Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.

Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.

Humulus japonicus (HJ) is a widely used herbal remedy in Asia with anti-oxidative, anti-microbial and anti-inflammatory effects. We investigated the effect of HJ therapeutic potential in rheumatoid arthritis (RA) used a mouse model of collagen-induced arthritis (CIA) and lipopolysaccharide-stimulated murine macrophage cell line (RAW 264.7). CIA rats given 300 mg / kg orally HJ starting 3 days before the second immunization.

Clinical and histopathologic findings assessed in the rat paw CIA. Levels of autoantibodies and inflammatory markers are determined in plasma and cell culture supernatant, respectively. Expression at mRNA and protein levels were analyzed by quantitative reverse transcription-PCR and western blot analysis, respectively. HJ significantly decrease arthritic score dirty and foot swelling in CIA mice. Furthermore, synovial inflammation, cartilage damage and bone erosion that is markedly reduced by HJ.

It also decreased the expression of inflammatory enzymes in both paws of rats and RAW 264.7 cells. Moreover, the expression of genes associated with all pro-inflammatory macrophages and M1 macrophages significantly decreased, whereas the expression of anti-inflammatory M2 macrophage markers was significantly increased in the claws HJ-treated mice CIA. In addition, HJ reduced plasma levels of anti-type II collagen antibody following the decreased expression of T helper type 1 (Th1) and Th2-associated cell surface markers and cytokines in the claws.

HJ also significantly inhibited the expression of IL-6 both in vitro and in vivo, followed by a reduction in STAT3 phosphorylation and expression in rat paw CIA. Finally, the expression of genes associated with decreased osteoclast on HJ-treated rat paw CIA. These findings indicate that the HJ can play a role in suppressing the development of the CIA with the overall regulatory articular inflammation. These studies should provide new insights into the use of the HJ as a natural product effective therapy for RA.

Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.
Humulus japonicus extract ameliorates collagen‑induced arthritis in mice through regulation of overall articular inflammation.

Repeat-Dose Toxicity Studies AFPL1-Conjugate Vaccine Using Nicotine in male Sprague Dawley rats.

Tobacco smoking is the cause of 20% of deaths in Canada per year. Nicotine vaccine presents a promising alternative to traditional smoking cessation products, but until now, no vaccine has been able to move through all phases of clinical trials.

We have previously demonstrated that nicotine AFPL1-conjugate vaccine does not cause systemic or immunotoxicity in this mouse model and that heterologous vaccination approach is more profitable than these homologous to stimulate mucosal and systemic antibody anti-nicotine.

The purpose of this study was to confirm the safety profile of the vaccine in the repeated-dose toxicity studies. Heterologous vaccination strategy re-used, and Sprague-Dawley rats given a dose five times greater than in our previous studies.

Anti-HEK293 HCP, affinity purified

HK293R-AF 1 mg
EUR 2295.6
Description: Anti-HEK293 HCP, affinity purified by Cygnus Technologies is available in Europe via Gentaur.

Anti-HeLa HCP, Affinity Purified

HL59/73-AF 1 mg
EUR 2295.6
Description: Anti-HeLa HCP, Affinity Purified by Cygnus Technologies is available in Europe via Gentaur.

Sheep Anti-Mouse IgG, Affinity Purified (Bulk)

43319-SAF-BLK 1g; bulk Ask for price

Rabbit Anti-Mouse IgG, Affinity Purified (Bulk)

44319-RAF-BLK 1g; bulk Ask for price

Donkey Anti-Mouse IgG, Affinity Purified (Bulk)

42319-DAF-BLK 1g; bulk Ask for price

Goat Anti-Mouse IgG, Affinity Purified (Bulk)

41319-CAF-BLK 1g; bulk Ask for price

Goat Anti-Mouse IgG2b, Affinity Purified (Bulk)

40123-GAF-BLK 1g; bulk Ask for price

Goat Anti-Mouse IgG1, Affinity Purified (Bulk)

40126-GAF-BLK 1g; bulk Ask for price

Goat Anti-Mouse IgG2a, Affinity Purified (Bulk)

