Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans

Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans

We aim to establish a sensitive and robust assay for estimation of systemic complement activation at the C3 component of complement in mouse and human plasma samples. In order to capture product activation iC3b and C3dg in the relevant specific and physiological manner we utilized construct that consists of sites iC3b / C3dg binds human complement receptor 2 (CR2) is attached to the Fc-portion of IgG mouse.

This construction C3dg and iC3b binding of both mice and humans. We construct CR2-IgG purified from mouse B cell line supernatant myeloma, J558L-CR2-IgG, with protein G affinity chromatography. CR2-IgG construct used to capture fragments of C3 in microtiter wells and anti-mouse or anti-human antibody-C3 is used to detect bound C3 fragments. Initially we tested the specificity of tests using purified fragment C3. Furthermore, using CR2-based test, we measured a signal of up to three-fold higher in activated rat serum compared to non-activated mouse serum, whereas serum activated from C3 knock-out mice did not give a signal.

We tested in vivo produced samples of the experimental mice; complement activation induced by injecting cobra venom factor or heat collected in C57bl6 mouse IgG, followed by the withdrawal of EDTA blood samples at the points of a different time and measurement iC3b / C3dg. We observed a clear time-dependent differences in the signal between samples with expected high and low complement activation. In addition, with the use of an assay to fragment C3 humans,

we observed that patients with systemic lupus erythematosus (SLE) (n = 144) had levels of iC3b / C3dg significantly higher compared with healthy individuals (n = 144) (p <0, 0001). We present two functional immunoassays, which is able to measure the systemic levels of C3 activation product iC3b and C3dg in mice and humans.

To our knowledge, this is the first test for the use of complement activation that are relevant capture physiological constructs such as CR2. This test will be a relevant tool when investigating a mouse model and human diseases involving the complement system.

Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans
Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans

Calcium phosphate nanoneedle gene delivery system based immunotherapy of cancer genetics.

Ovarian cancer has become one of the most common gynecologic cancer with a high mortality rate. However, conventional surgery together with combination chemotherapy is difficult to achieve ideal therapeutic effect. Although genetic immunotherapy is applied to an active immune response against the cancer, the lack of efficient in vivo gene delivery technique is still a constraint in clinical applications. To overcome these problems, minicircle DNA vector encoding a human anti-EpCAM / CD3 bispecific antibody (BsAbEPH) has been built.

In addition, various forms of calcium phosphate (Capo) biomaterials prepared. In particular, the capo-nanoneedle-mediated “perforated cell” transfection technology achieve high levels of gene expression in the peritoneal cavity. In intraperitoneal xenograft model with human ovarian cancer cell line SKOV3, Capo-DNA system nanoneedle / minicircle expressed BsAbEPH resulted in significantly retarded the growth of cancer and mouse extension of life span with limited toxicity.

And the system can be made as an off-the-shelf and products that are easy to use. Therefore, Capo-nanoneedle technology-based non-viral gene delivery will have great potential in clinical application.Correspondingly, human CFTR transgene expression was increased significantly by administration of cyclophosphamide compared with the group without treatment.

Goat Anti-mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10)

CG-40320 500 ul
EUR 270

Goat Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)

CG-10119 500 ul
EUR 270

Goat anti-rabbit IgG (Fc) colloidal gold conjugated (40 nm, OD10)

CG-20119 500 ul
EUR 270

Goat Anti-Human IgG (H+L) colloidal gold conjugated (40 nm, OD10)

CG-10319 500 ul
EUR 270

Goat anti-rabbit IgG (H+L) colloidal gold conjugated (40 nm, OD10)

CG-20319 500 ul
EUR 270

Rabbit Anti-Goat IgG (Fc) colloidal gold conjugated (40 nm, OD10)

CG-30319 500 ul
EUR 270

Rabbit anti-Goat IgG (H+L) colloidal gold conjugated (40 nm, OD10)

CG-30219 500 ul
EUR 270

10 nm Colloidal Gold Conjugated Protein A

GA1054 1ml
EUR 206.4

10 nm Colloidal Gold Conjugated Protein G

GA1056 1ml
EUR 206.4

Goat Anti-Human IgM (H+L) colloidal gold conjugated (40 nm, OD10)

CG-10219 500 ul
EUR 300

Rabbit Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)

CG-50119-R 500 ul
EUR 270

Rabbit anti-Mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10

CG-40319 500 ul
EUR 270

Rabbit Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)

