A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.

A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.

can modulate the host immune response to Trichinella spiralis to maintain a suitable environment for long-term survival. Incidentally, the parasite elicits regulatory effects through the release of immunomodulatory molecules, which can suppress the inflammation of the host and can be used for the treatment of inflammatory diseases that are not related to one day. Here we identified and characterized novel T. spiralis cystatin (TsCstN), which inhibits inflammation mediated by LPS-treated macrophages.Proteins contained in the excretion-secretion (ES) muscle-stage products of T. spiralis (ES-L1) fractionated, and each -masing treated with bone marrow-derived macrophages of mice (mBMDMs) before LPS stimulation. The fraction exhibited high immunomodulatory properties by reducing pro-inflammatory cytokines or increase the anti-inflammatory cytokines were identified by mass spectrometry. Incidentally, conserved hypothetical protein (Tsp_04814) were selected for further characterization because it presented the most significant print MS. Annotation of Tsp_04814 using protein structural homology comparisons suggest that it has a high structural similarity to human cystatin E / M (TM score 0.690). T. spiralis new recombinant cystatin (rTsCstN) expressed in Escherichia coli in molecular weight of about 13 kDa. RTsCstN anti-mouse polyclonal antibody (pAb) can detect the native antigen TsCstN raw worms (CWA) and the ES-L1 and will be predominantly localized in stichosome and subcuticular cells. rTsCstN vitro inhibiting cysteine ​​proteases, particularly cathepsin L, the optimal pH 6. Additionally, rTsCstN be internalized into mBMDMs, mostly distributed in the cytoplasm and lysosomes both before and after LPS stimulation. To evaluate the immunomodulatory properties on mBMDMs rTsCstN, rTsCstN incubated with mBMDM before LPS stimulation; This indicates that rTsCstN suppressed pro-inflammatory cytokine production and class II MHC expression.T. spiralis L1 derived TsCstN characterized as new inhibitors of cysteine ​​protease. Protein raises anti-inflammatory properties to suppress pro-inflammatory cytokines and interfere with the process of antigen presentation through depletion of MHC class II expression.

A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.
A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.

Anti-HER2 Affibody-conjugated photosensitizer for photodynamic therapy targeting tumor.

photosensitive molecule antibody can achieve the ideal plus photodynamic tumor-specific therapy (PDT) and indicates strong potential for clinical application. However, some inherent weaknesses, such as circulation during the second half, poor permeation into solid tumors and difficulty in obtaining a uniform coupling products, present a potential problem for clinical applications. In this study, we propose a new design targets the photosensitizer, based on the targeting of very small proteins (molecular affibody) coupled with a photosensitive compound, to overcome this problem.

In synthesis, the photosensitive pyropheophorbide a (Pyro) modified with PEG linker (molecular weight of 727 Da) and then coupled to a site-specific anti-HER2 ZHER2: 2891 affibody protein to provide protein-coupled photosensitizer convenient homogeneous through the process. In vitro and in vivo experiments showed that this molecule has a selectivity that is ideal for binding and photocytotoxicity against cells of HER2-positive (selectivity more than 50-fold between the expression of HER2-high and cell expression of HER2-low) and the accumulation of tumor very specific ; with a relatively low dose, effectively eliminated the high expression of HER2-NCI-N87 tumor in a mouse model.

Goat anti Mouse IgG

40-GM25S 100 ml
EUR 133
Description: Goat anti Mouse IgG secondary antibody

Goat anti Mouse IgG

40-S8301G000-S4 10 ml
EUR 133
Description: Goat anti Mouse IgG secondary antibody

Goat anti Mouse IgG

41-GM25 20 mg
EUR 184
Description: Goat anti Mouse IgG secondary antibody

Goat anti Mouse IgG

41R-1058 2 mg
EUR 149
Description: Goat anti Mouse IgG secondary antibody

Goat anti Mouse IgG

20-B9015G000-S0 10 ml
EUR 133
Description: Goat anti Mouse IgG antibody

Goat anti Mouse IgG

20-S8301G000-V0 10 ml
EUR 133
Description: Goat anti Mouse IgG antibody

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Goat Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-g-48T 48T
EUR 464
Description: A competitive inhibition quantitative ELISA assay kit for detection of Goat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Immunoglobulin G (IgG) ELISA Kit

DLR-IgG-g-96T 96T
EUR 601
Description: A competitive inhibition quantitative ELISA assay kit for detection of Goat Immunoglobulin G (IgG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-g-48Tests 48 Tests
EUR 482

Goat Immunoglobulin G (IgG) ELISA Kit

RDR-IgG-g-96Tests 96 Tests
EUR 667

Goat Anti-Mouse IgG (Fc) IgG, unlabeled

40151-UL 1 mg
EUR 213

Goat Anti-Mouse IgG/HRP

E3GAM001 200ul
EUR 408

Goat Anti Mouse IgG -Biotin

E61I00502 1mg
EUR 499

Goat Anti Mouse IgG-HRP

E61I00503 1mg
EUR 499

Goat Anti Mouse IgG-FITC

E61I00504 1mg
EUR 611

Goat Anti-mouse IgG-ALP

STJ15100031 1 ml
EUR 350
Description: This ALP-conjugate enables detection of mouse IgG in enzyme immunoassays such as indirect ELISA and Western blot.

