We aim to establish a sensitive and robust assay for estimation of systemic complement activation at the C3 component of complement in mouse and human plasma samples. In order to capture product activation iC3b and C3dg in the relevant specific and physiological manner we utilized construct that consists of sites iC3b / C3dg binds human complement receptor 2 (CR2) is attached to the Fc-portion of IgG mouse.

This construction C3dg and iC3b binding of both mice and humans. We construct CR2-IgG purified from mouse B cell line supernatant myeloma, J558L-CR2-IgG, with protein G affinity chromatography. CR2-IgG construct used to capture fragments of C3 in microtiter wells and anti-mouse or anti-human antibody-C3 is used to detect bound C3 fragments. Initially we tested the specificity of tests using purified fragment C3. Furthermore, using CR2-based test, we measured a signal of up to three-fold higher in activated rat serum compared to non-activated mouse serum, whereas serum activated from C3 knock-out mice did not give a signal.

We tested in vivo produced samples of the experimental mice; complement activation induced by injecting cobra venom factor or heat collected in C57bl6 mouse IgG, followed by the withdrawal of EDTA blood samples at the points of a different time and measurement iC3b / C3dg. We observed a clear time-dependent differences in the signal between samples with expected high and low complement activation. In addition, with the use of an assay to fragment C3 humans,

we observed that patients with systemic lupus erythematosus (SLE) (n = 144) had levels of iC3b / C3dg significantly higher compared with healthy individuals (n = 144) (p <0, 0001). We present two functional immunoassays, which is able to measure the systemic levels of C3 activation product iC3b and C3dg in mice and humans.

To our knowledge, this is the first test for the use of complement activation that are relevant capture physiological constructs such as CR2. This test will be a relevant tool when investigating a mouse model and human diseases involving the complement system.

Calcium phosphate nanoneedle gene delivery system based immunotherapy of cancer genetics.

Ovarian cancer has become one of the most common gynecologic cancer with a high mortality rate. However, conventional surgery together with combination chemotherapy is difficult to achieve ideal therapeutic effect. Although genetic immunotherapy is applied to an active immune response against the cancer, the lack of efficient in vivo gene delivery technique is still a constraint in clinical applications. To overcome these problems, minicircle DNA vector encoding a human anti-EpCAM / CD3 bispecific antibody (BsAbEPH) has been built.

In addition, various forms of calcium phosphate (Capo) biomaterials prepared. In particular, the capo-nanoneedle-mediated “perforated cell” transfection technology achieve high levels of gene expression in the peritoneal cavity. In intraperitoneal xenograft model with human ovarian cancer cell line SKOV3, Capo-DNA system nanoneedle / minicircle expressed BsAbEPH resulted in significantly retarded the growth of cancer and mouse extension of life span with limited toxicity.

And the system can be made as an off-the-shelf and products that are easy to use. Therefore, Capo-nanoneedle technology-based non-viral gene delivery will have great potential in clinical application.Correspondingly, human CFTR transgene expression was increased significantly by administration of cyclophosphamide compared with the group without treatment.

Goat Anti-mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-40320	Alpha Diagnostics	500 ul	EUR 270

Goat Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10119	Alpha Diagnostics	500 ul	EUR 270

Goat anti-rabbit IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-20119	Alpha Diagnostics	500 ul	EUR 270

Goat Anti-Human IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10319	Alpha Diagnostics	500 ul	EUR 270

Goat anti-rabbit IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-20319	Alpha Diagnostics	500 ul	EUR 270

Rabbit Anti-Goat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-30319	Alpha Diagnostics	500 ul	EUR 270

Rabbit anti-Goat IgG (H+L) colloidal gold conjugated (40 nm, OD10)
CG-30219	Alpha Diagnostics	500 ul	EUR 270

10 nm Colloidal Gold Conjugated Protein A
GA1054	BosterBio	1ml	EUR 206.4

10 nm Colloidal Gold Conjugated Protein G
GA1056	BosterBio	1ml	EUR 206.4

Goat Anti-Human IgM (H+L) colloidal gold conjugated (40 nm, OD10)
CG-10219	Alpha Diagnostics	500 ul	EUR 300

Rabbit Anti-Rat IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-50119-R	Alpha Diagnostics	500 ul	EUR 270

Rabbit anti-Mouse IgG (H+L) colloidal gold conjugated (40 nm, OD10
CG-40319	Alpha Diagnostics	500 ul	EUR 270

Rabbit Anti-Human IgG (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10119-R	Alpha Diagnostics	500 ul	EUR 270

Mouse monoclonal anti-Human IgG1 (Fc) colloidal gold conjugated (40 nm, OD10)
CG-10121	Alpha Diagnostics	500 ul	EUR 300

