Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.

Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.

Humulus japonicus (HJ) is a widely used herbal remedy in Asia with anti-oxidative, anti-microbial and anti-inflammatory effects. We investigated the effect of HJ therapeutic potential in rheumatoid arthritis (RA) used a mouse model of collagen-induced arthritis (CIA) and lipopolysaccharide-stimulated murine macrophage cell line (RAW 264.7). CIA rats given 300 mg / kg orally HJ starting 3 days before the second immunization.

Clinical and histopathologic findings assessed in the rat paw CIA. Levels of autoantibodies and inflammatory markers are determined in plasma and cell culture supernatant, respectively. Expression at mRNA and protein levels were analyzed by quantitative reverse transcription-PCR and western blot analysis, respectively. HJ significantly decrease arthritic score dirty and foot swelling in CIA mice. Furthermore, synovial inflammation, cartilage damage and bone erosion that is markedly reduced by HJ.

It also decreased the expression of inflammatory enzymes in both paws of rats and RAW 264.7 cells. Moreover, the expression of genes associated with all pro-inflammatory macrophages and M1 macrophages significantly decreased, whereas the expression of anti-inflammatory M2 macrophage markers was significantly increased in the claws HJ-treated mice CIA. In addition, HJ reduced plasma levels of anti-type II collagen antibody following the decreased expression of T helper type 1 (Th1) and Th2-associated cell surface markers and cytokines in the claws.

HJ also significantly inhibited the expression of IL-6 both in vitro and in vivo, followed by a reduction in STAT3 phosphorylation and expression in rat paw CIA. Finally, the expression of genes associated with decreased osteoclast on HJ-treated rat paw CIA. These findings indicate that the HJ can play a role in suppressing the development of the CIA with the overall regulatory articular inflammation. These studies should provide new insights into the use of the HJ as a natural product effective therapy for RA.

Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.
Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.

Repeat-Dose Toxicity Studies AFPL1-Conjugate Vaccine Using Nicotine in male Sprague Dawley rats.

Tobacco smoking is the cause of 20% of deaths in Canada per year. Nicotine vaccine presents a promising alternative to traditional smoking cessation products, but until now, no vaccine has been able to move through all phases of clinical trials.

We have previously demonstrated that nicotine AFPL1-conjugate vaccine does not cause systemic or immunotoxicity in this mouse model and that heterologous vaccination approach is more profitable than these homologous to stimulate mucosal and systemic antibody anti-nicotine.

The purpose of this study was to confirm the safety profile of the vaccine in the repeated-dose toxicity studies. Heterologous vaccination strategy re-used, and Sprague-Dawley rats given a dose five times greater than in our previous studies.

Anti-HEK293 HCP, affinity purified

HK293R-AF 1 mg
EUR 1913
Description: Anti-HEK293 HCP, affinity purified by Cygnus Technologies is available in Europe via Gentaur.

Anti-HeLa HCP, Affinity Purified

HL59/73-AF 1 mg
EUR 1913
Description: Anti-HeLa HCP, Affinity Purified by Cygnus Technologies is available in Europe via Gentaur.

Goat Anti-Mouse IgG2b, Affinity Purified (Bulk)

40123-GAF-BLK 1g; bulk Ask for price

Goat Anti-Mouse IgG1, Affinity Purified (Bulk)

40126-GAF-BLK 1g; bulk Ask for price

Goat Anti-Mouse IgG2a, Affinity Purified (Bulk)

40127-GAF-BLK 1g; bulk Ask for price

Sheep Anti-Mouse IgG, Affinity Purified (Bulk)

43319-SAF-BLK 1g; bulk Ask for price

Rabbit Anti-Mouse IgG, Affinity Purified (Bulk)

44319-RAF-BLK 1g; bulk Ask for price

Goat Anti-Mouse IgG, Affinity Purified (Bulk)

41319-CAF-BLK 1g; bulk Ask for price

Donkey Anti-Mouse IgG, Affinity Purified (Bulk)

42319-DAF-BLK 1g; bulk Ask for price

Rabbit anti-Mouse Moesin IgG, affinity purified

MSN11-A 100 ul
EUR 482

anti-Dnmt1 (1-248) antibody, affinity-purified

70-201 50ug
EUR 348
Description: The anti-Dnmt1 (1-248) antibody, affinity-purified is available in Europe and for worldwide shipping via Gentaur.

anti-HP1? /CBX5 antibody (rabbit) affinity purified

70-221 50ug
EUR 296
Description: The anti-HP1? /CBX5 antibody (rabbit) affinity purified is available in Europe and for worldwide shipping via Gentaur.

anti-HP1? /CBX1 antibody (rabbit) affinity purified

70-223 50ug
EUR 296
Description: The anti-HP1? /CBX1 antibody (rabbit) affinity purified is available in Europe and for worldwide shipping via Gentaur.

anti-HP1? /CBX3 antibody (rabbit) affinity purified

70-225 50ug Ask for price
Description: The anti-HP1? /CBX3 antibody (rabbit) affinity purified is available in Europe and for worldwide shipping via Gentaur.