CG-10119-R 500 ul
EUR 270

Mouse monoclonal anti-Human IgG1 (Fc) colloidal gold conjugated (40 nm, OD10)

CG-10121 500 ul
EUR 300

Goat Anti-Mouse IgG Secondary Antibody PE Conjugated

L30317 1.0 ml
EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody AP Conjugated

L3033 1.0 ml
EUR 142.8

Goat Anti-Mouse IgG Secondary Antibody Cy3 Conjugated

L30311 1.0 ml
EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody APC Conjugated

L30316 0.5 ml
EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated

L3032 1.0 ml
EUR 134.4

5 nm Colloidal Gold Conjugated Protein A

GA1053 1ml
EUR 206.4

5 nm Colloidal Gold Conjugated Protein G

GA1055 1ml
EUR 206.4

Goat Anti-Mouse IgG Secondary Antibody FITC Conjugated

L30313 1.0 ml
EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody AMCA Conjugated

L3035 1.0 ml
EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody AF790 Conjugated

L30310 0.5 ml
EUR 151.2

Goat Anti-Mouse IgG Secondary Antibody PerCP Conjugated

L30318 0.5 ml
EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody AF488 Conjugated

L3036 1.0 ml
EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF594 Conjugated

L3037 1.0 ml
EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF647 Conjugated

L3038 1.0 ml
EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF680 Conjugated

L3039 0.5 ml
EUR 151.2

Goat Anti-Mouse IgG Secondary Antibody Biotin Conjugated

L3034 1.0 ml
EUR 124.8

Goat Anti-Human IgG Secondary Antibody PE Conjugated

L30217 1.0 ml
EUR 229.2

Goat Anti-Human IgG Secondary Antibody AP Conjugated

L3023 1.0 ml
EUR 142.8

Streptavidin colloidal gold conjugated (40 nm, OD10)

CG-ST40 500 ul
EUR 270

Goat Anti-Rabbit IgG Secondary Antibody PE Conjugated

L30117 1.0 ml
EUR 229.2

Goat Anti-Human IgG Secondary Antibody Cy3 Conjugated

L30211 1.0 ml
EUR 134.4

Goat Anti-Human IgG Secondary Antibody APC Conjugated

L30216 0.5 ml
EUR 229.2

Goat Anti-Human IgG Secondary Antibody HRP Conjugated

L3022 1.0 ml
EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody AP Conjugated

L3073 1.0 ml
EUR 142.8

Cy3 Conjugated Goat Anti-Mouse IgG (H+L) secondary antibody

BA1031 0.5ml
EUR 218.4

HRP Conjugated Goat Anti-mouse IgG (H+L) secondary antibody

BA1050-0.5 0.5ml
EUR 175.2

HRP Conjugated Goat Anti-mouse IgG (H+L) secondary antibody

BA1050-1 1ml
EUR 279.6

Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated

L30111 1.0 ml
EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody APC Conjugated

L30116 0.5 ml
EUR 229.2

Goat Anti-Rabbit IgG Secondary Antibody HRP Conjugated

L3012 1.0 ml
EUR 134.4

Goat Anti-Human IgG Secondary Antibody FITC Conjugated

L30213 1.0 ml
EUR 116.4

Goat Anti-Human IgG Secondary Antibody AMCA Conjugated

L3025 1.0 ml
EUR 116.4

FITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody

BA1101-0.5 0.5mL
EUR 157.2

FITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody

BA1101-1 1mL
EUR 242.4

Protein A colloidal gold conjugated (40 nm, OD10)

CG-PRTA40 500 ul
EUR 270

Protein G colloidal gold conjugated (40 nm, OD10)

CG-PRTG40 500 ul
EUR 270

TRITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody

BA1089-0.5 0.5ml
EUR 224.4

TRITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody

BA1089-1 1ml
EUR 400.8

Goat Anti-Rabbit IgG Secondary Antibody FITC Conjugated

L30113 1.0 ml
EUR 116.4

Goat Anti-Human IgG Secondary Antibody PerCP Conjugated

L30218 0.5 ml
EUR 229.2

Goat Anti-Human IgG Secondary Antibody AF488 Conjugated

L3026 1.0 ml
EUR 134.4

Goat Anti-Human IgG Secondary Antibody AF594 Conjugated

L3027 1.0 ml
EUR 134.4

Goat Anti-Human IgG Secondary Antibody AF647 Conjugated

L3028 1.0 ml
EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody AMCA Conjugated