Goat Anti-Mouse IgG Secondary Antibody PE Conjugated
L30317	SAB	1.0 ml	EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody AP Conjugated
L3033	SAB	1.0 ml	EUR 142.8

Goat Anti-Mouse IgG Secondary Antibody Cy3 Conjugated
L30311	SAB	1.0 ml	EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody APC Conjugated
L30316	SAB	0.5 ml	EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated
L3032	SAB	1.0 ml	EUR 134.4

5 nm Colloidal Gold Conjugated Protein A
GA1053	BosterBio	1ml	EUR 206.4

5 nm Colloidal Gold Conjugated Protein G
GA1055	BosterBio	1ml	EUR 206.4

Goat Anti-Mouse IgG Secondary Antibody FITC Conjugated
L30313	SAB	1.0 ml	EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody AMCA Conjugated
L3035	SAB	1.0 ml	EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody AF790 Conjugated
L30310	SAB	0.5 ml	EUR 151.2

Goat Anti-Mouse IgG Secondary Antibody PerCP Conjugated
L30318	SAB	0.5 ml	EUR 229.2

Goat Anti-Mouse IgG Secondary Antibody AF488 Conjugated
L3036	SAB	1.0 ml	EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF594 Conjugated
L3037	SAB	1.0 ml	EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF647 Conjugated
L3038	SAB	1.0 ml	EUR 134.4

Goat Anti-Mouse IgG Secondary Antibody AF680 Conjugated
L3039	SAB	0.5 ml	EUR 151.2

Goat Anti-Mouse IgG Secondary Antibody Biotin Conjugated
L3034	SAB	1.0 ml	EUR 124.8

Goat Anti-Human IgG Secondary Antibody PE Conjugated
L30217	SAB	1.0 ml	EUR 229.2

Goat Anti-Human IgG Secondary Antibody AP Conjugated
L3023	SAB	1.0 ml	EUR 142.8

Streptavidin colloidal gold conjugated (40 nm, OD10)
CG-ST40	Alpha Diagnostics	500 ul	EUR 270

Goat Anti-Rabbit IgG Secondary Antibody PE Conjugated
L30117	SAB	1.0 ml	EUR 229.2

Goat Anti-Human IgG Secondary Antibody Cy3 Conjugated
L30211	SAB	1.0 ml	EUR 134.4

Goat Anti-Human IgG Secondary Antibody APC Conjugated
L30216	SAB	0.5 ml	EUR 229.2

Goat Anti-Human IgG Secondary Antibody HRP Conjugated
L3022	SAB	1.0 ml	EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody AP Conjugated
L3073	SAB	1.0 ml	EUR 142.8

Cy3 Conjugated Goat Anti-Mouse IgG (H+L) secondary antibody
BA1031	BosterBio	0.5ml	EUR 218.4

HRP Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1050-0.5	BosterBio	0.5ml	EUR 175.2

HRP Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1050-1	BosterBio	1ml	EUR 279.6

Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated
L30111	SAB	1.0 ml	EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody APC Conjugated
L30116	SAB	0.5 ml	EUR 229.2

Goat Anti-Rabbit IgG Secondary Antibody HRP Conjugated
L3012	SAB	1.0 ml	EUR 134.4

Goat Anti-Human IgG Secondary Antibody FITC Conjugated
L30213	SAB	1.0 ml	EUR 116.4

Goat Anti-Human IgG Secondary Antibody AMCA Conjugated
L3025	SAB	1.0 ml	EUR 116.4

FITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1101-0.5	BosterBio	0.5mL	EUR 157.2

FITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1101-1	BosterBio	1mL	EUR 242.4

Protein A colloidal gold conjugated (40 nm, OD10)
CG-PRTA40	Alpha Diagnostics	500 ul	EUR 270

Protein G colloidal gold conjugated (40 nm, OD10)
CG-PRTG40	Alpha Diagnostics	500 ul	EUR 270

TRITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1089-0.5	BosterBio	0.5ml	EUR 224.4

TRITC Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1089-1	BosterBio	1ml	EUR 400.8

Goat Anti-Rabbit IgG Secondary Antibody FITC Conjugated
L30113	SAB	1.0 ml	EUR 116.4

Goat Anti-Human IgG Secondary Antibody PerCP Conjugated
L30218	SAB	0.5 ml	EUR 229.2

Goat Anti-Human IgG Secondary Antibody AF488 Conjugated
L3026	SAB	1.0 ml	EUR 134.4

Goat Anti-Human IgG Secondary Antibody AF594 Conjugated
L3027	SAB	1.0 ml	EUR 134.4

Goat Anti-Human IgG Secondary Antibody AF647 Conjugated
L3028	SAB	1.0 ml	EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody AMCA Conjugated
L3065	SAB	1.0 ml	EUR 116.4

