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AFFINITY PURIFIED ANTIBODIES

NathanielAugust 5, 2021

Anti-DYKDDDDK tag agarose is a mouse IgG1 monoclonal antibody covalently coupled in agarose. It is used for the purification or immunoprecipitation of DYKDDDDK tag fusion proteins.

Product Features

1. More stable and superior binding capacity than the competition;
2. Suitable for both N-terminal and C-terminal tagged proteins;
3. Suitable for viscous samples
4. It can be used in column chromatography, batch chromatography, and immunoprecipitation.
5. Locate stocks with strict quality control

Transport:

Sent at room temperature. Store at 2 ~ 8 ℃ or –20 ℃

Storage:

The unopened product is stable for one year from the date of receipt when stored at 2 ~ 8 ℃. After use, the resin should be cleaned and stored in PBS buffer containing ProClin 300 0.03% (v / v) at 2 ~ 8 ℃. Or add 50% glycerol with PBS buffer containing 0.03% (v / v) ProClin 300 stored at -20 ℃. Avoid repeated freezing and thawing. Do not freeze without glycerol.

Formulation:

Anti-DYKDDDDK-tag agarose is supplied as a 50% suspension in PBS (10 mM Na2HPO4, 2.7 mM K2HPO4, 137 mM NaCl, pH 7.4) containing 0.03% ProClin 300 (v / v ).

Reagents required but not supplied:

Binding buffer: PBS (10 mM phosphate, 2.7 mM potassium chloride and 137 mM sodium chloride, pH 7.4)
Elution buffer: 100 mM glycine, 10 mM NaCl, pH 3.0
Neutralization buffer: 1 M Tris-HCl, pH 8-9

Fig.1. Binding capacity comparison between a competitor‘s (A) and Sinobiological’s resin(B)： The competitor‘s (A) and Sinobiological’s resin(B) were incubated with the same amount of DYKDDDDK tagged protein(>2 mg, excessive)and purified according to the instruction respectively. The binding capacity was shown using SDS-PAGE (13%) and UV-Vis.