Anti-Per.C6Ð’ Affinity Purified HRP, Goat

PC118AF-HRP 1 mg
EUR 4069
Description: Anti-Per.C6Ð’ Affinity Purified HRP, Goat by Cygnus Technologies is available in Europe via Gentaur.

anti-Vero Cell HCP, Affinity Purified

VC807-AFB 1 mg
EUR 3453
Description: anti-Vero Cell HCP, Affinity Purified by Cygnus Technologies is available in Europe via Gentaur.

Anti-Vero Cell HCP affinity purified

VC807.5-AF 0.5 mg
EUR 1605
Description: Anti-Vero Cell HCP affinity purified by Cygnus Technologies is available in Europe via Gentaur.

Mouse Monoclonal Anti-Human IgG, Affinity Purified (Bulk)

10129-MAF-BLK 1g; bulk Ask for price

Rabbit Anti-Human Haptoglobin Polyclonal Antibody(Affinity Purified)

DPAB2047RH 1mg
EUR 580

Modified Affinity Purified Avidin

7-04468 2mg Ask for price

Modified Affinity Purified Avidin

7-04469 10mg Ask for price

Modified Affinity Purified Avidin

7-04470 100mg Ask for price

Goat Anti-Rabbit IgG, Affinity Purified (Bulk)

20318-GAF-BLK 1g; bulk Ask for price

Sheep Anti-Rabbit IgG, Affinity Purified (Bulk)

20319-SAF-BLK 1g; bulk Ask for price

Donkey Anti-Rabbit IgG, Affinity Purified (Bulk)

20320-DAF-BLK 1g; bulk Ask for price

Goat Anti-Human IgA, Affinity Purified (Bulk)

10019-GAF-BLK 1g; bulk Ask for price

Goat Anti-Human IgG, Affinity Purified (Bulk)

10119-GAF-BLK 1g; bulk Ask for price

Rabbit Anti-Human IgG, Affinity Purified (Bulk)

10119-RAF-BLK 1g; bulk Ask for price

Donkey Anti-Sheep IgG, Affinity Purified (Bulk)

30326-CAF-BLK 1g; bulk Ask for price

Monoclonal Anti-Dog IgE, affinity purified, unlabeled

30389 100 ug
EUR 347

Donkey Anti-Sheep IgG, Affinity Purified (Bulk)

30518-DAF-BLK 1g; bulk Ask for price

Goat Anti-Rat IgG, Affinity Purified (Bulk)

50319-GAF-BLK 1g; bulk Ask for price

Donkey Anti-Goat IgG, Affinity Purified (Bulk)

60319-DAF-BLK 1g; bulk Ask for price

Rabbit Anti-Goat IgG, Affinity Purified (Bulk)

60319-RAF-BLK 1g; bulk Ask for price

Sheep Anti-Digoxigenin IgG, unlabeled, affinity purified

DGX11-A 1 mg
EUR 408

Goat Anti-Mouse IgG, Fc Fragment, Affinity Purified (Bulk)

40151-GAF-BLK 1g; bulk Ask for price

Goat Anti-Mouse IgM, µ Chain, Affinity Purified (Bulk)

40221-GAF-BLK 1g; bulk Ask for price

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

dsDNA-cellulose (affinity support) (for removing autoantibodies to dsDNA or DNA-binding proteins)

DNAD15-AS 100 mg
EUR 225

ssDNA-cellulose (affinity support) (for removing autoantibodies to ssDNA or DNA-binding proteins)

DNAS25-AS 100 mg
EUR 225

Modified Affinity Purified Avidin Protein

20-abx263157
  • EUR 1372.00
  • EUR 328.00
  • EUR 230.00
  • 100 mg
  • 10 mg
  • 2 mg

Anti-SF9 Insect Cell HCP 2G affinity purified

SF9RB01-AF 0.5 mg
EUR 1913
Description: Anti-SF9 Insect Cell HCP 2G affinity purified by Cygnus Technologies is available in Europe via Gentaur.

