We aim to establish a sensitive and robust assay for estimation of systemic complement activation at the C3 component of complement in mouse and human plasma samples. In order to capture product activation iC3b and C3dg in the relevant specific and physiological manner we utilized construct that consists of sites iC3b / C3dg binds human complement receptor 2 (CR2) is attached to the Fc-portion of IgG mouse.
This construction C3dg and iC3b binding of both mice and humans. We construct CR2-IgG purified from mouse B cell line supernatant myeloma, J558L-CR2-IgG, with protein G affinity chromatography. CR2-IgG construct used to capture fragments of C3 in microtiter wells and anti-mouse or anti-human antibody-C3 is used to detect bound C3 fragments. Initially we tested the specificity of tests using purified fragment C3. Furthermore, using CR2-based test, we measured a signal of up to three-fold higher in activated rat serum compared to non-activated mouse serum, whereas serum activated from C3 knock-out mice did not give a signal.
We tested in vivo produced samples of the experimental mice; complement activation induced by injecting cobra venom factor or heat collected in C57bl6 mouse IgG, followed by the withdrawal of EDTA blood samples at the points of a different time and measurement iC3b / C3dg. We observed a clear time-dependent differences in the signal between samples with expected high and low complement activation. In addition, with the use of an assay to fragment C3 humans,
we observed that patients with systemic lupus erythematosus (SLE) (n = 144) had levels of iC3b / C3dg significantly higher compared with healthy individuals (n = 144) (p <0, 0001). We present two functional immunoassays, which is able to measure the systemic levels of C3 activation product iC3b and C3dg in mice and humans.
To our knowledge, this is the first test for the use of complement activation that are relevant capture physiological constructs such as CR2. This test will be a relevant tool when investigating a mouse model and human diseases involving the complement system.
Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans
Calcium phosphate nanoneedle gene delivery system based immunotherapy of cancer genetics.
Ovarian cancer has become one of the most common gynecologic cancer with a high mortality rate. However, conventional surgery together with combination chemotherapy is difficult to achieve ideal therapeutic effect. Although genetic immunotherapy is applied to an active immune response against the cancer, the lack of efficient in vivo gene delivery technique is still a constraint in clinical applications. To overcome these problems, minicircle DNA vector encoding a human anti-EpCAM / CD3 bispecific antibody (BsAbEPH) has been built.
In addition, various forms of calcium phosphate (Capo) biomaterials prepared. In particular, the capo-nanoneedle-mediated “perforated cell” transfection technology achieve high levels of gene expression in the peritoneal cavity. In intraperitoneal xenograft model with human ovarian cancer cell line SKOV3, Capo-DNA system nanoneedle / minicircle expressed BsAbEPH resulted in significantly retarded the growth of cancer and mouse extension of life span with limited toxicity.
And the system can be made as an off-the-shelf and products that are easy to use. Therefore, Capo-nanoneedle technology-based non-viral gene delivery will have great potential in clinical application.Correspondingly, human CFTR transgene expression was increased significantly by administration of cyclophosphamide compared with the group without treatment.