L3065 1.0 ml
EUR 116.4

Biotin Conjugated Goat Anti-mouse IgG (H+L) secondary antibody

BA1001-0.5 0.5ml
EUR 115.2

Biotin Conjugated Goat Anti-mouse IgG (H+L) secondary antibody

BA1001-1 1ml
EUR 157.2

Goat Anti-Rabbit IgG Secondary Antibody PerCP Conjugated

L30118 0.5 ml
EUR 229.2

Goat Anti-Rabbit IgG Secondary Antibody AF488 Conjugated

L3016 1.0 ml
EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody AF594 Conjugated

L3017 1.0 ml
EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody AF647 Conjugated

L3018 1.0 ml
EUR 134.4

Goat Anti-Human IgG Secondary Antibody Biotin Conjugated

L3024 1.0 ml
EUR 124.8

Goat Anti-Rabbit IgG Secondary Antibody AF680 Conjugated

L3049 0.5 ml
EUR 151.2

Goat Anti-Rabbit IgG Secondary Antibody AF790 Conjugated

L30510 0.5 ml
EUR 151.2

Goat Anti-Rabbit IgG Secondary Antibody Biotin Conjugated

L3014 1.0 ml
EUR 124.8

Goat Anti-Mouse IgG Secondary Antibody Rhodamine (RRX) Conjugated

L30315 1.0 ml
EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody Rhodamine (TRITC) Conjugated

L30314 1.0 ml
EUR 116.4

Protein A/G colloidal gold conjugated (40 nm, OD10)

CG-PRTAG40 500 ul
EUR 270

Cy3 Conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody

BA1032 0.5ml
EUR 218.4

HRP Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1054-0.5 0.5ml
EUR 175.2

HRP Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1054-1 1ml
EUR 279.6

FITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1105-0.5 0.5mL
EUR 157.2

FITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1105-1 1mL
EUR 242.4

TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1090-0.5 0.5ml
EUR 224.4

TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1090-1 1ml
EUR 400.8

Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1003-0.5 0.5ml
EUR 115.2

Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody

BA1003-1 1ml
EUR 157.2

Goat Anti-Human IgG Secondary Antibody Rhodamine (RRX) Conjugated

L30215 1.0 ml
EUR 116.4

Goat Anti-Rabbit IgG Secondary Antibody Rhodamine (RRX) Conjugated

L30115 1.0 ml
EUR 116.4

Goat Anti-Human IgG Secondary Antibody Rhodamine (TRITC) Conjugated

L30214 1.0 ml
EUR 116.4

Goat Anti-Rabbit IgG Secondary Antibody Rhodamine (TRITC) Conjugated

L30114 1.0 ml
EUR 116.4

HRP Conjugated Goat Anti-mouse IgM secondary antibody

BA1075-0.25 0.25ml
EUR 175.2

HRP Conjugated Goat Anti-mouse IgM secondary antibody

BA1075-0.5 0.5ml
EUR 279.6

Rabbit anti-Goat IgG Secondary Antibody HRP conjugated

L3042 1.0 ml
EUR 134.4

Goat anti-Rat IgG Secondary Antibody (Biotin Conjugate)

GARBI01 150 ug
EUR 123.6

Goat anti-Rat IgG Secondary Antibody (Biotin Conjugate)

GARBI02 1 mg
EUR 313.2

Biotin Conjugated Goat Anti-mouse IgM secondary antibody

BA1004-0.25 0.25ml
EUR 279.6

Biotin Conjugated Goat Anti-mouse IgM secondary antibody

BA1004-0.5 0.5ml
EUR 486

HRP Conjugated Goat Anti-human IgA secondary antibody

BA1066-0.25 0.25ml
EUR 157.2

HRP Conjugated Goat Anti-human IgA secondary antibody

BA1066-0.5 0.5ml
EUR 242.4

HRP Conjugated Goat Anti-human IgM secondary antibody

BA1077-0.25 0.25ml
EUR 157.2

HRP Conjugated Goat Anti-human IgM secondary antibody

BA1077-0.5 0.5ml
EUR 242.4

Goat anti-Mouse IgG-Fc Secondary Antibody (Biotin Conjugate)

GAMGBI01 100 ug
EUR 150

Goat anti-Mouse IgG-Fc Secondary Antibody (Biotin Conjugate)