Goat Anti-Human IgM, Myeloma, Affinity Purified (Bulk)

10217-GAF-BLK 1g; bulk Ask for price

Goat Anti-Human IgM, Plasma, Affinity Purified (Bulk)

10218-GAF-BLK 1g; bulk Ask for price

Donkey Anti-Goat IgG (Fc), unlabeled, affinity purified

30230 1 mg
EUR 202

Monoclonal Anti-Dog IgE-Biotin conjugate, affinity purified

30389-B 0.5 ml
EUR 408

Monoclonal Anti-Dog IgE-FITC conjugate, affinity purified

30389-F 0.5 ml
EUR 408

Rabbit Anti-Llama IgG (heavy chain), Affinity Purified

30840-UL 200 ug
EUR 202

Rabbit Anti-Llama IgG (light chain), Affinity Purified

30845-UL 200 ug
EUR 202

Rabbit anti-Pichia pastoris (HCPs) IgG, affinity purified

800-150-PPS 100 ul
EUR 457

Chicken Anti-Protein A IgG, Affinity Purified (Bulk)

PRTA-CAF-BLK 1g; bulk Ask for price

Rabbit anti-Human CD40 IgG, affinity purified, unlabeled

CD4011-A 100 ug
EUR 482

Mouse Monoclonal Anti-Human IgM, clone 1, Affinity Purified (Bulk)

10221-MAF-BLK 1g; bulk Ask for price

Mouse Monoclonal Anti-Human IgM, clone 2, Affinity Purified (Bulk)

10222-MAF-BLK 1g; bulk Ask for price

Glutathione-S-transferase (GST) Affinity Purified Antibody

20-abx137511
  • EUR 829.00
  • EUR 328.00
  • EUR 217.00
  • 100 ug
  • 20 ug
  • 5 ug

Goat Anti-Rabbit IgG, Fc Fragment, Affinity Purified (Bulk)

20119-GAF-BLK 1g; bulk Ask for price

Goat Anti-Human IgG, Fc Fragment, Affinity Purified (Bulk)

10118-GAF-BLK 1g; bulk Ask for price

Goat Anti-Human IgM, µ Chain, Affinity Purified (Bulk)

10219-GAF-BLK 1g; bulk Ask for price

Biotin Modified Affinity Purified Avidin protein

PROTP02701 Regular: 10mg
EUR 317
Description: Avidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and specificity consisting of 128 amino acids and has a molecular mass of about 67K Daltons. ;Avidin-Biotin is a modified form of affinity purified avidin that combines the high affinity specific activity of the native egg white avidin to biotin molecule together with a very low background of the streptavidin produced by the bacteria Streptomyces avidinii.

Goat Anti-Human IgM, Fc (5u specific), Affinity Purified (Bulk)

10216-GAF-BLK 1g; bulk Ask for price

Goat Anti-Llama IgG (H+L chain), Affinity Purified (Bulk)

30817-GAF-BLK 1g; bulk Ask for price

Rabbit Anti-Llama IgG (H+L chain), Affinity Purified (Bulk)

30818-RAF-BLK 1g; bulk Ask for price

Purified bovine histones (H1, H2a, H2b, H3)-Agarose affinity support for removing autoantibdies to histones

HIST15-AS-1 0.5 ml
EUR 347

Purified Human C1q protein for ELISA or Standards

C1Q15-N 100 ug
EUR 250

Purified Human C1q protein for ELISA or Standards

C1Q15-N-1000 1000 ug
EUR 834

Purified, Bovine S100 protein for ELISA or Standards

S1001-R-200 200 ug
EUR 324

Purified, Human S100 protein for ELISA or Standards

S1002-R-100 100 ug
EUR 324

OR Antibody

20-abx217389
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

OR Antibody

44609-100ul 100ul
EUR 252

OR Antibody

44609-50ul 50ul
EUR 187

OR Antibody

DF10250 200ul
EUR 304
Description: OR Antibody detects endogenous levels of total OR.

OR Antibody

ABD10250 100 ug
EUR 438

Dinitrophenyl (DNP)-BSA protein Conjugated to Agarose (affinity matrix)

DNP35-AS-1 1 ml
EUR 347

Mouse anti-Human CD4, Purified mAbConjugated Antibody

C28010 100ul
EUR 397

Mouse anti-Human CD8, Purified mAbConjugated Antibody

C28025 100ul
EUR 397

Mouse anti-Human CD14, Purified mAbConjugated Antibody

C28050 100ul
EUR 397

Mouse anti-Human CD19, Purified mAbConjugated Antibody

C28071 100ul
EUR 397

Mouse anti-Human CD45, Purified mAbConjugated Antibody

C28141 100ul
EUR 397

Monoclonal CD28 Antibody - Purified Ab Conjugated to PE , Clone: CB28

AMM00466G 0.5 ml
EUR 484
Description: A Monoclonal antibody against Human CD28 - Purified Ab Conjugated to PE . The antibodies are raised in Mouse and are from clone CB28. This antibody is applicable in IF, FC

