can modulate the host immune response to Trichinella spiralis to maintain a suitable environment for long-term survival. Incidentally, the parasite elicits regulatory effects through the release of immunomodulatory molecules, which can suppress the inflammation of the host and can be used for the treatment of inflammatory diseases that are not related to one day. Here we identified and characterized novel T. spiralis cystatin (TsCstN), which inhibits inflammation mediated by LPS-treated macrophages.Proteins contained in the excretion-secretion (ES) muscle-stage products of T. spiralis (ES-L1) fractionated, and each -masing treated with bone marrow-derived macrophages of mice (mBMDMs) before LPS stimulation. The fraction exhibited high immunomodulatory properties by reducing pro-inflammatory cytokines or increase the anti-inflammatory cytokines were identified by mass spectrometry. Incidentally, conserved hypothetical protein (Tsp_04814) were selected for further characterization because it presented the most significant print MS. Annotation of Tsp_04814 using protein structural homology comparisons suggest that it has a high structural similarity to human cystatin E / M (TM score 0.690). T. spiralis new recombinant cystatin (rTsCstN) expressed in Escherichia coli in molecular weight of about 13 kDa. RTsCstN anti-mouse polyclonal antibody (pAb) can detect the native antigen TsCstN raw worms (CWA) and the ES-L1 and will be predominantly localized in stichosome and subcuticular cells. rTsCstN vitro inhibiting cysteine proteases, particularly cathepsin L, the optimal pH 6. Additionally, rTsCstN be internalized into mBMDMs, mostly distributed in the cytoplasm and lysosomes both before and after LPS stimulation. To evaluate the immunomodulatory properties on mBMDMs rTsCstN, rTsCstN incubated with mBMDM before LPS stimulation; This indicates that rTsCstN suppressed pro-inflammatory cytokine production and class II MHC expression.T. spiralis L1 derived TsCstN characterized as new inhibitors of cysteine protease. Protein raises anti-inflammatory properties to suppress pro-inflammatory cytokines and interfere with the process of antigen presentation through depletion of MHC class II expression.
A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.
Anti-HER2 Affibody-conjugated photosensitizer for photodynamic therapy targeting tumor.
photosensitive molecule antibody can achieve the ideal plus photodynamic tumor-specific therapy (PDT) and indicates strong potential for clinical application. However, some inherent weaknesses, such as circulation during the second half, poor permeation into solid tumors and difficulty in obtaining a uniform coupling products, present a potential problem for clinical applications. In this study, we propose a new design targets the photosensitizer, based on the targeting of very small proteins (molecular affibody) coupled with a photosensitive compound, to overcome this problem.
In synthesis, the photosensitive pyropheophorbide a (Pyro) modified with PEG linker (molecular weight of 727 Da) and then coupled to a site-specific anti-HER2 ZHER2: 2891 affibody protein to provide protein-coupled photosensitizer convenient homogeneous through the process. In vitro and in vivo experiments showed that this molecule has a selectivity that is ideal for binding and photocytotoxicity against cells of HER2-positive (selectivity more than 50-fold between the expression of HER2-high and cell expression of HER2-low) and the accumulation of tumor very specific ; with a relatively low dose, effectively eliminated the high expression of HER2-NCI-N87 tumor in a mouse model.
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to HRP. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to HRP. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to AP. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to AP. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to Biotin. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for WB, IHC-P, ELISA. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to Biotin. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for IF, ICC, FCM. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to FITC. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for IF, ICC, FCM. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to FITC. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for IF, ICC, FCM. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to Cy3. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule
Description: A polyclonal antibody for detection of Goat IgG from Mouse. This Goat IgG antibody is for IF, ICC, FCM. It is Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE and conjugated to Cy3. The antibody is produced in goat by using as an immunogen Mouse IgG whole molecule
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It should be noted that Pyro has only moderate photodynamic activity; However, affibody Pyro-coupled molecules (Pyro-Linker-ZHER2) still shows the function of tumor therapy is excellent.
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