GAMGBI02 400 ug
EUR 313.2

FITC Conjugated Goat Anti-human IgA secondary antibody

BA1112-0.25 0.25ml
EUR 175.2

FITC Conjugated Goat Anti-human IgA secondary antibody

BA1112-0.5 0.5ml
EUR 279.6

FITC Conjugated Goat Anti-human IgM secondary antibody

BA1116-0.25 0.25ml
EUR 175.2

FITC Conjugated Goat Anti-human IgM secondary antibody

BA1116-0.5 0.5ml
EUR 279.6

Goat Anti-Rabbit Secondary Antibody, Biotin Conjugated

A12001
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

FITC Conjugated Mouse Anti-human IgG (H+L) secondary antibody

BM2003-0.5 0.5mL
EUR 218.4

FITC Conjugated Mouse Anti-human IgG (H+L) secondary antibody

BM2003-1 1mL
EUR 364.8

Biotin Conjugated Goat Anti-human IgM secondary antibody

BA1022-0.25 0.25ml
EUR 279.6

Biotin Conjugated Goat Anti-human IgM secondary antibody

BA1022-0.5 0.5ml
EUR 486

Biotin Conjugated Mouse Anti-human IgG (H+L) secondary antibody

BM2001-0.5 0.5ml
EUR 182.4

Biotin Conjugated Mouse Anti-human IgG (H+L) secondary antibody

BM2001-1 1ml
EUR 412.8

Goat anti-Guinea Pig IgG Secondary Antibody (Biotin Conjugate)

GAGPBI01 150 ug
EUR 150

Goat anti-Guinea Pig IgG Secondary Antibody (Biotin Conjugate)

GAGPBI02 1 mg
EUR 313.2

Cy3 Conjugated Rabbit Anti-Goat IgG (H+L) secondary antibody

BA1034 0.5ml
EUR 218.4

HRP Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1060-0.5 0.5ml
EUR 175.2

HRP Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1060-1 1ml
EUR 279.6

Biotin Conjugated Mouse Anti-rabbit IgG (H+L) secondary antibody

BM2004-0.5 0.5ml
EUR 182.4

Biotin Conjugated Mouse Anti-rabbit IgG (H+L) secondary antibody

BM2004-1 1ml
EUR 412.8

FITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1110-0.5 0.5mL
EUR 157.2

FITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1110-1 1mL
EUR 242.4

TRITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1091-0.5 0.5ml
EUR 224.4

TRITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1091-1 1ml
EUR 400.8

Biotin Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1006-0.5 0.5ml
EUR 115.2

Biotin Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody

BA1006-1 1ml
EUR 157.2

Goat anti-Rabbit IgG-Fc Secondary Antibody (Biotin Conjugate)

GARBBI01 150 ug
EUR 123.6

Goat anti-Rabbit IgG-Fc Secondary Antibody (Biotin Conjugate)

GARBBI02 1 mg
EUR 313.2

Colloidal Gold Conjugate Diluent (10X)

CG-CD10 10 ml
EUR 270

Goat anti-Rat IgG Secondary AssayLite Antibody (APC Conjugate)

GARAPC01 150 ug
EUR 513.6

Goat anti-Rat IgG Secondary AssayLite Antibody (RPE Conjugate)

GARRPE01 150 ug
EUR 513.6

Goat anti-Rat IgG Secondary AssayLite Antibody (FITC Conjugate)

GARFITC01 150 ug
EUR 513.6

Goat anti-Rat IgG Secondary AssayLite Antibody (PerCP Conjugate)

GARPERCP01 150 ug
EUR 565.2

Goat anti-Mouse IgG-Fc Secondary AssayLite Antibody (APC Conjugate)

GAMGAPC01 150 ug
EUR 513.6

These data indicate that sustained expression of the human CFTR transgene in the lungs of mice through a delivery vector can be achieved by repeated temporary immunosuppression.

Nathaniel

Related Posts

Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.

Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.

Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.

A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.

The Protein A-mediated binding of Staphylococcus to antibodies in flow cytometric assays and its reduction using FcR blocking reagent

The Protein A-mediated binding of Staphylococcus to antibodies in flow cytometric assays and its reduction using FcR blocking reagent

No Comment

Leave a Reply

Your email address will not be published.

Recent Posts

Categories

September 2023
M T W T F S S
 123
45678910
11121314151617
18192021222324
252627282930  

Pages