Anti-Cytokeratins Purified

11-108-C025 0.025 mg
EUR 99

Anti-Cytokeratins Purified

11-108-C100 0.1 mg
EUR 158

Anti-MFG Purified

11-113-C025 0.025 mg
EUR 90

Anti-MFG Purified

11-113-C100 0.1 mg
EUR 140

Anti-p53 Purified

11-114-C025 0.025 mg
EUR 104

Anti-p53 Purified

11-114-C100 0.1 mg
EUR 167

Anti-CDK1 Purified

11-115-C025 0.025 mg
EUR 108

Anti-CDK1 Purified

11-115-C100 0.1 mg
EUR 177

Anti-hTSH Purified

11-124-C025 0.025 mg
EUR 90

Anti-hTSH Purified

11-124-C100 0.1 mg
EUR 140

Anti-hTSH Purified

11-125-C025 0.025 mg
EUR 90

Anti-hTSH Purified

11-125-C100 0.1 mg
EUR 140

Anti-hGH Purified

11-126-C025 0.025 mg
EUR 99

Anti-hGH Purified

11-126-C100 0.1 mg
EUR 158

Anti-Lactoferrin Purified

11-128-C025 0.025 mg
EUR 99

Anti-Lactoferrin Purified

11-128-C100 0.1 mg
EUR 158

Anti-Ikaros Purified

11-159-C025 0.025 mg
EUR 99

Anti-Ikaros Purified

11-159-C100 0.1 mg
EUR 158

Anti-PCNA Purified

11-182-C025 0.025 mg
EUR 99

Anti-PCNA Purified

11-182-C100 0.1 mg
EUR 158

Anti-NG2 Purified

11-199-C025 0.025 mg
EUR 99

Anti-NG2 Purified

11-199-C100 0.1 mg
EUR 158

Anti-Insulin Purified

11-246-C025 0.025 mg
EUR 99

Anti-Insulin Purified

11-246-C100 0.1 mg
EUR 158

Anti-MAP2 Purified

11-253-C025 0.025 mg
EUR 113

Anti-MAP2 Purified

11-253-C100 0.1 mg
EUR 186

Anti-Vimentin Purified

11-254-C025 0.025 mg
EUR 113

Anti-Vimentin Purified

11-254-C100 0.1 mg
EUR 186

Anti-GFAP Purified

11-255-C025 0.025 mg
EUR 113

Anti-GFAP Purified

11-255-C100 0.1 mg
EUR 186

Anti-Transferrin Purified

11-258-C025 0.025 mg
EUR 99

Anti-Transferrin Purified

11-258-C100 0.1 mg
EUR 158

Anti-HRP Purified

11-262-C025 0.025 mg
EUR 90

Anti-HRP Purified

11-262-C100 0.1 mg
EUR 140

Anti-Phosphotyrosine Purified

11-263-C025 0.025 mg
EUR 113

Anti-Phosphotyrosine Purified

11-263-C100 0.1 mg
EUR 186

Anti-LCK Purified

11-269-C025 0.025 mg
EUR 113

Anti-LCK Purified

11-269-C100 0.1 mg
EUR 186

Anti-BrdU Purified

11-286-C025 0.025 mg
EUR 108

Anti-BrdU Purified

11-286-C100 0.1 mg
EUR 177

Anti-PSA Purified

11-289-C025 0.025 mg
EUR 90

Anti-PSA Purified

11-289-C100 0.1 mg
EUR 140

Anti-VCP Purified

11-300-C025 0.025 mg
EUR 117

Anti-VCP Purified

11-300-C100 0.1 mg
EUR 195

Anti-GAPDHS Purified

11-301-C025 0.025 mg
EUR 113

Anti-GAPDHS Purified

11-301-C100 0.1 mg
EUR 186

Anti-p21Waf1 Purified

11-338-C025 0.025 mg
EUR 108

Anti-p21Waf1 Purified

11-338-C100 0.1 mg
EUR 177

Anti-TRIM Purified

11-344-C025 0.025 mg
EUR 113

Anti-TRIM Purified

11-344-C100 0.1 mg
EUR 186

Anti-SIT Purified

11-345-C025 0.025 mg
EUR 113

Anti-SIT Purified

11-345-C100 0.1 mg
EUR 186

Anti-PAG1 Purified

11-346-C025 0.025 mg
EUR 122

Anti-PAG1 Purified

11-346-C100 0.1 mg
EUR 204

Anti-PAG1 Purified

11-347-C025 0.025 mg
EUR 122

Anti-PAG1 Purified

11-347-C100 0.1 mg
EUR 204

Anti-NHERF1 Purified

11-348-C025 0.025 mg
EUR 122

Anti-NHERF1 Purified

11-348-C100 0.1 mg
EUR 204

Anti-DAXX Purified

11-351-C025 0.025 mg
EUR 108

Anti-DAXX Purified

11-351-C100 0.1 mg
EUR 177

Anti-LAT Purified

11-360-C025 0.025 mg