Biotin Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1001-0.5	BosterBio	0.5ml	EUR 115.2

Biotin Conjugated Goat Anti-mouse IgG (H+L) secondary antibody
BA1001-1	BosterBio	1ml	EUR 157.2

Goat Anti-Rabbit IgG Secondary Antibody PerCP Conjugated
L30118	SAB	0.5 ml	EUR 229.2

Goat Anti-Rabbit IgG Secondary Antibody AF488 Conjugated
L3016	SAB	1.0 ml	EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody AF594 Conjugated
L3017	SAB	1.0 ml	EUR 134.4

Goat Anti-Rabbit IgG Secondary Antibody AF647 Conjugated
L3018	SAB	1.0 ml	EUR 134.4

Goat Anti-Human IgG Secondary Antibody Biotin Conjugated
L3024	SAB	1.0 ml	EUR 124.8

Goat Anti-Rabbit IgG Secondary Antibody AF680 Conjugated
L3049	SAB	0.5 ml	EUR 151.2

Goat Anti-Rabbit IgG Secondary Antibody AF790 Conjugated
L30510	SAB	0.5 ml	EUR 151.2

Goat Anti-Rabbit IgG Secondary Antibody Biotin Conjugated
L3014	SAB	1.0 ml	EUR 124.8

Goat Anti-Mouse IgG Secondary Antibody Rhodamine (RRX) Conjugated
L30315	SAB	1.0 ml	EUR 116.4

Goat Anti-Mouse IgG Secondary Antibody Rhodamine (TRITC) Conjugated
L30314	SAB	1.0 ml	EUR 116.4

Protein A/G colloidal gold conjugated (40 nm, OD10)
CG-PRTAG40	Alpha Diagnostics	500 ul	EUR 270

Cy3 Conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody
BA1032	BosterBio	0.5ml	EUR 218.4

HRP Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1054-0.5	BosterBio	0.5ml	EUR 175.2

HRP Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1054-1	BosterBio	1ml	EUR 279.6

FITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1105-0.5	BosterBio	0.5mL	EUR 157.2

FITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1105-1	BosterBio	1mL	EUR 242.4

TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1090-0.5	BosterBio	0.5ml	EUR 224.4

TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1090-1	BosterBio	1ml	EUR 400.8

Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1003-0.5	BosterBio	0.5ml	EUR 115.2

Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody
BA1003-1	BosterBio	1ml	EUR 157.2

Goat Anti-Human IgG Secondary Antibody Rhodamine (RRX) Conjugated
L30215	SAB	1.0 ml	EUR 116.4

Goat Anti-Rabbit IgG Secondary Antibody Rhodamine (RRX) Conjugated
L30115	SAB	1.0 ml	EUR 116.4

Goat Anti-Human IgG Secondary Antibody Rhodamine (TRITC) Conjugated
L30214	SAB	1.0 ml	EUR 116.4

Goat Anti-Rabbit IgG Secondary Antibody Rhodamine (TRITC) Conjugated
L30114	SAB	1.0 ml	EUR 116.4

HRP Conjugated Goat Anti-mouse IgM secondary antibody
BA1075-0.25	BosterBio	0.25ml	EUR 175.2

HRP Conjugated Goat Anti-mouse IgM secondary antibody
BA1075-0.5	BosterBio	0.5ml	EUR 279.6

Rabbit anti-Goat IgG Secondary Antibody HRP conjugated
L3042	SAB	1.0 ml	EUR 134.4

Goat anti-Rat IgG Secondary Antibody (Biotin Conjugate)
GARBI01	AssayPro	150 ug	EUR 123.6

Goat anti-Rat IgG Secondary Antibody (Biotin Conjugate)
GARBI02	AssayPro	1 mg	EUR 313.2

Biotin Conjugated Goat Anti-mouse IgM secondary antibody
BA1004-0.25	BosterBio	0.25ml	EUR 279.6

Biotin Conjugated Goat Anti-mouse IgM secondary antibody
BA1004-0.5	BosterBio	0.5ml	EUR 486

HRP Conjugated Goat Anti-human IgA secondary antibody
BA1066-0.25	BosterBio	0.25ml	EUR 157.2

HRP Conjugated Goat Anti-human IgA secondary antibody
BA1066-0.5	BosterBio	0.5ml	EUR 242.4

HRP Conjugated Goat Anti-human IgM secondary antibody
BA1077-0.25	BosterBio	0.25ml	EUR 157.2

HRP Conjugated Goat Anti-human IgM secondary antibody
BA1077-0.5	BosterBio	0.5ml	EUR 242.4

