Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.

Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.

Humulus japonicus (HJ) is a widely used herbal remedy in Asia with anti-oxidative, anti-microbial and anti-inflammatory effects. We investigated the effect of HJ therapeutic potential in rheumatoid arthritis (RA) used a mouse model of collagen-induced arthritis (CIA) and lipopolysaccharide-stimulated murine macrophage cell line (RAW 264.7). CIA rats given 300 mg / kg orally HJ starting 3 days before the second immunization.

Clinical and histopathologic findings assessed in the rat paw CIA. Levels of autoantibodies and inflammatory markers are determined in plasma and cell culture supernatant, respectively. Expression at mRNA and protein levels were analyzed by quantitative reverse transcription-PCR and western blot analysis, respectively. HJ significantly decrease arthritic score dirty and foot swelling in CIA mice. Furthermore, synovial inflammation, cartilage damage and bone erosion that is markedly reduced by HJ.

It also decreased the expression of inflammatory enzymes in both paws of rats and RAW 264.7 cells. Moreover, the expression of genes associated with all pro-inflammatory macrophages and M1 macrophages significantly decreased, whereas the expression of anti-inflammatory M2 macrophage markers was significantly increased in the claws HJ-treated mice CIA. In addition, HJ reduced plasma levels of anti-type II collagen antibody following the decreased expression of T helper type 1 (Th1) and Th2-associated cell surface markers and cytokines in the claws.

HJ also significantly inhibited the expression of IL-6 both in vitro and in vivo, followed by a reduction in STAT3 phosphorylation and expression in rat paw CIA. Finally, the expression of genes associated with decreased osteoclast on HJ-treated rat paw CIA. These findings indicate that the HJ can play a role in suppressing the development of the CIA with the overall regulatory articular inflammation. These studies should provide new insights into the use of the HJ as a natural product effective therapy for RA.

Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.
Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.

Repeat-Dose Toxicity Studies AFPL1-Conjugate Vaccine Using Nicotine in male Sprague Dawley rats.

Tobacco smoking is the cause of 20% of deaths in Canada per year. Nicotine vaccine presents a promising alternative to traditional smoking cessation products, but until now, no vaccine has been able to move through all phases of clinical trials.

We have previously demonstrated that nicotine AFPL1-conjugate vaccine does not cause systemic or immunotoxicity in this mouse model and that heterologous vaccination approach is more profitable than these homologous to stimulate mucosal and systemic antibody anti-nicotine.

The purpose of this study was to confirm the safety profile of the vaccine in the repeated-dose toxicity studies. Heterologous vaccination strategy re-used, and Sprague-Dawley rats given a dose five times greater than in our previous studies.

GOAT ANTI MOUSE IGG2, AFFINITY PURIFIED, 100 MG

7455537-100MG 1MG
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SHEEP ANTI MOUSE IGG, AFFINITY PURIFIED, 100 MG

7459507-100MG 1MG
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DONKEY ANTI MOUSE IGG, AFFINITY PURIFIED, 100 MG

7453507-100MG 1MG
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7455547-100MG 1MG
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7455557-100MG 1MG
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7457507-100MG 1MG
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7455507-1G 1G
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GOAT ANTI MOUSE IGG2, AFFINITY PURIFIED, 10 MG

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7455537-1G 1G
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Description: Goat Anti Mouse IgA Polyclonal Affinity Purified

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CHICKEN ANTI MOUSE IGG, AFFINITY PURIFIED, 10 MG

7451507-10MG 1MG
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7451507-1G 1G
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EUR 308
Description: Goat Anti Mouse IgG2a Polyclonal Affinity Purified

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Description: Goat Anti Mouse IgG2b Polyclonal Affinity Purified

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Description: Rabbit Anti Mouse IgA Polyclonal Affinity Purified

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Description: Antibodies

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Description: *Mouse IgG2a Affinity Purified

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GOAT ANTI MOUSE IGM, U CHAIN, AFFINITY PURIFIED, 10 MG

7455567-10MG 1MG
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GOAT ANTI MOUSE IGM, U CHAIN, AFFINITY PURIFIED, 1 G

7455567-1G 1G
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ISHAMSFXGAP100UG each
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Description: *Sheep Anti Mouse Factor X Polyclonal Affinity Purified

*Sheep Anti Mouse Factor X Polyclonal Affinity Purified

ISHAMSFXGAP1MG each
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*Rabbit Anti Mouse Fibronectin Polyclonal Affinity Purified

IRBAMSFBNAP100UG each
EUR 443
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*Rabbit Anti Mouse Vitronectin Polyclonal Affinity Purified

IRBAMSVTNAP100UG each
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*Rabbit Anti Mouse Vitronectin Polyclonal Affinity Purified