EUR 113

Anti-LAT Purified

11-360-C100 0.1 mg
EUR 186

Anti-Vimentin Purified

11-369-C025 0.025 mg
EUR 113

Anti-Vimentin Purified

11-369-C100 0.1 mg
EUR 186

Anti-Tenascin Purified

11-370-C025 0.025 mg
EUR 108

Anti-Tenascin Purified

11-370-C100 0.1 mg
EUR 177

Anti-STAT1 Purified

11-371-C025 0.025 mg
EUR 122

Anti-STAT1 Purified

11-371-C100 0.1 mg
EUR 204

Anti-NTAL Purified

11-373-C025 0.025 mg
EUR 122

Anti-NTAL Purified

11-373-C100 0.1 mg
EUR 204

Anti-NTAL Purified

11-374-C025 0.025 mg
EUR 122

Anti-NTAL Purified

11-374-C100 0.1 mg
EUR 204

Anti-FYN Purified

11-375-C025 0.025 mg
EUR 122

Anti-FYN Purified

11-375-C100 0.1 mg
EUR 204

Anti-SYK Purified

11-376-C025 0.025 mg
EUR 122

Anti-SYK Purified

11-376-C100 0.1 mg
EUR 204

Anti-LYN Purified

11-377-C025 0.025 mg
EUR 122

Anti-LYN Purified

11-377-C100 0.1 mg
EUR 204

Anti-CSK Purified

11-378-R025 0.025 ml
EUR 122

Anti-CSK Purified

11-378-R100 0.1 ml
EUR 204

Anti-LIME1 Purified

11-400-C025 0.025 mg
EUR 122

Anti-LIME1 Purified

11-400-C100 0.1 mg
EUR 204

Anti-LIME1 Purified

11-401-C025 0.025 mg
EUR 122

Anti-LIME1 Purified

11-401-C100 0.1 mg
EUR 204

Anti-SLP76 Purified

11-402-C025 0.025 mg
EUR 122

Anti-SLP76 Purified

11-402-C100 0.1 mg
EUR 204

Anti-STAT1 Purified

11-405-C025 0.025 mg
EUR 122

Anti-STAT1 Purified

11-405-C100 0.1 mg
EUR 204

Anti-SOCS3 Purified

11-406-C025 0.025 mg
EUR 122

Anti-SOCS3 Purified

11-406-C100 0.1 mg
EUR 204

Anti-PAG1 Purified

11-409-C025 0.025 mg
EUR 122

Anti-PAG1 Purified

11-409-C100 0.1 mg
EUR 204

Anti-LIME1 Purified

11-410-C025 0.025 mg
EUR 122

Anti-LIME1 Purified

11-410-C100 0.1 mg
EUR 204

Anti-SLP76 Purified

11-412-C025 0.025 mg
EUR 122

Anti-SLP76 Purified

11-412-C100 0.1 mg
EUR 204

Anti-Clusterin Purified

11-421-C025 0.025 mg
EUR 113

Anti-Clusterin Purified

11-421-C100 0.1 mg
EUR 186

physiological conditions, food and water consumption, body temperature, inflammation of the injection site, relative organ weights, histopathology, and blood chemistry and hematology were evaluated during the vaccination period to determine the safety of the vaccine. AFPL1 nicotine-conjugate vaccine does not induce clinically relevant changes or induces symptoms that would be associated with toxicity, making it a promising candidate for future investigations.

Nathaniel

Related Posts

Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.

A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.

Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans

Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans

The Protein A-mediated binding of Staphylococcus to antibodies in flow cytometric assays and its reduction using FcR blocking reagent

The Protein A-mediated binding of Staphylococcus to antibodies in flow cytometric assays and its reduction using FcR blocking reagent

No Comment

Leave a Reply

Your email address will not be published.

Recent Posts

Categories

September 2022
M T W T F S S
 1234
567891011
12131415161718
19202122232425
2627282930  

Pages