10 nm Colloidal Gold Conjugated Protein G
GA1056
BosterBio
1ml
EUR 206.4
Goat Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10119
Alpha Diagnostics
500 ul
EUR 270
Goat anti-rabbit IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-20119
Alpha Diagnostics
500 ul
EUR 270
Rabbit Anti-Goat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-30319
Alpha Diagnostics
500 ul
EUR 270
Goat Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-50119
Alpha Diagnostics
500 ul
EUR 270
Goat Anti-mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-40320
Alpha Diagnostics
500 ul
EUR 270
Goat anti Mouse IgG (10 nm Gold Colloid)
43R-IG084GD
Fitzgerald
1 ml
EUR 846
Description: Goat anti Mouse IgG secondary antibody (10 nm Gold Colloid)
Goat Anti-Human IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10319
Alpha Diagnostics
500 ul
EUR 270
Goat anti-rabbit IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-20319
Alpha Diagnostics
500 ul
EUR 270
Rabbit anti-Goat IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-30219
Alpha Diagnostics
500 ul
EUR 270
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RMG-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RMG-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RMG-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RMG-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RMG-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RMG-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RMG-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RMG-10-980
Creative Diagnostics
1 mL
EUR 1226.4
Rabbit anti-Mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10
CG-40319
Alpha Diagnostics
500 ul
EUR 270
Rabbit Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10119-R
Alpha Diagnostics
500 ul
EUR 270
Rabbit Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-50119-R
Alpha Diagnostics
500 ul
EUR 270
Goat Anti-Mouse IgG (H+L)-Gold (~10 nm) Conjugate
40319-G10
Alpha Diagnostics
0.20 ml
EUR 242.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RRG-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RRG-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RRG-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RRG-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RRG-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RRG-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RRG-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RRG-10-980
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RHG-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RHG-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RHG-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RHG-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RHG-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RHG-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RHG-10-980
Creative Diagnostics
1 mL
EUR 1226.4
Streptavidin colloidal gold conjugated (40 nm, OD10)
CG-ST40
Alpha Diagnostics
500 ul
EUR 270
5 nm Colloidal Gold Conjugated Protein A
GA1053
BosterBio
1ml
EUR 206.4
5 nm Colloidal Gold Conjugated Protein G
GA1055
BosterBio
1ml
EUR 206.4
Rabbit Anti-Goat IgG (H+L)-Gold (~10 nm) Conjugate
30219-G10
Alpha Diagnostics
0.20 ml
EUR 242.4
Rabbit Anti-Goat IgG (H+L)-Gold (10 nm) conjugate
30222G
Alpha Diagnostics
0.5 ml
EUR 242.4
Goat anti Mouse IgG (20 nm Gold Colloid)
43R-IG087GD
Fitzgerald
1 ml
EUR 846
Description: Goat anti Mouse IgG secondary antibody (20 nm Gold Colloid)
Goat Anti-Human IgM (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10219
Alpha Diagnostics
500 ul
EUR 300
Protein A colloidal gold conjugated (40 nm, OD10)
CG-PRTA40
Alpha Diagnostics
500 ul
EUR 270
Protein G colloidal gold conjugated (40 nm, OD10)
CG-PRTG40
Alpha Diagnostics
500 ul
EUR 270
Goat Anti-Mouse IgG Secondary Antibody AF790 Conjugated
L30310
SAB
0.5 ml
EUR 151.2
Goat Anti-Mouse IgG Secondary Antibody Cy3 Conjugated
L30311
SAB
1.0 ml
EUR 134.4
Goat Anti-Mouse IgG Secondary Antibody FITC Conjugated
L30313
SAB
1.0 ml
EUR 116.4
Goat Anti-Mouse IgG Secondary Antibody APC Conjugated
L30316
SAB
0.5 ml
EUR 229.2
Goat Anti-Mouse IgG Secondary Antibody PE Conjugated
L30317
SAB
1.0 ml
EUR 229.2
Goat Anti-Mouse IgG Secondary Antibody PerCP Conjugated
L30318
SAB
0.5 ml
EUR 229.2
Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated
L3032
SAB
1.0 ml
EUR 134.4
Goat Anti-Mouse IgG Secondary Antibody AP Conjugated
L3033
SAB
1.0 ml
EUR 142.8
Goat Anti-Mouse IgG Secondary Antibody Biotin Conjugated
L3034
SAB
1.0 ml
EUR 124.8
Goat Anti-Mouse IgG Secondary Antibody AMCA Conjugated
L3035
SAB
1.0 ml
EUR 116.4
Goat Anti-Mouse IgG Secondary Antibody AF488 Conjugated
L3036
SAB
1.0 ml
EUR 134.4
Goat Anti-Mouse IgG Secondary Antibody AF594 Conjugated
L3037
SAB
1.0 ml
EUR 134.4
Goat Anti-Mouse IgG Secondary Antibody AF647 Conjugated
L3038
SAB
1.0 ml
EUR 134.4
Goat Anti-Mouse IgG Secondary Antibody AF680 Conjugated
L3039
SAB
0.5 ml
EUR 151.2
