Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans

Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans

We aim to establish a sensitive and robust assay for estimation of systemic complement activation at the C3 component of complement in mouse and human plasma samples. In order to capture product activation iC3b and C3dg in the relevant specific and physiological manner we utilized construct that consists of sites iC3b / C3dg binds human complement receptor 2 (CR2) is attached to the Fc-portion of IgG mouse.

This construction C3dg and iC3b binding of both mice and humans. We construct CR2-IgG purified from mouse B cell line supernatant myeloma, J558L-CR2-IgG, with protein G affinity chromatography. CR2-IgG construct used to capture fragments of C3 in microtiter wells and anti-mouse or anti-human antibody-C3 is used to detect bound C3 fragments. Initially we tested the specificity of tests using purified fragment C3. Furthermore, using CR2-based test, we measured a signal of up to three-fold higher in activated rat serum compared to non-activated mouse serum, whereas serum activated from C3 knock-out mice did not give a signal.

We tested in vivo produced samples of the experimental mice; complement activation induced by injecting cobra venom factor or heat collected in C57bl6 mouse IgG, followed by the withdrawal of EDTA blood samples at the points of a different time and measurement iC3b / C3dg. We observed a clear time-dependent differences in the signal between samples with expected high and low complement activation. In addition, with the use of an assay to fragment C3 humans,

we observed that patients with systemic lupus erythematosus (SLE) (n = 144) had levels of iC3b / C3dg significantly higher compared with healthy individuals (n = 144) (p <0, 0001). We present two functional immunoassays, which is able to measure the systemic levels of C3 activation product iC3b and C3dg in mice and humans.

To our knowledge, this is the first test for the use of complement activation that are relevant capture physiological constructs such as CR2. This test will be a relevant tool when investigating a mouse model and human diseases involving the complement system.

Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans
Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans

Calcium phosphate nanoneedle gene delivery system based immunotherapy of cancer genetics.

Ovarian cancer has become one of the most common gynecologic cancer with a high mortality rate. However, conventional surgery together with combination chemotherapy is difficult to achieve ideal therapeutic effect. Although genetic immunotherapy is applied to an active immune response against the cancer, the lack of efficient in vivo gene delivery technique is still a constraint in clinical applications. To overcome these problems, minicircle DNA vector encoding a human anti-EpCAM / CD3 bispecific antibody (BsAbEPH) has been built.

In addition, various forms of calcium phosphate (Capo) biomaterials prepared. In particular, the capo-nanoneedle-mediated “perforated cell” transfection technology achieve high levels of gene expression in the peritoneal cavity. In intraperitoneal xenograft model with human ovarian cancer cell line SKOV3, Capo-DNA system nanoneedle / minicircle expressed BsAbEPH resulted in significantly retarded the growth of cancer and mouse extension of life span with limited toxicity.

And the system can be made as an off-the-shelf and products that are easy to use. Therefore, Capo-nanoneedle technology-based non-viral gene delivery will have great potential in clinical application.Correspondingly, human CFTR transgene expression was increased significantly by administration of cyclophosphamide compared with the group without treatment.

10 nm Colloidal Gold Conjugated Protein G

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Goat Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)

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Goat anti-rabbit IgG (Fc) colloidal gold conjugated (40 nm, OD10)

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Rabbit Anti-Goat IgG (Fc) colloidal gold conjugated (40 nm, OD10)

CG-30319 500 ul
EUR 270

Goat Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)

CG-50119 500 ul
EUR 270

Goat anti Mouse IgG (10 nm Gold Colloid)

43R-IG084GD 1 ml
EUR 846
Description: Goat anti Mouse IgG secondary antibody (10 nm Gold Colloid)

Goat Anti-mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10)

CG-40320 500 ul
EUR 270

Goat Anti-Human IgG (H+L) colloidal gold conjugated (40 nm, OD10)

CG-10319 500 ul
EUR 270

Goat anti-rabbit IgG (H+L) colloidal gold conjugated (40 nm, OD10)

CG-20319 500 ul
EUR 270

Rabbit anti-Goat IgG (H+L) colloidal gold conjugated (40 nm, OD10)

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Colloidal Gold (unconjugated) 10 nm, 1 OD

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EUR 498

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm

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DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm

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DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm

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DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm

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DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm

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DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm

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DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm

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DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm

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Goat Anti-Mouse IgG (H+L)-Gold (~10 nm) Conjugate

40319-G10 0.20 ml
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Rabbit anti-Mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10

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Rabbit Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)

CG-10119-R 500 ul
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Rabbit Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)

CG-50119-R 500 ul
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DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm

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EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm

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DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm

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DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm

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EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm

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DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm

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DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm

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EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm

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EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm

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EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm

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EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm

RHG-10-780 1 mL
EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm

RHG-10-808 1 mL
EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm

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EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm

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EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm

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Streptavidin colloidal gold conjugated (40 nm, OD10)

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5 nm Colloidal Gold Conjugated Protein A

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5 nm Colloidal Gold Conjugated Protein G

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Rabbit Anti-Goat IgG (H+L)-Gold (~10 nm) Conjugate

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EUR 242.4

Rabbit Anti-Goat IgG (H+L)-Gold (10 nm) conjugate

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Goat anti Mouse IgG (20 nm Gold Colloid)

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EUR 846
Description: Goat anti Mouse IgG secondary antibody (20 nm Gold Colloid)

Goat Anti-Human IgM (H+L) colloidal gold conjugated (40 nm, OD10)

CG-10219 500 ul
EUR 300

Protein A colloidal gold conjugated (40 nm, OD10)

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Protein G colloidal gold conjugated (40 nm, OD10)