40127-GAF-BLK 1g; bulk Ask for price

Rabbit anti-Mouse Moesin IgG, affinity purified

MSN11-A 100 ul
EUR 578.4

anti-Dnmt1 (1-248) antibody, affinity-purified

70-201 50ug
EUR 417.6
Description: The anti-Dnmt1 (1-248) antibody, affinity-purified is available in Europe and for worldwide shipping via Gentaur.

anti-HP1? /CBX5 antibody (rabbit) affinity purified

70-221 50ug
EUR 355.2
Description: The anti-HP1? /CBX5 antibody (rabbit) affinity purified is available in Europe and for worldwide shipping via Gentaur.

anti-HP1? /CBX1 antibody (rabbit) affinity purified

70-223 50ug
EUR 355.2
Description: The anti-HP1? /CBX1 antibody (rabbit) affinity purified is available in Europe and for worldwide shipping via Gentaur.

anti-HP1? /CBX3 antibody (rabbit) affinity purified

70-225 50ug Ask for price
Description: The anti-HP1? /CBX3 antibody (rabbit) affinity purified is available in Europe and for worldwide shipping via Gentaur.

Anti-Per.C6Ð’ Affinity Purified HRP, Goat

PC118AF-HRP 1 mg
EUR 4882.8
Description: Anti-Per.C6Ð’ Affinity Purified HRP, Goat by Cygnus Technologies is available in Europe via Gentaur.

anti-Vero Cell HCP, Affinity Purified

VC807-AFB 1 mg
EUR 4143.6
Description: anti-Vero Cell HCP, Affinity Purified by Cygnus Technologies is available in Europe via Gentaur.

Anti-Vero Cell HCP affinity purified

VC807.5-AF 0.5 mg
EUR 1926
Description: Anti-Vero Cell HCP affinity purified by Cygnus Technologies is available in Europe via Gentaur.

Mouse Monoclonal Anti-Human IgG, Affinity Purified (Bulk)

10129-MAF-BLK 1g; bulk Ask for price

Modified Affinity Purified Avidin

7-04469 10mg Ask for price

Modified Affinity Purified Avidin

7-04470 100mg Ask for price

Modified Affinity Purified Avidin

7-04468 2mg Ask for price

Rabbit Anti-Human Haptoglobin Polyclonal Antibody(Affinity Purified)

DPAB2047RH 1mg
EUR 696

Donkey Anti-Goat IgG, Affinity Purified (Bulk)

60319-DAF-BLK 1g; bulk Ask for price

Rabbit Anti-Goat IgG, Affinity Purified (Bulk)

60319-RAF-BLK 1g; bulk Ask for price

Goat Anti-Rat IgG, Affinity Purified (Bulk)

50319-GAF-BLK 1g; bulk Ask for price

Goat Anti-Human IgA, Affinity Purified (Bulk)

10019-GAF-BLK 1g; bulk Ask for price

Goat Anti-Human IgG, Affinity Purified (Bulk)

10119-GAF-BLK 1g; bulk Ask for price

Rabbit Anti-Human IgG, Affinity Purified (Bulk)

10119-RAF-BLK 1g; bulk Ask for price

Donkey Anti-Sheep IgG, Affinity Purified (Bulk)

30326-CAF-BLK 1g; bulk Ask for price

Monoclonal Anti-Dog IgE, affinity purified, unlabeled

30389 100 ug
EUR 416.4

Donkey Anti-Sheep IgG, Affinity Purified (Bulk)

30518-DAF-BLK 1g; bulk Ask for price

Goat Anti-Rabbit IgG, Affinity Purified (Bulk)

20318-GAF-BLK 1g; bulk Ask for price

Sheep Anti-Rabbit IgG, Affinity Purified (Bulk)

20319-SAF-BLK 1g; bulk Ask for price

Donkey Anti-Rabbit IgG, Affinity Purified (Bulk)

