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AFFINITY PURIFIED ANTIBODIES

Anti-DYKDDDDK tag agarose is a mouse IgG1 monoclonal antibody covalently coupled in agarose. It is used for the purification or immunoprecipitation of DYKDDDDK tag fusion proteins.

Antibody High-affinity mouse IgG1 monoclonal Anti- DYKDDDDK-tag antibody
Agarose 4% cross-linked agarose beads
Particle diameter The particle size range is 45-165 μm, and the average particle size is 90 μm
Capacity ≥0.5mg/mL flag resin (70kDa fusion protein)
Binding Specificity The antibody can bind to DYKDDDDK-tag at the N-terminal, Met-N-terminal, C-terminal, and internal locations of a DYKDDDDK-tag fusion protein
Product status Suspension medium, the bead is white, the solution is colorless
Velocity 0.5-1.0 mL/min is recommended for 1mL column
Pressure ≤ 0.3 MPa

Product Features

1. More stable and superior binding capacity than the competition;
2. Suitable for both N-terminal and C-terminal tagged proteins;
3. Suitable for viscous samples
4. It can be used in column chromatography, batch chromatography, and immunoprecipitation.
5. Locate stocks with strict quality control

Transport:

Sent at room temperature. Store at 2 ~ 8 ℃ or –20 ℃

Storage:

The unopened product is stable for one year from the date of receipt when stored at 2 ~ 8 ℃. After use, the resin should be cleaned and stored in PBS buffer containing ProClin 300 0.03% (v / v) at 2 ~ 8 ℃. Or add 50% glycerol with PBS buffer containing 0.03% (v / v) ProClin 300 stored at -20 ℃. Avoid repeated freezing and thawing. Do not freeze without glycerol.

Formulation:

Anti-DYKDDDDK-tag agarose is supplied as a 50% suspension in PBS (10 mM Na2HPO4, 2.7 mM K2HPO4, 137 mM NaCl, pH 7.4) containing 0.03% ProClin 300 (v / v ).

Reagents required but not supplied:

Binding buffer: PBS (10 mM phosphate, 2.7 mM potassium chloride and 137 mM sodium chloride, pH 7.4)
Elution buffer: 100 mM glycine, 10 mM NaCl, pH 3.0
Neutralization buffer: 1 M Tris-HCl, pH 8-9

Fig.1. Binding capacity comparison between a competitors (A) and Sinobiological’s resin(B): The competitors (A) and Sinobiological’s resin(B) were incubated with the same amount of DYKDDDDK tagged protein(>2 mg, excessive)and purified according to the instruction respectively. The binding capacity was shown using SDS-PAGE (13%) and UV-Vis.

Nathaniel

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