Goat anti Rabbit IgG (15 nm Gold Colloid)
43R-IG085GD
Fitzgerald
1 ml
EUR 846
Description: Goat anti Rabbit IgG secondary antibody (15 nm Gold Colloid)
Goat anti Rabbit IgG (20 nm Gold Colloid)
43R-IG086GD
Fitzgerald
1 ml
EUR 878.4
Description: Goat anti Rabbit IgG secondary antibody (20 nm Gold Colloid)
Goat anti Rabbit IgG (5 nm Gold Colloid)
43R-IG100GD
Fitzgerald
1 ml
EUR 846
Description: Goat anti Rabbit IgG secondary antibody (5 nm Gold Colloid)
Mouse monoclonal anti-Human IgG1 (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10121
Alpha Diagnostics
500 ul
EUR 300
Protein A/G colloidal gold conjugated (40 nm, OD10)
CG-PRTAG40
Alpha Diagnostics
500 ul
EUR 270
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RHA-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RHA-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RHA-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RHA-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RHA-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RHA-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RHM-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RHM-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RHM-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RHM-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RHM-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RHM-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RHM-10-980
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RCG-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RCG-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RCG-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RCG-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RCG-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RCG-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RCG-10-980
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RCN-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RCN-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RCN-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RCN-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RCN-10-808
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RCN-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RCN-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RCS-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RCS-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RCS-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RCS-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RCS-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RCS-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RCS-10-980
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RGS-10-1064
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RGS-10-700
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RGS-10-750
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RGS-10-780
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RGS-10-850
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RGS-10-900
Creative Diagnostics
1 mL
EUR 1226.4
DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RGS-10-980
Creative Diagnostics
1 mL
EUR 1226.4
Goat Anti-Mouse IgG Secondary Antibody Rhodamine (TRITC) Conjugated
L30314
SAB
1.0 ml
EUR 116.4
Goat Anti-Mouse IgG Secondary Antibody Rhodamine (RRX) Conjugated
L30315
SAB
1.0 ml
EUR 116.4
Gold Colloid (10 nm)
62R-GC010
Fitzgerald
100 ml
EUR 560.4
Description: 10 nm Colloidal Gold particle suspension
Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated
L30111
SAB
1.0 ml
EUR 134.4
Goat Anti-Rabbit IgG Secondary Antibody FITC Conjugated
L30113
SAB
1.0 ml
EUR 116.4
Goat Anti-Rabbit IgG Secondary Antibody APC Conjugated
L30116
SAB
0.5 ml
EUR 229.2
Goat Anti-Rabbit IgG Secondary Antibody PE Conjugated
L30117
SAB
1.0 ml
EUR 229.2
Goat Anti-Rabbit IgG Secondary Antibody PerCP Conjugated
L30118
SAB
0.5 ml
EUR 229.2
Goat Anti-Rabbit IgG Secondary Antibody HRP Conjugated
L3012
SAB
1.0 ml
EUR 134.4
Goat Anti-Rabbit IgG Secondary Antibody Biotin Conjugated
L3014
SAB
1.0 ml
EUR 124.8
Goat Anti-Rabbit IgG Secondary Antibody AF488 Conjugated
L3016
SAB
1.0 ml
EUR 134.4
Goat Anti-Rabbit IgG Secondary Antibody AF594 Conjugated
L3017
SAB
1.0 ml
EUR 134.4
Goat Anti-Rabbit IgG Secondary Antibody AF647 Conjugated
L3018
SAB
1.0 ml
EUR 134.4
Goat Anti-Human IgG Secondary Antibody Cy3 Conjugated
L30211
SAB
1.0 ml
EUR 134.4
Goat Anti-Human IgG Secondary Antibody FITC Conjugated
L30213
SAB
1.0 ml
EUR 116.4
Goat Anti-Human IgG Secondary Antibody APC Conjugated
L30216
SAB
0.5 ml
EUR 229.2
Goat Anti-Human IgG Secondary Antibody PE Conjugated
L30217
SAB
1.0 ml
EUR 229.2
Goat Anti-Human IgG Secondary Antibody PerCP Conjugated
L30218
SAB
0.5 ml
EUR 229.2
These data indicate that sustained expression of the human CFTR transgene in the lungs of mice through a delivery vector can be achieved by repeated temporary immunosuppression.
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