20320-DAF-BLK 1g; bulk Ask for price

Sheep Anti-Digoxigenin IgG, unlabeled, affinity purified

DGX11-A 1 mg
EUR 489.6

Goat Anti-Mouse IgG, Fc Fragment, Affinity Purified (Bulk)

40151-GAF-BLK 1g; bulk Ask for price

Goat Anti-Mouse IgM, µ Chain, Affinity Purified (Bulk)

40221-GAF-BLK 1g; bulk Ask for price

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

Modified Affinity Purified Avidin Protein

20-abx263157
  • EUR 1646.40
  • EUR 393.60
  • EUR 276.00
  • 100 mg
  • 10 mg
  • 2 mg

Donkey Anti-Goat IgG (Fc), unlabeled, affinity purified

30230 1 mg
EUR 242.4

Monoclonal Anti-Dog IgE-Biotin conjugate, affinity purified

30389-B 0.5 ml
EUR 489.6

Monoclonal Anti-Dog IgE-FITC conjugate, affinity purified

30389-F 0.5 ml
EUR 489.6

Rabbit Anti-Llama IgG (heavy chain), Affinity Purified

30840-UL 200 ug
EUR 242.4

Rabbit Anti-Llama IgG (light chain), Affinity Purified

30845-UL 200 ug
EUR 242.4

Goat Anti-Human IgM, Myeloma, Affinity Purified (Bulk)

10217-GAF-BLK 1g; bulk Ask for price

Goat Anti-Human IgM, Plasma, Affinity Purified (Bulk)

10218-GAF-BLK 1g; bulk Ask for price

Rabbit anti-Pichia pastoris (HCPs) IgG, affinity purified

800-150-PPS 100 ul
EUR 548.4

Rabbit anti-Human CD40 IgG, affinity purified, unlabeled

CD4011-A 100 ug
EUR 578.4

Chicken Anti-Protein A IgG, Affinity Purified (Bulk)

PRTA-CAF-BLK 1g; bulk Ask for price

Anti-SF9 Insect Cell HCP 2G affinity purified

SF9RB01-AF 0.5 mg
EUR 2295.6
Description: Anti-SF9 Insect Cell HCP 2G affinity purified by Cygnus Technologies is available in Europe via Gentaur.

Mouse Monoclonal Anti-Human IgM, clone 1, Affinity Purified (Bulk)

10221-MAF-BLK 1g; bulk Ask for price

Mouse Monoclonal Anti-Human IgM, clone 2, Affinity Purified (Bulk)

10222-MAF-BLK 1g; bulk Ask for price

Glutathione-S-transferase (GST) Affinity Purified Antibody

20-abx137511
  • EUR 994.80
  • EUR 393.60
  • EUR 260.40
  • 100 ug
  • 20 ug
  • 5 ug

dsDNA-cellulose (affinity support) (for removing autoantibodies to dsDNA or DNA-binding proteins)

DNAD15-AS 100 mg
EUR 270

ssDNA-cellulose (affinity support) (for removing autoantibodies to ssDNA or DNA-binding proteins)

DNAS25-AS 100 mg
EUR 270

Biotin Modified Affinity Purified Avidin protein

PROTP02701 Regular: 10mg
EUR 380.4
Description: Avidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and specificity consisting of 128 amino acids and has a molecular mass of about 67K Daltons. ;Avidin-Biotin is a modified form of affinity purified avidin that combines the high affinity specific activity of the native egg white avidin to biotin molecule together with a very low background of the streptavidin produced by the bacteria Streptomyces avidinii.