IRBAMSVTNAP1MG each
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Description: *Rabbit Anti Mouse Vitronectin Polyclonal Affinity Purified

anti-NS/0 Affinity Purified

N9192-Rb-AF 1 mg
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*Rabbit Anti Mouse Factor XII Polyclonal Affinity Purified

IRBAMSFXIIAP100UG each
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*Rabbit Anti Mouse Factor XII Polyclonal Affinity Purified

IRBAMSFXIIAP1MG each
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Description: *Rabbit Anti Mouse Factor XII Polyclonal Affinity Purified

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10129-MAF-BLK 1g; bulk Ask for price

GOAT ANTI MOUSE IGG, FC FRAGMENT, AFFINITY PURIFIED, 10 MG

7455527-10MG 1MG
EUR 166.65

GOAT ANTI MOUSE IGG, FC FRAGMENT, AFFINITY PURIFIED, 1 G

7455527-1G 1G
EUR 4911.8

Anti-HeLa HCP, Affinity Purified

HL59/73-AF 1 mg
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Anti-MDCK HCP affinity purified

MDCK-Gt-AF 1 mg
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Affinity Purified Anti-MOUSE IgG (H&L) (GOAT)

GWB-3045A4 2 mg Ask for price

Affinity Purified Anti-MOUSE IgG F(c) (GOAT)

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IMSCD1IGGAP10MG each
EUR 178
Description: *Mouse CD1 IgG Affinity Purified

*Mouse CD1 IgG Affinity Purified

IMSCD1IGGAP50MG each
EUR 300
Description: *Mouse CD1 IgG Affinity Purified

MOUSE, IGM, AFFINITY PURIFIED, 100 MG

7404317-100MG 1MG
EUR 1367.22

MOUSE, IGM, AFFINITY PURIFIED, 500 MG

7404317-500MG 1MG
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*Rabbit Anti Mouse Antithrombin III Polyclonal Affinity Purified

IRBAMSATIIIAP100UG each
EUR 368
Description: *Rabbit Anti Mouse Antithrombin III Polyclonal Affinity Purified

*Rabbit Anti Mouse Antithrombin III Polyclonal Affinity Purified

IRBAMSATIIIAP1MG each
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Description: *Rabbit Anti Mouse Antithrombin III Polyclonal Affinity Purified

Affinity Purified Anti-MOUSE (kappa chain) (RABBIT)

GWB-812EA0 1 mg Ask for price

*Rabbit Anti Mouse/Rat Fibrinogen Polyclonal Affinity Purified

IRBAMSFBGAP100UG each
EUR 422
Description: *Rabbit Anti Mouse/Rat Fibrinogen Polyclonal Affinity Purified

*Rabbit Anti Mouse/Rat Fibrinogen Polyclonal Affinity Purified

IRBAMSFBGAP1MG each
EUR 3304
Description: *Rabbit Anti Mouse/Rat Fibrinogen Polyclonal Affinity Purified

*Rabbit Anti Mouse Prorenin/Renin Polyclonal Affinity Purified

IRBAMSPRENAP100UG each
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Description: *Rabbit Anti Mouse Prorenin/Renin Polyclonal Affinity Purified

*Rabbit Anti Mouse Prorenin/Renin Polyclonal Affinity Purified

IRBAMSPRENAP1MG each
EUR 3368
Description: *Rabbit Anti Mouse Prorenin/Renin Polyclonal Affinity Purified

Anti-HEK293 HCP, affinity purified

HK293R-AF 1 mg
EUR 2295.6
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Affinity Purified Anti-MOUSE IgG F(c) (RABBIT)

GWB-5C28AF 2 mg Ask for price

Affinity Purified Anti-MOUSE IgG (H&L) (RABBIT)

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Goat Anti Mouse IgE Polyclonal Affinity Purified HRP Labeled

IGTAMSIGEAPHRP1MG each
EUR 710
Description: Goat Anti Mouse IgE Polyclonal Affinity Purified HRP Labeled

Goat Anti Mouse IgG Polyclonal Affinity Purified HRP Labeled

IGTAMSIGGAPHRP1MG each
EUR 241
Description: Goat Anti Mouse IgG Polyclonal Affinity Purified HRP Labeled

*Rabbit Anti Mouse/Rat Plasminogen Polyclonal Affinity Purified

IRBAMSPLGAP100UG each
EUR 427
Description: *Rabbit Anti Mouse/Rat Plasminogen Polyclonal Affinity Purified

*Rabbit Anti Mouse/Rat Plasminogen Polyclonal Affinity Purified

IRBAMSPLGAP1MG each
EUR 3827
Description: *Rabbit Anti Mouse/Rat Plasminogen Polyclonal Affinity Purified