488 Goat-anti-Mouse IgG

GT26000 1 mg
EUR 375

642 Goat-anti-Mouse IgG

GT26005 1 mg
EUR 375

Goat Anti-Mouse IgG (Fc)

GAMG80Fc-0100 100ml
EUR 404.3

Goat Anti-Mouse IgG (Fc)

GAMG80Fc-0500 500ml
EUR 707.2

Goat Anti-Mouse IgG -HRP

LF-SA8001 0.5 ml
EUR 131
Description: Goat polyclonal to mouse IgG

Goat Anti-Mouse IgG -HRP

LF-SA8001A 1.0 ml
EUR 192
Description: Goat polyclonal to mouse IgG

Goat Anti-Mouse IgG-TRITC

LF-SA8001TR 1.0 mg
EUR 287
Description: Goat polyclonal to mouse IgG

Goat anti Mouse IgG + IgM

41R-1063 1 mg
EUR 137
Description: Goat anti Mouse IgG + IgM secondary antibody

Goat anti Mouse IgG + IgM

41R-1064 1 mg
EUR 194
Description: Goat anti Mouse IgG + IgM secondary antibody

Goat anti Mouse IgG (HRP)

43C-CB1569 1 mg
EUR 198
Description: Goat anti Mouse IgG secondary antibody  (HRP)

Goat anti Mouse IgG (FITC)

43R-1359 2 mg
EUR 186
Description: Goat anti Mouse IgG secondary antibody  (FITC)

Goat anti Mouse IgG (rhodamine)

43R-1360 1 mg
EUR 138
Description: Goat anti Mouse IgG secondary antibody  (rhodamine)

Ibrutinib drug-Human IgG Conjugate

IBT17-IgG 100 ug
EUR 529

Goat Anti-Mouse IgG (Fc) IgG-Biotin Conjugate

40151-BT 1 ml
EUR 225

Goat Anti-Mouse IgG (Fc) IgG-FITC Conjugate

40151-FITC 1 ml
EUR 225

Goat Anti-Mouse IgG (Fc) IgG-HRP Conjugate

40151-HRP 1 ml
EUR 225

Goat Anti-Mouse IgG Fc, 10MG

A008-10MG 10MG
EUR 268

Goat Anti-Mouse IgG Fc, 25MG

A008-25MG 25MG
EUR 486

Goat Anti-Mouse IgG(H+L)

E20-90007 300ul
EUR 343

Goat anti-Mouse IgG(H+L)

E16SPU101-100 100µg
EUR 239

Goat anti-Mouse IgG(H+L)

E16SPU101-1000 1000µg
EUR 434

Goat anti-Mouse IgG(H+L)

E16SPU101-500 500µg
EUR 343

Goat Anti-mouse IgG+IgM-ALP

STJ15100032 1 ml
EUR 350
Description: Goat anti-mouse IgG+IgM-ALP is specific for mouse IgG and IgM H+L-chains and was purified using immunoaffinity chromatography.

Goat Anti-Mouse IgG(h+l)

C020201-100mg 100mg
EUR 6015

Goat Anti-Mouse IgG(h+l)

C020201-1mg 1mg
EUR 185

Goat Anti-Mouse IgG (H+L)

AS071 100 ul
EUR 126

Goat Anti-Mouse IgG Antibody (TRITC)

abx019213-01ml 0.1 ml
EUR 244

Mouse Anti-Goat IgG (Fc) Antibody

abx023336-1mg 1 mg
EUR 370

Goat Anti-Mouse IgG (Fc) Antibody

abx023402-5mg 5 mg
EUR 495

Goat Anti-Mouse IgG (Fc) Antibody

abx023405-2mg 2 mg
EUR 439

Goat Anti-Mouse IgG (Fc) Antibody

abx023407-2mg 2 mg
EUR 411

Goat Anti-Mouse IgG (Fc) Antibody

abx023543-1mg 1 mg
EUR 523

Goat Anti-Mouse IgG Antibody (HRP)

20-abx400001
  • EUR 189.00
  • EUR 112.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg

Goat Anti-Mouse IgG Antibody (Biotin)

20-abx400030
  • EUR 189.00
  • EUR 112.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg

Goat Anti-Mouse IgG Antibody (FITC)