Goat Anti-Human IgG, Fc Fragment, Affinity Purified (Bulk)

10118-GAF-BLK 1g; bulk Ask for price

Goat Anti-Rabbit IgG, Fc Fragment, Affinity Purified (Bulk)

20119-GAF-BLK 1g; bulk Ask for price

Goat Anti-Human IgM, µ Chain, Affinity Purified (Bulk)

10219-GAF-BLK 1g; bulk Ask for price

Goat Anti-Llama IgG (H+L chain), Affinity Purified (Bulk)

30817-GAF-BLK 1g; bulk Ask for price

Rabbit Anti-Llama IgG (H+L chain), Affinity Purified (Bulk)

30818-RAF-BLK 1g; bulk Ask for price

Goat Anti-Human IgM, Fc (5u specific), Affinity Purified (Bulk)

10216-GAF-BLK 1g; bulk Ask for price

Purified Human C1q protein for ELISA or Standards

C1Q15-N 100 ug
EUR 300

Purified Human C1q protein for ELISA or Standards

C1Q15-N-1000 1000 ug
EUR 1000.8

Purified, Bovine S100 protein for ELISA or Standards

S1001-R-200 200 ug
EUR 388.8

Purified, Human S100 protein for ELISA or Standards

S1002-R-100 100 ug
EUR 388.8

Purified bovine histones (H1, H2a, H2b, H3)-Agarose affinity support for removing autoantibdies to histones

HIST15-AS-1 0.5 ml
EUR 416.4

Purified Anti-mouse CD19 Antibody *1D3*

10194000 100 ug
EUR 164

Purified Anti-mouse CD197 Antibody *4B12*

11970000 100 ug
EUR 365

OR Antibody

44609-100ul 100ul
EUR 302.4

OR Antibody

44609-50ul 50ul
EUR 224.4

OR Antibody

ABD10250 100 ug
EUR 525.6

OR Antibody

20-abx217389
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

OR Antibody

DF10250 200ul
EUR 420

Mouse anti-Human CD4, Purified mAbConjugated Antibody

C28010 100ul
EUR 476.4

Mouse anti-Human CD8, Purified mAbConjugated Antibody

C28025 100ul
EUR 476.4

Mouse anti-Human CD14, Purified mAbConjugated Antibody

C28050 100ul
EUR 476.4

Mouse anti-Human CD19, Purified mAbConjugated Antibody

C28071 100ul
EUR 476.4

Mouse anti-Human CD45, Purified mAbConjugated Antibody

C28141 100ul
EUR 476.4

Purified Anti-mouse CD105 Antibody *MJ7/18*

11050000 100 ug
EUR 365

Purified Anti-mouse CD106 Antibody *429 (MVCAM.A)*

11060000 100 ug
EUR 365

Purified Anti-mouse CD26 Antibody *H194-112*

10260000 100 ug
EUR 365

Purified Anti-human/mouse CD44 Antibody *IM7*

10441000 100 ug
EUR 164

Purified Anti-mouse CD86 Antibody *GL-1*

10860000 100 ug
EUR 365

Anti-Flag Affinity Gel

B23101 1 ml Kit
EUR 579.6
Description: Anti-Flag Affinity Gel is a Protein A purIFied murine IgG2b Monoclonal antibody covalently attached to sepharose 4B wHICh could used on Flag fusion protein purIFication, IP and CO-IP.

Anti-Flag Affinity Gel

B23102 5 ml Kit
EUR 1742.4
Description: Anti-Flag Affinity Gel is a Protein A purIFied murine IgG2b Monoclonal antibody covalently attached to sepharose 4B wHICh could used on Flag fusion protein purIFication, IP and CO-IP.

