We aim to establish a sensitive and robust assay for estimation of systemic complement activation at the C3 component of complement in mouse and human plasma samples. In order to capture product activation iC3b and C3dg in the relevant specific and physiological manner we utilized construct that consists of sites iC3b / C3dg binds human complement receptor 2 (CR2) is attached to the Fc-portion of IgG mouse.

This construction C3dg and iC3b binding of both mice and humans. We construct CR2-IgG purified from mouse B cell line supernatant myeloma, J558L-CR2-IgG, with protein G affinity chromatography. CR2-IgG construct used to capture fragments of C3 in microtiter wells and anti-mouse or anti-human antibody-C3 is used to detect bound C3 fragments. Initially we tested the specificity of tests using purified fragment C3. Furthermore, using CR2-based test, we measured a signal of up to three-fold higher in activated rat serum compared to non-activated mouse serum, whereas serum activated from C3 knock-out mice did not give a signal.

We tested in vivo produced samples of the experimental mice; complement activation induced by injecting cobra venom factor or heat collected in C57bl6 mouse IgG, followed by the withdrawal of EDTA blood samples at the points of a different time and measurement iC3b / C3dg. We observed a clear time-dependent differences in the signal between samples with expected high and low complement activation. In addition, with the use of an assay to fragment C3 humans,

we observed that patients with systemic lupus erythematosus (SLE) (n = 144) had levels of iC3b / C3dg significantly higher compared with healthy individuals (n = 144) (p <0, 0001). We present two functional immunoassays, which is able to measure the systemic levels of C3 activation product iC3b and C3dg in mice and humans.

To our knowledge, this is the first test for the use of complement activation that are relevant capture physiological constructs such as CR2. This test will be a relevant tool when investigating a mouse model and human diseases involving the complement system.

Calcium phosphate nanoneedle gene delivery system based immunotherapy of cancer genetics.

Ovarian cancer has become one of the most common gynecologic cancer with a high mortality rate. However, conventional surgery together with combination chemotherapy is difficult to achieve ideal therapeutic effect. Although genetic immunotherapy is applied to an active immune response against the cancer, the lack of efficient in vivo gene delivery technique is still a constraint in clinical applications. To overcome these problems, minicircle DNA vector encoding a human anti-EpCAM / CD3 bispecific antibody (BsAbEPH) has been built.

In addition, various forms of calcium phosphate (Capo) biomaterials prepared. In particular, the capo-nanoneedle-mediated “perforated cell” transfection technology achieve high levels of gene expression in the peritoneal cavity. In intraperitoneal xenograft model with human ovarian cancer cell line SKOV3, Capo-DNA system nanoneedle / minicircle expressed BsAbEPH resulted in significantly retarded the growth of cancer and mouse extension of life span with limited toxicity.

And the system can be made as an off-the-shelf and products that are easy to use. Therefore, Capo-nanoneedle technology-based non-viral gene delivery will have great potential in clinical application.Correspondingly, human CFTR transgene expression was increased significantly by administration of cyclophosphamide compared with the group without treatment.

10 nm Colloidal Gold Conjugated Protein G
GA1056	BosterBio	1ml	EUR 206.4

Goat Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10119	Alpha Diagnostics	500 ul	EUR 270

Goat anti-rabbit IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-20119	Alpha Diagnostics	500 ul	EUR 270

Rabbit Anti-Goat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-30319	Alpha Diagnostics	500 ul	EUR 270

Goat Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-50119	Alpha Diagnostics	500 ul	EUR 270

Goat Anti-mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-40320	Alpha Diagnostics	500 ul	EUR 270

Goat anti Mouse IgG (10 nm Gold Colloid)
43R-IG084GD	Fitzgerald	1 ml	EUR 846
Description: Goat anti Mouse IgG secondary antibody (10 nm Gold Colloid)

Goat Anti-Human IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10319	Alpha Diagnostics	500 ul	EUR 270

Goat anti-rabbit IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-20319	Alpha Diagnostics	500 ul	EUR 270

Rabbit anti-Goat IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-30219	Alpha Diagnostics	500 ul	EUR 270

Colloidal Gold (unconjugated) 10 nm, 1 OD
CG-10NM	Alpha Diagnostics	100 ml	EUR 498