Goat anti-Mouse IgG-Fc Secondary Antibody (Biotin Conjugate)
GAMGBI01	AssayPro	100 ug	EUR 150

Goat anti-Mouse IgG-Fc Secondary Antibody (Biotin Conjugate)
GAMGBI02	AssayPro	400 ug	EUR 313.2

FITC Conjugated Goat Anti-human IgA secondary antibody
BA1112-0.25	BosterBio	0.25ml	EUR 175.2

FITC Conjugated Goat Anti-human IgA secondary antibody
BA1112-0.5	BosterBio	0.5ml	EUR 279.6

FITC Conjugated Goat Anti-human IgM secondary antibody
BA1116-0.25	BosterBio	0.25ml	EUR 175.2

FITC Conjugated Goat Anti-human IgM secondary antibody
BA1116-0.5	BosterBio	0.5ml	EUR 279.6

Goat Anti-Rabbit Secondary Antibody, Biotin Conjugated
A12001	EpiGentek	EUR 232.26 EUR 117.70	350 µg 350 ug

FITC Conjugated Mouse Anti-human IgG (H+L) secondary antibody
BM2003-0.5	BosterBio	0.5mL	EUR 218.4

FITC Conjugated Mouse Anti-human IgG (H+L) secondary antibody
BM2003-1	BosterBio	1mL	EUR 364.8

Biotin Conjugated Goat Anti-human IgM secondary antibody
BA1022-0.25	BosterBio	0.25ml	EUR 279.6

Biotin Conjugated Goat Anti-human IgM secondary antibody
BA1022-0.5	BosterBio	0.5ml	EUR 486

Biotin Conjugated Mouse Anti-human IgG (H+L) secondary antibody
BM2001-0.5	BosterBio	0.5ml	EUR 182.4

Biotin Conjugated Mouse Anti-human IgG (H+L) secondary antibody
BM2001-1	BosterBio	1ml	EUR 412.8

Goat anti-Guinea Pig IgG Secondary Antibody (Biotin Conjugate)
GAGPBI01	AssayPro	150 ug	EUR 150

Goat anti-Guinea Pig IgG Secondary Antibody (Biotin Conjugate)
GAGPBI02	AssayPro	1 mg	EUR 313.2

Cy3 Conjugated Rabbit Anti-Goat IgG (H+L) secondary antibody
BA1034	BosterBio	0.5ml	EUR 218.4

HRP Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1060-0.5	BosterBio	0.5ml	EUR 175.2

HRP Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1060-1	BosterBio	1ml	EUR 279.6

Biotin Conjugated Mouse Anti-rabbit IgG (H+L) secondary antibody
BM2004-0.5	BosterBio	0.5ml	EUR 182.4

Biotin Conjugated Mouse Anti-rabbit IgG (H+L) secondary antibody
BM2004-1	BosterBio	1ml	EUR 412.8

FITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1110-0.5	BosterBio	0.5mL	EUR 157.2

FITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1110-1	BosterBio	1mL	EUR 242.4

TRITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1091-0.5	BosterBio	0.5ml	EUR 224.4

TRITC Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1091-1	BosterBio	1ml	EUR 400.8

Biotin Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1006-0.5	BosterBio	0.5ml	EUR 115.2

Biotin Conjugated Rabbit Anti-goat IgG (H+L) secondary antibody
BA1006-1	BosterBio	1ml	EUR 157.2

Goat anti-Rabbit IgG-Fc Secondary Antibody (Biotin Conjugate)
GARBBI01	AssayPro	150 ug	EUR 123.6

Goat anti-Rabbit IgG-Fc Secondary Antibody (Biotin Conjugate)
GARBBI02	AssayPro	1 mg	EUR 313.2

Colloidal Gold Conjugate Diluent (10X)
CG-CD10	Alpha Diagnostics	10 ml	EUR 270

Goat anti-Rat IgG Secondary AssayLite Antibody (APC Conjugate)
GARAPC01	AssayPro	150 ug	EUR 513.6

Goat anti-Rat IgG Secondary AssayLite Antibody (RPE Conjugate)
GARRPE01	AssayPro	150 ug	EUR 513.6

Goat anti-Rat IgG Secondary AssayLite Antibody (FITC Conjugate)
GARFITC01	AssayPro	150 ug	EUR 513.6

Goat anti-Rat IgG Secondary AssayLite Antibody (PerCP Conjugate)
GARPERCP01	AssayPro	150 ug	EUR 565.2

Goat anti-Mouse IgG-Fc Secondary AssayLite Antibody (APC Conjugate)
GAMGAPC01	AssayPro	150 ug	EUR 513.6

These data indicate that sustained expression of the human CFTR transgene in the lungs of mice through a delivery vector can be achieved by repeated temporary immunosuppression.