20-abx400031
  • EUR 189.00
  • EUR 112.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg

Goat anti-Mouse IgG Antibody (BGAL)

abx401031-1ml 1 ml
EUR 370

Goat anti-Mouse IgG Antibody (RPE)

abx401034-05mg 0.5 mg
EUR 537

Goat anti-Mouse IgG Fab Antibody

abx401416-1ml 1 ml
EUR 453

Goat anti-Mouse IgG Antibody (RPE)

abx401556-1ml 1 ml
EUR 606

Goat Anti-Mouse IgG-Fab, unlabeled

40154-UL 0.5 mg
EUR 213

Goat Anti-Mouse IgG, aff pure

40319 1 mg
EUR 202

Goat Anti-Mouse IgG, Antisera (Bulk)

40319-GAS-BLK 1-10L, bulk Ask for price

Goat Anti-Mouse IgG-HRP conjugate

40320-200 0.5 ml
EUR 141

Goat Anti-Mouse IgG-FITC conjugate

40330-200 0.5 ml
EUR 141

Goat anti- mouse IgG(H+L)

FNab09848 100µg
EUR 548.75
Description: Antibody raised against Goat mouse IgG(H+L)

Goat Anti-Mouse IgG(H+L)

FNSA-0055 500 uL
EUR 306.6
Description: Goat Anti-Mouse IgG(H+L) secondary antibody

Goat Anti-Mouse IgG(H+L)

FNSA-0060 500 uL
EUR 306.6
Description: Goat Anti-Mouse IgG(H+L) secondary antibody

Goat Anti-Mouse IgG(H+L)

FNSA-0065 500 uL
EUR 306.6
Description: Goat Anti-Mouse IgG(H+L) secondary antibody

Goat Anti-Mouse IgG(H+L)

FNSA-0094 500 uL
EUR 498
Description: Goat Anti-Mouse IgG(H+L) secondary antibody

Goat Anti-Mouse IgG (H&L)

GAMG80-0100 100ml
EUR 360.1

Goat Anti-Mouse IgG (H&L)

GAMG80-0500 500ml
EUR 746.2

Goat Anti-Mouse IgG Fc-AP

LF-SA8001AP 1.0 ml
EUR 332
Description: Goat polyclonal to mouse IgG Fc

Goat Anti-Mouse IgG Fc-BIOT

LF-SA8001B 1.0 mg
EUR 261
Description: Goat polyclonal to mouse IgG Fc

Goat Anti-Mouse IgG Fc-FITC

LF-SA8001F 1.0 mg
EUR 261
Description: Goat polyclonal to mouse IgG Fc

Goat Anti-Mouse IgG Fc-UNLB

LF-SA8001U 1.0 mg
EUR 241
Description: Goat polyclonal to mouse IgG Fc

Goat anti Mouse IgG (H + L)

40-GM25 100 ml
EUR 220
Description: Goat anti Mouse IgG secondary antibody (H + L)

Goat anti Mouse IgG (H + L)

41C-CB1501 2 mg
EUR 216
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (Fab'2)

41C-CJ0207 1 mg
EUR 298
Description: Goat anti Mouse IgG secondary antibody (Fab'2)

Goat anti Mouse IgG (Fab'2)

41C-CJ0213 1 mg
EUR 294
Description: Goat anti Mouse IgG secondary antibody (Fab'2)

Goat anti Mouse IgG (H + L)

41R-1051 2 mg
EUR 149
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1052 2 mg
EUR 137
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1053 2 mg
EUR 149
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1054 2 mg
EUR 194
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1055 2 mg
EUR 309
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1056 1 mg
EUR 153
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1057 1 mg
EUR 186
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (H + L)

41R-1100 2 mg
EUR 219
Description: Goat anti Mouse IgG (H + L) secondary antibody

Goat anti Mouse IgG (Alk Phos)

43R-1357 1 mg
EUR 224
Description: Goat anti Mouse IgG secondary antibody  (Alk Phos)

Goat anti Mouse IgG Fc (biotin)

43R-1358 2 mg
EUR 224
Description: Goat anti Mouse IgG secondary antibody (biotin)

Goat anti Mouse IgG + IgM (FITC)

43R-1388 1 mg
EUR 186
Description: Goat anti Mouse IgG + IgM secondary antibody (FITC)

Goat anti Mouse IgG + IgM (rhodamine)

43R-1389 1 mg
EUR 186
Description: Goat anti Mouse IgG + IgM secondary antibody (rhodamine)

Goat anti Mouse IgG + IgM (HRP)

43R-IG051hrp 1 mg
EUR 198
Description: Goat anti Mouse IgG + IgM secondary antibody (HRP)

Mouse Anti-Goat IgG Antibody [1G12H5]

PM-6279 0.05 mg
EUR 191
Description: Mouse Anti-Goat IgG Antibody [1G12H5]

Goat Anti Human IgG

E61I00201 1mg
EUR 274

Rabbit anti Goat IgG

E61I01002 1mg
EUR 343

goat Anti Rabbit IgG

E61I01301 1mg
EUR 274

Goat Anti-human IgG

STJ15100177 250 µg
EUR 283
Description: Biotinylated goat anti-human IgG enables detection of human IgG in enzyme immunoassays such as indirect ELISA.