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RMG-10-1064	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RMG-10-700	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RMG-10-750	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RMG-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RMG-10-808	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RMG-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RMG-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Mouse IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RMG-10-980	Creative Diagnostics	1 mL	EUR 1226.4

Rabbit anti-Mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10
CG-40319	Alpha Diagnostics	500 ul	EUR 270

Rabbit Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10119-R	Alpha Diagnostics	500 ul	EUR 270

Rabbit Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-50119-R	Alpha Diagnostics	500 ul	EUR 270

Goat Anti-Mouse IgG (H+L)-Gold (~10 nm) Conjugate
40319-G10	Alpha Diagnostics	0.20 ml	EUR 242.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RRG-10-1064	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RRG-10-700	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RRG-10-750	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RRG-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RRG-10-808	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RRG-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RRG-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Rabbit IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RRG-10-980	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RHG-10-1064	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RHG-10-750	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RHG-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RHG-10-808	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RHG-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RHG-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgG conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RHG-10-980	Creative Diagnostics	1 mL	EUR 1226.4

Streptavidin colloidal gold conjugated (40 nm, OD10)
CG-ST40	Alpha Diagnostics	500 ul	EUR 270

5 nm Colloidal Gold Conjugated Protein A
GA1053	BosterBio	1ml	EUR 206.4

5 nm Colloidal Gold Conjugated Protein G
GA1055	BosterBio	1ml	EUR 206.4

Rabbit Anti-Goat IgG (H+L)-Gold (~10 nm) Conjugate
30219-G10	Alpha Diagnostics	0.20 ml	EUR 242.4

Rabbit Anti-Goat IgG (H+L)-Gold (10 nm) conjugate
30222G	Alpha Diagnostics	0.5 ml	EUR 242.4

Goat anti Mouse IgG (20 nm Gold Colloid)
43R-IG087GD	Fitzgerald	1 ml	EUR 846
Description: Goat anti Mouse IgG secondary antibody (20 nm Gold Colloid)

Goat Anti-Human IgM (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10219	Alpha Diagnostics	500 ul	EUR 300

Protein A colloidal gold conjugated (40 nm, OD10)
CG-PRTA40	Alpha Diagnostics	500 ul	EUR 270

Protein G colloidal gold conjugated (40 nm, OD10)
CG-PRTG40	Alpha Diagnostics	500 ul	EUR 270

Goat Anti-Mouse IgG Secondary Antibody AF790 Conjugated
L30310	SAB	0.5 ml	EUR 151.2

Goat Anti-Mouse IgG Secondary Antibody Cy3 Conjugated
L30311	SAB	1.0 ml	EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody FITC Conjugated
L30313	SAB	1.0 ml	EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody APC Conjugated
L30316	SAB	0.5 ml	EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody PE Conjugated
L30317	SAB	1.0 ml	EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody PerCP Conjugated
L30318	SAB	0.5 ml	EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated
L3032	SAB	1.0 ml	EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AP Conjugated
L3033	SAB	1.0 ml	EUR 142.8

Goat Anti-Mouse IgG Secondary Antibody Biotin Conjugated
L3034	SAB	1.0 ml	EUR 124.8

Goat Anti-Mouse IgG Secondary Antibody AMCA Conjugated
L3035	SAB	1.0 ml	EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody AF488 Conjugated
L3036	SAB	1.0 ml	EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF594 Conjugated
L3037	SAB	1.0 ml	EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF647 Conjugated
L3038	SAB	1.0 ml	EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF680 Conjugated
L3039	SAB	0.5 ml	EUR 151.2

Goat anti Rabbit IgG (15 nm Gold Colloid)
43R-IG085GD	Fitzgerald	1 ml	EUR 846
Description: Goat anti Rabbit IgG secondary antibody (15 nm Gold Colloid)

Goat anti Rabbit IgG (20 nm Gold Colloid)
43R-IG086GD	Fitzgerald	1 ml	EUR 878.4
Description: Goat anti Rabbit IgG secondary antibody (20 nm Gold Colloid)

Goat anti Rabbit IgG (5 nm Gold Colloid)
43R-IG100GD	Fitzgerald	1 ml	EUR 846
Description: Goat anti Rabbit IgG secondary antibody (5 nm Gold Colloid)