Mouse Kappa, affinity purified -HRP

GWB-622919 1 mg Ask for price

Goat Anti-Mouse IgG, Affinity Purified (Bulk)

41319-CAF-BLK 1g; bulk Ask for price

Affinity Purified Anti-MOUSE IgM (mu chain) (GOAT)

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Goat Anti Mouse IgG Polyclonal Affinity Purified FITC Labeled

IGTAMSIGGAPFITC1MG each
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Description: Goat Anti Mouse IgG Polyclonal Affinity Purified FITC Labeled

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*Mouse C57BL6 IgG Affinity Purified

IMSC57IGGAP10MG each
EUR 401
Description: *Mouse C57BL6 IgG Affinity Purified

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IMSC57IGGAP50MG each
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Description: *Mouse C57BL6 IgG Affinity Purified

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40126-GAF-BLK 1g; bulk Ask for price

Affinity Purified Anti-MOUSE IgM (mu chain) (RABBIT)

GWB-F5F6AE 2 mg Ask for price

Goat Anti-Mouse IgG2b, Affinity Purified (Bulk)

40123-GAF-BLK 1g; bulk Ask for price

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Donkey Anti-Mouse IgG, Affinity Purified (Bulk)

42319-DAF-BLK 1g; bulk Ask for price

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Affinity Purified Anti-MOUSE IgG F(ab )2 (GOAT)

GWB-969240 2 mg Ask for price

Affinity Purified Anti-BOVINE IgG (H&L) (RABBIT)

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Mouse IgG (H&L), affinity purified

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Goat Anti Mouse Cross Adsorbed IgG Polyclonal Affinity Purified

IGTAMSADIGGAP1MG each
EUR 252
Description: Goat Anti Mouse Cross Adsorbed IgG Polyclonal Affinity Purified

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IGTAMSADIGMAP1MG each
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Description: Goat Anti Mouse Cross Adsorbed IgM Polyclonal Affinity Purified

*Rabbit Anti Mouse/Rat Urokinase (uPA) Polyclonal Affinity Purified

IRBAMSUPAAP100UG each
EUR 528
Description: *Rabbit Anti Mouse/Rat Urokinase (uPA) Polyclonal Affinity Purified

*Rabbit Anti Mouse/Rat Urokinase (uPA) Polyclonal Affinity Purified

IRBAMSUPAAP1MG each
EUR 4263
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*Sheep Anti Mouse/Rat Prorenin/Renin Polyclonal Affinity Purified

ISHAMSRTRENPRENAP100UG each
EUR 389
Description: *Sheep Anti Mouse/Rat Prorenin/Renin Polyclonal Affinity Purified

*Sheep Anti Mouse/Rat Prorenin/Renin Polyclonal Affinity Purified

ISHAMSRTRENPRENAP1MG each
EUR 2830
Description: *Sheep Anti Mouse/Rat Prorenin/Renin Polyclonal Affinity Purified

Rabbit anti-Mouse Moesin IgG, affinity purified

MSN11-A 100 ul
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Affinity Purified Goat Anti-Dog IgE

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IMSBCIGGAP50MG each
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Description: *Mouse Balb C IgG Affinity Purified

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Anti-Per.C6Ð’ Affinity Purified HRP, Goat

PC118AF-HRP 1 mg
EUR 4882.8
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anti-Vero Cell HCP, Affinity Purified

VC807-AFB 1 mg
EUR 4143.6
Description: anti-Vero Cell HCP, Affinity Purified by Cygnus Technologies is available in Europe via Gentaur.

Anti-Vero Cell HCP affinity purified

VC807.5-AF 0.5 mg
EUR 1926
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Affinity Purified Anti-RAT IgM (u) (RABBIT)

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*Sheep Anti Mouse Factor II (Prothrombin) Polyclonal Affinity Purified

ISHAMSFIIAP100UG each
EUR 368
Description: *Sheep Anti Mouse Factor II (Prothrombin) Polyclonal Affinity Purified

*Sheep Anti Mouse Factor II (Prothrombin) Polyclonal Affinity Purified

ISHAMSFIIAP1MG each
EUR 2942
Description: *Sheep Anti Mouse Factor II (Prothrombin) Polyclonal Affinity Purified

Goat Anti Mouse Complement Component 3 Polyclonal Affinity Purified

IGTAMSC3AP100UG each
EUR 310
Description: Goat Anti Mouse Complement Component 3 Polyclonal Affinity Purified

GOAT ANTI RAT IGG, AFFINITY PURIFIED, 100 MG

7455707-100MG 1MG
EUR 910.42

Mouse Monoclonal Anti-Human IgM, clone 1, Affinity Purified (Bulk)

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Rabbit Anti-Human Haptoglobin Polyclonal Antibody(Affinity Purified)