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 336

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

Anti-SOS Purified

11-522-C025 0.025 mg
EUR 129.6

Anti-SOS Purified

11-522-C100 0.1 mg
EUR 212.4

Anti-PKAc Purified

11-523-C025 0.025 mg
EUR 129.6

Anti-PKAc Purified

11-523-C100 0.1 mg
EUR 212.4

Anti-SHIP1 Purified

11-524-C025 0.025 mg
EUR 129.6

Anti-SHIP1 Purified

11-524-C100 0.1 mg
EUR 212.4

Anti-SHIP1 Purified

11-525-C025 0.025 mg
EUR 129.6

Anti-SHIP1 Purified

11-525-C100 0.1 mg
EUR 212.4

Anti-LST1 Purified

11-528-C025 0.025 mg
EUR 146.4

Anti-LST1 Purified

11-528-C100 0.1 mg
EUR 244.8

Anti-PSMA Purified

11-532-C025 0.025 mg
EUR 151.2

Anti-PSMA Purified

11-532-C100 0.1 mg
EUR 255.6

Anti-CRP Purified

11-537-C100 0.1 mg
EUR 244.8

Anti-PSMA Purified

11-539-C025 0.025 mg
EUR 151.2

Anti-PSMA Purified

11-539-C100 0.1 mg
EUR 255.6

Anti-AHNAK1 Purified

11-540-C025 0.025 mg
EUR 146.4

Anti-AHNAK1 Purified

11-540-C100 0.1 mg
EUR 244.8

Anti-BRG1 Purified

11-541-C025 0.025 mg
EUR 146.4

Anti-BRG1 Purified

11-541-C100 0.1 mg
EUR 244.8

Anti-CPNE7 Purified

11-542-C025 0.025 mg
EUR 151.2

Anti-CPNE7 Purified

11-542-C100 0.1 mg
EUR 255.6

Anti-GRB2 Purified

11-554-C025 0.025 mg
EUR 146.4

Anti-GRB2 Purified

11-554-C100 0.1 mg
EUR 244.8

Anti-AGR3 Purified

11-560-C025 0.025 mg
EUR 178.8

Anti-AGR3 Purified

11-560-C100 0.1 mg
EUR 309.6

Anti-AGR3 Purified

11-561-C025 0.025 mg
EUR 178.8

Anti-AGR3 Purified

11-561-C100 0.1 mg
EUR 309.6

Anti-UBN1 Purified

11-565-C025 0.025 mg
EUR 146.4

Anti-UBN1 Purified

11-565-C100 0.1 mg
EUR 244.8

Anti-GFAP Purified

11-583-C025 0.025 mg
EUR 135.6

Anti-GFAP Purified

11-583-C100 0.1 mg
EUR 223.2

Anti-FoxP3 Purified

11-601-C025 0.025 mg
EUR 163.2

Anti-FoxP3 Purified

11-601-C100 0.1 mg
EUR 277.2

Anti-PRR7 Purified

11-617-C025 0.025 mg
EUR 168

Anti-PRR7 Purified

11-617-C100 0.1 mg
EUR 288

Anti-ABRA1 Purified

11-619-C025 0.025 mg
EUR 146.4

Anti-ABRA1 Purified

11-619-C100 0.1 mg
EUR 244.8

Anti-GRAP2 Purified

11-639-C025 0.025 mg
EUR 146.4

Anti-GRAP2 Purified

11-639-C100 0.1 mg
EUR 244.8

Anti-Notch1 Purified

11-651-C025 0.025 mg
EUR 118.8

Anti-Notch1 Purified

11-651-C100 0.1 mg
EUR 189.6

Anti-PDK1 Purified

11-659-C100 0.1 mg
EUR 244.8

Anti-ZAP70 Purified

11-665-C025 0.025 mg
EUR 129.6

Anti-ZAP70 Purified

11-665-C100 0.1 mg
EUR 212.4

Anti-EGFR Purified

11-680-C025 0.025 mg
EUR 140.4

Anti-EGFR Purified

11-680-C100 0.1 mg
EUR 234

Anti-BrdU Purified

11-682-C025 0.025 mg
EUR 129.6

Anti-BrdU Purified

11-682-C100 0.1 mg
EUR 212.4

Anti-Nitrotyrosine Purified

11-693-C025 0.025 mg
EUR 189.6

Anti-Nitrotyrosine Purified

11-693-C100 0.1 mg
EUR 332.4

Anti-Drebrin Purified

11-694-C025 0.025 mg
EUR 146.4

Anti-Drebrin Purified

11-694-C100 0.1 mg
EUR 244.8

Anti-FOLR2 Purified

11-696-C025 0.025 mg
EUR 118.8

Anti-FOLR2 Purified

11-696-C100 0.1 mg
EUR 189.6

Anti-PCLO Purified

11-698-C025 0.025 mg
EUR 146.4

Anti-PCLO Purified

11-698-C100 0.1 mg
EUR 244.8

Anti-OPAL1 Purified

11-699-C025 0.025 mg
EUR 146.4