Mouse monoclonal anti-Human IgG1 (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10121	Alpha Diagnostics	500 ul	EUR 300

Protein A/G colloidal gold conjugated (40 nm, OD10)
CG-PRTAG40	Alpha Diagnostics	500 ul	EUR 270

DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RHA-10-1064	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RHA-10-700	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RHA-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RHA-10-808	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RHA-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgA conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RHA-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RHM-10-700	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RHM-10-750	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RHM-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RHM-10-808	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RHM-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RHM-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Anti-Human IgM conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RHM-10-980	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Silica-Coated Gold Nanoparticles, 10 nm
CG-10	Creative Diagnostics	10mL	EUR 862.8

DiagNano Fluorophore Labeled Gold Nanoparticles, 10 nm
GFL-10	Creative Diagnostics	1 mL	EUR 1263.6

DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RCG-10-1064	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RCG-10-700	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RCG-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RCG-10-808	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RCG-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RCG-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Galactose conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RCG-10-980	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RCN-10-1064	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RCN-10-700	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RCN-10-750	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RCN-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 808 nm
RCN-10-808	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RCN-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano NeutrAvidin conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RCN-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RCS-10-1064	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RCS-10-700	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RCS-10-750	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RCS-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RCS-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RCS-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano Streptavidin conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RCS-10-980	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 1064 nm
RGS-10-1064	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 700 nm
RGS-10-700	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 750 nm
RGS-10-750	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 780 nm
RGS-10-780	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 850 nm
RGS-10-850	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 900 nm
RGS-10-900	Creative Diagnostics	1 mL	EUR 1226.4

DiagNano GSH conjugated Gold Nanorods, diameter 10 nm, absorption max 980 nm
RGS-10-980	Creative Diagnostics	1 mL	EUR 1226.4

Colloidal Gold (unconjugated) 20 nm, 1 OD
CG-20NM	Alpha Diagnostics	100 ml	EUR 498

Colloidal Gold (unconjugated) 40 nm, 1 OD
CG-40NM	Alpha Diagnostics	100 ml	EUR 424.8

Goat Anti-Mouse IgG Secondary Antibody Rhodamine (TRITC) Conjugated
L30314	SAB	1.0 ml	EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody Rhodamine (RRX) Conjugated
L30315	SAB	1.0 ml	EUR 116.4

Gold Colloid (10 nm)
62R-GC010	Fitzgerald	100 ml	EUR 560.4
Description: 10 nm Colloidal Gold particle suspension

Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated
L30111	SAB	1.0 ml	EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody FITC Conjugated
L30113	SAB	1.0 ml	EUR 116.4

Goat Anti-Rabbit IgG Secondary Antibody APC Conjugated
L30116	SAB	0.5 ml	EUR 229.2

Goat Anti-Rabbit IgG Secondary Antibody PE Conjugated
L30117	SAB	1.0 ml	EUR 229.2

Goat Anti-Rabbit IgG Secondary Antibody PerCP Conjugated
L30118	SAB	0.5 ml	EUR 229.2

Goat Anti-Rabbit IgG Secondary Antibody HRP Conjugated
L3012	SAB	1.0 ml	EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody Biotin Conjugated
L3014	SAB	1.0 ml	EUR 124.8

Goat Anti-Rabbit IgG Secondary Antibody AF488 Conjugated
L3016	SAB	1.0 ml	EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody AF594 Conjugated
L3017	SAB	1.0 ml	EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody AF647 Conjugated
L3018	SAB	1.0 ml	EUR 134.4

Goat Anti-Human IgG Secondary Antibody Cy3 Conjugated
L30211	SAB	1.0 ml	EUR 134.4

Goat Anti-Human IgG Secondary Antibody FITC Conjugated
L30213	SAB	1.0 ml	EUR 116.4

Goat Anti-Human IgG Secondary Antibody APC Conjugated
L30216	SAB	0.5 ml	EUR 229.2

Goat Anti-Human IgG Secondary Antibody PE Conjugated
L30217	SAB	1.0 ml	EUR 229.2

Goat Anti-Human IgG Secondary Antibody PerCP Conjugated
L30218	SAB	0.5 ml	EUR 229.2

These data indicate that sustained expression of the human CFTR transgene in the lungs of mice through a delivery vector can be achieved by repeated temporary immunosuppression.