody
BA1077-0.25
BosterBio
0.25ml
EUR 157.2
HRP Conjugated Goat Anti-human IgM secondary antibody
BA1077-0.5
BosterBio
0.5ml
EUR 242.4
Goat anti-Mouse IgG-Fc Secondary Antibody (Biotin Conjugate)
GAMGBI01
AssayPro
100 ug
EUR 150
Goat anti-Mouse IgG-Fc Secondary Antibody (Biotin Conjugate)
GAMGBI02
AssayPro
400 ug
EUR 313.2
FITC Conjugated Goat Anti-human IgA secondary antibody
BA1112-0.25
BosterBio
0.25ml
EUR 175.2
FITC Conjugated Goat Anti-human IgA secondary antibody
BA1112-0.5
BosterBio
0.5ml
EUR 279.6
FITC Conjugated Goat Anti-human IgM secondary antibody
BA1116-0.25
BosterBio
0.25ml
EUR 175.2
FITC Conjugated Goat Anti-human IgM secondary antibody
BA1116-0.5
BosterBio
0.5ml
EUR 279.6
Goat Anti-Rabbit Secondary Antibody, Biotin Conjugated
A12001
EpiGentek
FITC Conjugated Mouse Anti-human IgG (H+L) secondary antibody
BM2003-0.5
BosterBio
0.5mL
EUR 218.4
FITC Conjugated Mouse Anti-human IgG (H+L) secondary antibody
BM2003-1
BosterBio
1mL
EUR 364.8
Biotin Conjugated Goat Anti-human IgM secondary antibody
BA1022-0.25
BosterBio
0.25ml
EUR 279.6
Biotin Conjugated Goat Anti-human IgM secondary antibody
BA1022-0.5
BosterBio
0.5ml
EUR 486
Biotin Conjugated Mouse Anti-human IgG (H+L) secondary antibody
BM2001-0.5
BosterBio
0.5ml
EUR 182.4
Biotin Conjugated Mouse Anti-human IgG (H+L) secondary antibody
BM2001-1
BosterBio
1ml
EUR 412.8
Goat anti-Guinea Pig IgG Secondary Antibody (Biotin Conjugate)
GAGPBI01
AssayPro
150 ug
EUR 150
Goat anti-Guinea Pig IgG Secondary Antibody (Biotin Conjugate)
GAGPBI02
AssayPro
1 mg
EUR 313.2
Cy3 Conjugated Rabbit Anti-Goat IgG (H+L) secondary antibody
BA1034
BosterBio
0.5ml
EUR 218.4
HRP Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1060-0.5
BosterBio
0.5ml
EUR 175.2
HRP Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1060-1
BosterBio
1ml
EUR 279.6
Biotin Conjugated Mouse Anti-rabbit IgG (H+L) secondary antibody
BM2004-0.5
BosterBio
0.5ml
EUR 182.4
Biotin Conjugated Mouse Anti-rabbit IgG (H+L) secondary antibody
BM2004-1
BosterBio
1ml
EUR 412.8
FITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1110-0.5
BosterBio
0.5mL
EUR 157.2
FITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1110-1
BosterBio
1mL
EUR 242.4
TRITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1091-0.5
BosterBio
0.5ml
EUR 224.4
TRITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1091-1
BosterBio
1ml
EUR 400.8
Biotin Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1006-0.5
BosterBio
0.5ml
EUR 115.2
Biotin Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1006-1
BosterBio
1ml
EUR 157.2
Goat anti-Rabbit IgG-Fc Secondary Antibody (Biotin Conjugate)
GARBBI01
AssayPro
150 ug
EUR 123.6
Goat anti-Rabbit IgG-Fc Secondary Antibody (Biotin Conjugate)
GARBBI02
AssayPro
1 mg
EUR 313.2
Goat anti-Rat IgG Secondary AssayLite Antibody (APC Conjugate)
GARAPC01
AssayPro
150 ug
EUR 513.6
Goat anti-Rat IgG Secondary AssayLite Antibody (RPE Conjugate)
GARRPE01
AssayPro
150 ug
EUR 513.6
Goat anti-Rat IgG Secondary AssayLite Antibody (FITC Conjugate)
GARFITC01
AssayPro
150 ug
EUR 513.6
Goat anti-Rat IgG Secondary AssayLite Antibody (PerCP Conjugate)
GARPERCP01
AssayPro
150 ug
EUR 565.2
Goat anti-Mouse IgG-Fc Secondary AssayLite Antibody (APC Conjugate)
GAMGAPC01
AssayPro
150 ug
EUR 513.6
These data indicate that sustained expression of the human CFTR transgene in the lungs of mice through a delivery vector can be achieved by repeated temporary immunosuppression.
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