Anti-Goat IgG antibody

STJ16101225 1 mg
EUR 191

Anti-Goat IgG antibody

STJ16101226 1.2 mg
EUR 229

Anti-Goat IgG antibody

STJ16101227 1.2 mg
EUR 282

Goat anti- human IgG

FNab09896 100µg Ask for price
Description: Antibody raised against Goat human IgG

Donkey anti Goat IgG

40-DG10 100 ml
EUR 241
Description: Donkey anti Goat IgG secondary antibody

Donkey anti Goat IgG

40-DG10S 100 ml
EUR 133
Description: Donkey anti Goat IgG secondary antibody

Goat anti Rabbit IgG

40-GR30 100 ml
EUR 241
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG

40-GR30S 100 ml
EUR 133
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rat IgG

40-GR38 100 ml
EUR 241
Description: Goat anti Rat IgG secondary antibody

Goat anti Cat IgG

40C-CB0215 2 mg
EUR 271
Description: Goat anti Cat IgG secondary antibody

Goat anti Cat IgG

40C-CB0229 2 mg
EUR 234
Description: Goat anti Cat IgG secondary antibody

Rabbit anti Goat IgG

40C-CB0536 1.5 mg
EUR 269
Description: Rabbit anti Goat IgG secondary antibody

Goat anti Rabbit IgG

40C-CB1157 5 mg
EUR 311
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Monkey IgG

40R-IG002 1 mg
EUR 275
Description: Goat anti Monkey IgG secondary antibody

Goat anti Rabbit IgG

41-GR30 10 mg
EUR 277
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG

41-GR31 5 mg
EUR 162
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Human IgG

41-XG56 1 mg
EUR 101
Description: Goat anti Human IgG secondary antibody

Goat anti Bovine IgG

41C-CB0136 5 mg
EUR 257
Description: Goat anti Bovine IgG secondary antibody

Rabbit anti Goat IgG

41C-CB0529 2 mg
EUR 244
Description: Rabbit anti Goat IgG secondary antibody

Goat anti Human IgG

41C-CB0957 5 mg
EUR 346
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41C-CB0972 2 mg
EUR 264
Description: Goat anti Human IgG secondary antibody

Goat anti Rabbit IgG

41C-CB1143 2 mg
EUR 278
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Rabbit IgG

41C-CB1150 10 mg
EUR 388
Description: Goat anti Rabbit IgG secondary antibody

Goat anti Monkey IgG

41C-CB1605 1 mg
EUR 275
Description: Goat anti Monkey IgG secondary antibody

Goat anti Human IgG

41R-1026 2 mg
EUR 137
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1027 2 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1028 1 mg
EUR 182
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1029 2 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1030 1 mg
EUR 138
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1031 1 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1032 1 mg
EUR 224
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1033 1 mg
EUR 246
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1034 1 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Goat anti Rabbit IgG

41R-1079 2 mg
EUR 149
Description: Goat anti Rabbit IgG secondary antibody

Donkey anti Goat IgG

41R-ID001 1 mg
EUR 229
Description: Donkey anti Goat IgG secondary antibody

Rabbit anti Goat IgG

20-S5801R000-VO 10 ml
EUR 133
Description: Rabbit anti Goat IgG antibody

Goat anti Human IgG

70C-CR6047GAP 1 mg
EUR 149
Description: Affinity purified Goat anti Human IgG antibody

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Goat Mouse IgG

A21010-01ml 0.1ml
EUR 77
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to HRP. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule

Goat Mouse IgG

A21010-05ml 0.5ml
EUR 158
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to HRP. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule

Goat Mouse IgG

A21110-01ml 0.1ml
EUR 93
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to AP. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule

Goat Mouse IgG

A21110-05ml 0.5ml
EUR 224
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to AP. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule

Goat Mouse IgG

A21210-01ml 0.1ml
EUR 77
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to Biotin. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule

Goat Mouse IgG

A21210-05ml 0.5ml
EUR 158
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to Biotin. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule

Goat Mouse IgG

A22110-01ml 0.1ml
EUR 77
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for IF, ICC, FCM. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to FITC. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule

It should be noted that Pyro has only moderate photodynamic activity; However, affibody Pyro-coupled molecules (Pyro-Linker-ZHER2) still shows the function of tumor therapy is excellent.

Nathaniel

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