CG-PRTG40 500 ul
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Goat Anti-Mouse IgG Secondary Antibody AF790 Conjugated

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EUR 151.2

Goat Anti-Mouse IgG Secondary Antibody Cy3 Conjugated

L30311 1.0 ml
EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody FITC Conjugated

L30313 1.0 ml
EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody APC Conjugated

L30316 0.5 ml
EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody PE Conjugated

L30317 1.0 ml
EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody PerCP Conjugated

L30318 0.5 ml
EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated

L3032 1.0 ml
EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AP Conjugated

L3033 1.0 ml
EUR 142.8

Goat Anti-Mouse IgG Secondary Antibody Biotin Conjugated

L3034 1.0 ml
EUR 124.8

Goat Anti-Mouse IgG Secondary Antibody AMCA Conjugated

L3035 1.0 ml
EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody AF488 Conjugated

L3036 1.0 ml
EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF594 Conjugated

L3037 1.0 ml
EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF647 Conjugated

L3038 1.0 ml
EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF680 Conjugated

L3039 0.5 ml
EUR 151.2

Goat anti Rabbit IgG (15 nm Gold Colloid)

43R-IG085GD 1 ml
EUR 846
Description: Goat anti Rabbit IgG secondary antibody (15 nm Gold Colloid)

Goat anti Rabbit IgG (20 nm Gold Colloid)

43R-IG086GD 1 ml
EUR 878.4
Description: Goat anti Rabbit IgG secondary antibody (20 nm Gold Colloid)

Goat anti Rabbit IgG (5 nm Gold Colloid)

43R-IG100GD 1 ml
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Description: Goat anti Rabbit IgG secondary antibody (5 nm Gold Colloid)

DiagNano Silica-Coated Gold Nanoparticles, 10 nm

CG-10 10mL
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DiagNano Fluorophore Labeled Gold Nanoparticles, 10 nm

GFL-10 1 mL
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DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm

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DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm

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DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm

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DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm

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DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm

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DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm

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DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm

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DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm

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DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm

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DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm

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DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm

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Mouse monoclonal anti-Human IgG1 (Fc) colloidal gold conjugated (40 nm, OD10)

CG-10121 500 ul
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Protein A/G colloidal gold conjugated (40 nm, OD10)

CG-PRTAG40 500 ul
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Colloidal Gold (unconjugated) 20 nm, 1 OD

CG-20NM 100 ml
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Colloidal Gold (unconjugated) 40 nm, 1 OD

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DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm

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DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm

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DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm

RCG-10-780 1 mL
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DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm

RCG-10-808 1 mL
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DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm

RCG-10-850 1 mL
EUR 1226.4

DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm

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DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm

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DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm

RCN-10-1064 1 mL
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DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm

RCN-10-700 1 mL
EUR 1226.4

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm

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DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm

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DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm

RCN-10-808 1 mL
EUR 1226.4

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm

RCN-10-850 1 mL
EUR 1226.4

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm

RCN-10-900 1 mL
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DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm

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DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm

RCS-10-700 1 mL
EUR 1226.4

DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm

RCS-10-750 1 mL
EUR 1226.4

DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm

RCS-10-780 1 mL
EUR 1226.4

DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm

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EUR 1226.4

DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm

RCS-10-900 1 mL
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DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm

RCS-10-980 1 mL
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DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm

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DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm

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DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm

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DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm

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EUR 1226.4

DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm

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DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm

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Gold Colloid (10 nm)

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EUR 560.4
Description: 10 nm Colloidal Gold particle suspension

Goat Anti-Mouse IgG Secondary Antibody Rhodamine (TRITC) Conjugated

L30314 1.0 ml
EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody Rhodamine (RRX) Conjugated

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EUR 116.4

DiagNano Silica-Coated PEGylated Gold Nanoparticles, 10 nm

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EUR 862.8

DiagNano Gold Nanorods, diameter 10 nm, absorption max 1064 nm

BR-10-1064 25 mL
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DiagNano Gold Nanorods, diameter 10 nm, absorption max 1200 nm

BR-10-1200 5 mL
EUR 1226.4

DiagNano Gold Nanorods, diameter 10 nm, absorption max 1400 nm

BR-10-1400 5 mL
EUR 1226.4

DiagNano Gold Nanorods, diameter 10 nm, absorption max 2100 nm

BR-10-2100 5 mL
EUR 1226.4

DiagNano Gold Nanorods, diameter 10 nm, absorption max 600 nm

BR-10-600 25 mL
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DiagNano Gold Nanorods, diameter 10 nm, absorption max 650 nm

BR-10-650 25 mL
EUR 864

DiagNano Gold Nanorods, diameter 10 nm, absorption max 700 nm

BR-10-700 25 mL
EUR 702

DiagNano Gold Nanorods, diameter 10 nm, absorption max 750 nm

BR-10-750 25 mL
EUR 702

DiagNano Gold Nanorods, diameter 10 nm, absorption max 780 nm

BR-10-780 25 mL
EUR 702

DiagNano Gold Nanorods, diameter 10 nm, absorption max 808 nm

BR-10-808 25 mL
EUR 702

DiagNano Gold Nanorods, diameter 10 nm, absorption max 850 nm

BR-10-850 25 mL
EUR 702

DiagNano Gold Nanorods, diameter 10 nm, absorption max 900 nm

BR-10-900 25 mL
EUR 702

DiagNano Gold Nanorods, diameter 10 nm, absorption max 950 nm

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EUR 864

Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated

L30111 1.0 ml
EUR 134.4

These data indicate that sustained expression of the human CFTR transgene in the lungs of mice through a delivery vector can be achieved by repeated temporary immunosuppression.

Nathaniel

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