DPAB2047RH 1mg
EUR 696

Affinity Purified Anti-MOUSE IgG IgA IgM (H&L) (GOAT)

GWB-474038 2 mg Ask for price

*Sheep Anti Mouse/Rat tPA Polyclonal Affinity Purified Biotin Labeled

ISHAMSTPAAPBL100UG each
EUR 616
Description: *Sheep Anti Mouse/Rat tPA Polyclonal Affinity Purified Biotin Labeled

*Sheep Anti Mouse/Rat tPA Polyclonal Affinity Purified Biotin Labeled

ISHAMSTPAAPBL1MG each
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Description: *Sheep Anti Mouse/Rat tPA Polyclonal Affinity Purified Biotin Labeled

Affinity Purified Anti-HUMAN IgM (Fc5u) (RABBIT)

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GOAT ANTI HUMAN IGG, AFFINITY PURIFIED, 100 MG

7455407-100MG 1MG
EUR 166.65

GOAT ANTI LLAMA IGG, AFFINITY PURIFIED, 100 MG

7455487-100MG 1MG
EUR 843.07

Affinity Purified Anti-HUMAN (kappa chain) (GOAT)

GWB-4294D3 1 mg Ask for price

Affinity Purified Anti-RAT IgG F(c) (RABBIT)

GWB-412F85 2 mg Ask for price

Affinity Purified Anti-HUMAN IgG (H&L) (GOAT)

GWB-6C40BD 2 mg Ask for price

Affinity Purified Anti-HUMAN IgG F(c) (GOAT)

GWB-DB23A5 5 mg Ask for price

Affinity Purified Anti-MOUSE IgG1 (Gamma 1 chain) (RABBIT)

GWB-49B240 1 mg Ask for price

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DONKEY ANTI GOAT IGG, AFFINITY PURIFIED, 100 MG

7453307-100MG 1MG
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GOAT ANTI RABBIT IGG, AFFINITY PURIFIED, 100 MG

7455607-100MG 1MG
EUR 910.42

RABBIT ANTI GOAT IGG, AFFINITY PURIFIED, 100 MG

7457307-100MG 1MG
EUR 1638.07

Affinity Purified Anti-HUMAN (lambda chain) (GOAT)

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Glutathione-S-transferase (GST) Affinity Purified Antibody

20-abx137511
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Affinity Purified Anti-HUMAN IgG (H&L) (GOAT) (Min X MOUSE Serum Proteins)

GWB-632F65 2 mg Ask for price

Affinity Purified Anti-HUMAN IgG F(c) (GOAT) (Min X MOUSE Serum Proteins)

GWB-20A705 1 mg Ask for price

Affinity Purified Anti-RABBIT IgG (H&L) (GOAT)

GWB-128BDD 2 mg Ask for price

Affinity Purified Anti-RABBIT IgG F(c) (GOAT)

GWB-91AA91 2 mg Ask for price

Affinity Purified Anti-GOAT IgG F(c) (RABBIT)

GWB-9475E1 2 mg Ask for price

Affinity Purified Anti-GOAT IgG (H&L) (RABBIT)

GWB-D8142C 2 mg Ask for price

*Sheep Anti Mouse Neuroserpin Polyclonal Affinity Purified Biotin Labeled

ISHAMSNSPAPBL100UG each
EUR 616
Description: *Sheep Anti Mouse Neuroserpin Polyclonal Affinity Purified Biotin Labeled

*Sheep Anti Mouse Neuroserpin Polyclonal Affinity Purified Biotin Labeled

ISHAMSNSPAPBL1MG each
EUR 5180
Description: *Sheep Anti Mouse Neuroserpin Polyclonal Affinity Purified Biotin Labeled

Mouse Anti 6X-Histidine Tag Monoclonal Affinity Purified Lyophilized

IMSA6XHIS100UG each
EUR 339
Description: Mouse Anti 6X-Histidine Tag Monoclonal Affinity Purified Lyophilized

Mouse Anti 6X-Histidine Tag Monoclonal Affinity Purified Lyophilized

IMSA6XHIS1MG each
EUR 1476
Description: Mouse Anti 6X-Histidine Tag Monoclonal Affinity Purified Lyophilized

Affinity Purified Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT)

GWB-185E67 1 mg Ask for price

Affinity Purified Anti-MOUSE IgG2b (Gamma 2b chain) (RABBIT)

GWB-C17D96 1 mg Ask for price

physiological conditions, food and water consumption, body temperature, inflammation of the injection site, relative organ weights, histopathology, and blood chemistry and hematology were evaluated during the vaccination period to determine the safety of the vaccine. AFPL1 nicotine-conjugate vaccine does not induce clinically relevant changes or induces symptoms that would be associated with toxicity, making it a promising candidate for future investigations.

Nathaniel

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