Anti-OPAL1 Purified

11-699-C100 0.1 mg
EUR 244.8

Anti-CLIC5 Purified

11-701-C025 0.025 mg
EUR 146.4

Anti-CLIC5 Purified

11-701-C100 0.1 mg
EUR 244.8

Anti-LARGE1 Purified

11-702-C025 0.025 mg
EUR 146.4

Anti-LARGE1 Purified

11-702-C100 0.1 mg
EUR 244.8

Anti-SCIMP Purified

11-704-C025 0.025 mg
EUR 146.4

Anti-SCIMP Purified

11-704-C100 0.1 mg
EUR 244.8

Anti-TFG Purified

11-709-C025 0.025 mg
EUR 146.4

Anti-TFG Purified

11-709-C100 0.1 mg
EUR 244.8

Anti-PDE8A Purified

11-710-C025 0.025 mg
EUR 146.4

Anti-PDE8A Purified

11-710-C100 0.1 mg
EUR 244.8

Anti-NCK1 Purified

11-711-C025 0.025 mg
EUR 146.4

Anti-NCK1 Purified

11-711-C100 0.1 mg
EUR 244.8

Anti-DLL4 Purified

11-717-C025 0.025 mg
EUR 103.2

Anti-DLL4 Purified

11-717-C100 0.1 mg
EUR 157.2

Anti-RLTPR Purified

11-718-C025 0.025 mg
EUR 146.4

Anti-RLTPR Purified

11-718-C100 0.1 mg
EUR 244.8

Anti-NR2F6 Purified

11-719-C025 0.025 mg
EUR 146.4

Anti-NR2F6 Purified

11-719-C100 0.1 mg
EUR 244.8

Anti-ARHGEF4 Purified

11-723-C025 0.025 mg
EUR 129.6

Anti-ARHGEF4 Purified

11-723-C100 0.1 mg
EUR 212.4

Anti-DDIT4L Purified

11-725-C025 0.025 mg
EUR 146.4

Anti-DDIT4L Purified

11-725-C100 0.1 mg
EUR 244.8

Anti-AGPS Purified

11-739-C025 0.025 mg
EUR 146.4

Anti-AGPS Purified

11-739-C100 0.1 mg
EUR 244.8

Anti-LRRC32 Purified

11-744-C025 0.025 mg
EUR 118.8

Anti-LRRC32 Purified

11-744-C100 0.1 mg
EUR 189.6

Anti-DR3 Purified

11-757-C025 0.025 mg
EUR 118.8

Anti-DR3 Purified

11-757-C100 0.1 mg
EUR 189.6

Anti-Perforin Purified

11-765-C025 0.025 mg
EUR 129.6

Anti-Perforin Purified

11-765-C100 0.1 mg
EUR 212.4

physiological conditions, food and water consumption, body temperature, inflammation of the injection site, relative organ weights, histopathology, and blood chemistry and hematology were evaluated during the vaccination period to determine the safety of the vaccine. AFPL1 nicotine-conjugate vaccine does not induce clinically relevant changes or induces symptoms that would be associated with toxicity, making it a promising candidate for future investigations.

Nathaniel

Related Posts

Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

Development of flow cytometry assays for measuring cell-membrane enzyme activity on individual cells.

A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.

A novel cystatin derived from Trichinella spiralis suppresses macrophage-mediated inflammatory responses.

Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans

Complement Receptor 2 Based Immunoassay Measuring Activation of the Complement System at C3-Level in Plasma Samples From Mice and Humans

The Protein A-mediated binding of Staphylococcus to antibodies in flow cytometric assays and its reduction using FcR blocking reagent

The Protein A-mediated binding of Staphylococcus to antibodies in flow cytometric assays and its reduction using FcR blocking reagent

No Comment

Leave a Reply

Your email address will not be published.

Recent Posts

Categories

May 2023
M T W T F S S
1234567
891011121314
15161718192021
22232425262728
293031  

Pages